Detailed information NTG1074656

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: ACCH06_RS05820
• Genome accession: NZ_CP174157
• Coordinates: 1195490..1199194 (+)
• Length: 1234 a.a.
• NCBI ID: WP_406589855.1
• Uniprot ID: -
• Organism: Bacillus atrophaeus strain SHZ-24
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1190490..1204194

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACCH06_RS05805	-	1190939..1191264 (-)	326	Protein_1087	four-helix bundle copper-binding protein	-
ACCH06_RS05810	-	1191361..1191798 (-)	438	WP_258728959.1	ferritin family protein	-
ACCH06_RS05815	addB	1192003..1195503 (+)	3501	WP_406589854.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
ACCH06_RS05820	addA	1195490..1199194 (+)	3705	WP_406589855.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
ACCH06_RS05825	sbcD	1199265..1200443 (+)	1179	WP_406589856.1	exonuclease subunit SbcD	-
ACCH06_RS05830	sbcC	1200440..1203832 (+)	3393	WP_406589857.1	exonuclease subunit SbcC	-
ACCH06_RS05835	-	1203849..1204151 (+)	303	WP_003327677.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141476.05 Da
• Isoelectric Point: 5.4163

>NTDB_id=1074656 ACCH06_RS05820 WP_406589855.1 1195490..1199194(+) (addA) [Bacillus atrophaeus strain SHZ-24]
MQIPKPADSTWTDDQWNAIVSSGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLRKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPKTWLEGFVHLYDASEKSTIEELPFYPYMKEDIAMVLSAVKEKLQRA
LELTKVPGGPAPRADNFLDDLAQTEDLIAHSADFTELYKRVPAVSFKRAKAVKGDEFDQALTEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLSDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAVNESGERKPSEAAAYYQEQ
FQEVLVDEYQDTNLVQEAILQLVTSGPEASGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTETGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKVGEVDYDEQAELKLGASYPPNDQTETELLLIDRAEEKEASEETEELETVQLEAKAIAKQIR
KLVSSPYKVYDGKSKTHSNIRYRDIVILLRSMPWAPQIMEELKAQGIPVYANLTSGYFEAVEVSVAISALKVIDNPYQDI
PLASVLRSPIVGADENELSLIRMHNKKAPFYEAMKDYLAGGDRNDELYQKLNTFFELLQKWRSFSKNHSVSELIWEVYRD
SKYMDYVGGMPGGKQRQANLRVLYDRSRQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQASASQPDWLLPDFERYQAKTYLDFIGPALIRHRDMGTDALQGLASQADISEHPARFNIQMVSAQD
LLDDDLEERQEEKSGRLDAIRRGEPVSGSFEFEEQARQRLSWRYPYHDVTKIRTKQTVSEIKRKKEYEDEYSGRSLIRPA
DGNMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHAPSKEEAERIVKRLYEKELLTEDQQAVIDLEEIVQFFHTEIGE
KLIGAKWINREVPFSMALSAQDAFLDTESKDEPILVQGIIDCLYETDEGLFLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIELYTKAVEQITKTNVKGRALYFFDGGHVLNV

Nucleotide

• Length: 3705 bp

>NTDB_id=1074656 ACCH06_RS05820 WP_406589855.1 1195490..1199194(+) (addA) [Bacillus atrophaeus strain SHZ-24]
ATGCAAATTCCTAAACCGGCAGACAGCACCTGGACAGATGACCAGTGGAACGCCATTGTCTCATCCGGACAGGATATTCT
CGTTGCAGCGGCCGCTGGATCGGGAAAAACCGCTGTGCTTGTTGAGAGAATGATCCGAAAAATTACCGCGGAGGAAAATC
CGATAGATGTGGACCGTCTTTTGGTCGTGACATTTACGAACGCCTCTGCGGCTGAAATGAAGCACCGGATCGCGGAGGCT
TTGGAGAAAGAGCTCGTGAAACGTCCCGGCTCTCTTCACATCAGGCGGCAGCTGTCTCTGTTGAACCGGGCCAGCATTTC
AACCCTTCATTCGTTCTGCTTGCAGGTGCTCAGAAAATATTATTATCTCATTGATCTTGATCCGGGCTTTCGGATTGCCG
ATCAGACAGAAGGCGAGCTGCTCGGGGACGAGGTCCTTGATGAACTGTTTGAAGACGAATACGCGAAGGGTGAAAAAGCG
TTTTTCGAACTGGTGGACCGGTATACGACAGACAGGCACGACTTAGACCTTCAGCACCTCGTGAAACGGGTGTATGAGTT
TTCCCGTTCCCATCCCGATCCAAAAACGTGGCTGGAAGGTTTCGTTCATCTTTACGATGCCTCAGAAAAAAGCACGATTG
AAGAACTGCCGTTTTATCCATATATGAAAGAAGACATTGCGATGGTGCTGAGTGCTGTGAAAGAAAAGCTTCAGCGGGCG
CTTGAGCTGACAAAAGTGCCGGGCGGCCCTGCGCCGCGCGCTGATAATTTTCTGGATGATCTTGCACAGACAGAGGATCT
GATCGCACATTCGGCGGATTTCACTGAATTATACAAGCGGGTTCCCGCAGTCTCCTTTAAGCGTGCCAAAGCGGTCAAAG
GAGATGAATTTGACCAGGCGCTCACAGAAGAAGCGACGGATTTACGGAACGGCGCGAAAAAACTGCTGGAAAAACTAAAA
ACGGACTATTTCACAAGAAGCCCCGAGCAGCATCTGAAGAGCCTGTCGGATATGAAGCCTGTAATTGAAACGCTCGTCCA
GCTTGTCATCAGCTACGGAAAACGGTTTGAAGCCGCCAAACAAGAGAAATCCATTATTGATTTCTCTGACTTGGAGCATT
ACTGCTTGTCTATCCTGACAGCCGTGAATGAGTCGGGGGAGCGCAAGCCGAGTGAGGCGGCGGCTTACTATCAAGAGCAG
TTCCAAGAGGTGCTGGTTGACGAATATCAAGATACGAATCTTGTGCAGGAAGCCATACTGCAGCTTGTCACAAGCGGGCC
TGAAGCTTCAGGAAACTTGTTTATGGTAGGGGATGTCAAACAGTCCATCTACCGATTCAGGCTTGCCGAACCGCTTCTTT
TCCTTTCTAAATATAAACGATTTACCGAAACGGGAGAGGGGACAGGCCGGAAAATTGATTTGAACAAAAACTTTCGAAGC
CGGGCGGATATTTTAGACAGCACGAACTTTCTGTTTAAGCAGCTCATGGGCGGCAAGGTCGGCGAGGTTGATTATGATGA
GCAGGCGGAATTGAAGCTAGGAGCCTCTTACCCTCCGAATGACCAGACTGAAACGGAGCTGTTGCTGATTGACAGAGCGG
AGGAAAAAGAGGCGAGTGAAGAAACAGAAGAGCTCGAAACCGTTCAGCTTGAAGCAAAAGCGATTGCAAAGCAGATTCGA
AAACTCGTATCCTCTCCTTACAAAGTCTATGATGGAAAATCGAAAACACACAGCAATATCCGCTACCGCGATATCGTGAT
TTTACTGCGTTCCATGCCGTGGGCGCCGCAAATCATGGAAGAACTGAAAGCTCAAGGCATACCCGTCTATGCCAATTTGA
CATCAGGCTATTTTGAAGCGGTCGAGGTGTCTGTTGCGATTTCCGCTTTAAAAGTGATTGATAATCCGTACCAAGATATT
CCTCTCGCTTCCGTGCTGCGCTCGCCGATTGTCGGCGCAGATGAAAATGAACTTTCGCTCATTCGGATGCATAATAAAAA
AGCGCCTTTCTATGAAGCGATGAAGGACTATCTTGCAGGCGGGGACCGAAATGACGAGCTATATCAAAAGCTGAACACGT
TTTTTGAGCTTTTGCAAAAATGGCGCTCTTTTTCGAAAAACCATTCAGTTTCCGAGCTGATATGGGAAGTTTATCGTGAC
AGCAAATATATGGACTATGTCGGCGGTATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGTTCTCTATGACCGGTC
CCGCCAGTATGAATCGACGGCATTTCGCGGTTTATTTCGTTTCTTGCGGTTTATAGAACGTATGCAGGAACGGGGCGATG
ACCTCGGGACAGCGAGAGCGCTCAGCGAGCAGGAGGACGTGGTCCGCCTGATGACGATTCACAGCAGCAAAGGGCTTGAA
TTCCCTGTCGTATTCGCTGCGGGACTTGGACGGAATTTTAATATGATGGATTTGAATAAATCATATCTGCTGGACAAAGA
GCTTGGATTCGGCACAAAGTTTATTCACCCCGAATGGCGCATCAGCTATCCGACACTTCCGCTCATCGCCATGAAGAAAA
AAATGCGCCGGGAGCTGCTGTCAGAAGAGCTGCGTGTGCTATACGTGGCTTTAACAAGAGCGAAGGAAAAGCTGTTTCTT
GTCGGTTCCTGCAAGAATCGTGAGAAGCAGCTGGCGAAATGGCAGGCTTCAGCGTCTCAGCCAGACTGGCTTCTCCCGGA
TTTTGAGCGGTATCAGGCGAAAACGTATTTGGATTTCATCGGTCCCGCATTGATTCGTCACAGAGACATGGGGACAGATG
CTTTACAAGGGCTTGCGAGTCAAGCTGACATATCAGAACACCCTGCCCGGTTTAACATCCAAATGGTCAGCGCCCAAGAT
TTATTGGATGATGATCTGGAAGAAAGACAAGAGGAAAAGAGCGGCCGTCTTGACGCCATCCGCCGCGGTGAGCCTGTGTC
CGGTTCGTTTGAATTCGAAGAACAAGCGCGGCAACGCCTGTCATGGCGATACCCTTATCACGACGTGACAAAAATTCGCA
CGAAGCAGACTGTTTCCGAGATCAAGAGAAAGAAAGAATACGAGGACGAATACAGCGGCCGCTCTCTTATCAGACCTGCT
GACGGAAACATGTTGTACAGGCGCCCTGCGTTTATGATGAAAAAAGGGCTGACTGCGGCTGAAAAAGGAACGGCGATGCA
TACCGTTATGCAGCATATCCCGCTGACACATGCACCGTCAAAAGAGGAAGCGGAACGTATTGTCAAACGTCTTTATGAAA
AAGAGCTGCTCACCGAAGACCAGCAAGCCGTTATTGACCTAGAGGAAATCGTGCAATTTTTCCATACTGAAATCGGCGAA
AAGCTGATCGGCGCAAAATGGATTAACCGCGAAGTGCCGTTTAGCATGGCGCTTTCAGCACAAGATGCGTTTCTGGACAC
TGAATCTAAAGATGAGCCTATATTGGTGCAAGGAATTATTGACTGTCTGTATGAGACCGATGAAGGGCTTTTCTTGCTGG
ATTACAAAACGGACCGAATCGAAGGGAAATTCCAGCACGGATTTGAGGGGGCGGCTCCTATTCTTAAAAAACGGTACGAA
ACTCAAATCGAGCTATACACAAAAGCTGTAGAACAAATAACGAAAACCAATGTGAAGGGACGTGCACTCTACTTCTTCGA
CGGAGGACACGTTTTGAACGTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-881)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	86.548	100	0.865