Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACI8NS_RS04095 Genome accession   NZ_CP174125
Coordinates   768525..769139 (+) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain AX22     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 763525..774139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI8NS_RS04060 (ACI8NS_04060) aroE 764211..765053 (+) 843 WP_032873946.1 shikimate dehydrogenase -
  ACI8NS_RS04065 (ACI8NS_04065) yhbY 765047..765337 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  ACI8NS_RS04070 (ACI8NS_04070) - 765348..765917 (+) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  ACI8NS_RS04075 (ACI8NS_04075) yqeK 765907..766482 (+) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACI8NS_RS04080 (ACI8NS_04080) rsfS 766486..766842 (+) 357 WP_007408260.1 ribosome silencing factor -
  ACI8NS_RS04085 (ACI8NS_04085) - 766839..767576 (+) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  ACI8NS_RS04090 (ACI8NS_04090) comER 767645..768466 (-) 822 WP_012118027.1 late competence protein ComER -
  ACI8NS_RS04095 (ACI8NS_04095) comEA 768525..769139 (+) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACI8NS_RS04100 (ACI8NS_04100) - 769206..769775 (+) 570 WP_003152868.1 ComE operon protein 2 -
  ACI8NS_RS04105 (ACI8NS_04105) comEC 769776..772127 (+) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACI8NS_RS04110 (ACI8NS_04110) - 772146..772280 (-) 135 WP_003152870.1 YqzM family protein -
  ACI8NS_RS04115 (ACI8NS_04115) - 772321..772472 (+) 152 Protein_793 hypothetical protein -
  ACI8NS_RS04120 (ACI8NS_04120) holA 772512..773553 (+) 1042 Protein_794 DNA polymerase III subunit delta -
  ACI8NS_RS04125 (ACI8NS_04125) rpsT 773570..773836 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=1074443 ACI8NS_RS04095 WP_032873955.1 768525..769139(+) (comEA) [Bacillus velezensis strain AX22]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1074443 ACI8NS_RS04095 WP_032873955.1 768525..769139(+) (comEA) [Bacillus velezensis strain AX22]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363

Multiple sequence alignment