Detailed information NTG0107435

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AABA74_RS12580
• Genome accession: NZ_AP028932
• Coordinates: 2573703..2574317 (-)
• Length: 204 a.a.
• NCBI ID: WP_015387983.1
• Uniprot ID: A0A6A8LHQ3
• Organism: Bacillus velezensis strain RB.IBE29
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2568703..2579317

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AABA74_RS12550 (RBIBE_24170)	rpsT	2569003..2569269 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AABA74_RS12555 (RBIBE_24180)	holA	2569286..2570327 (-)	1042	Protein_2426	DNA polymerase III subunit delta	-
AABA74_RS12560	-	2570367..2570521 (-)	155	Protein_2427	hypothetical protein	-
AABA74_RS12565 (RBIBE_24190)	-	2570562..2570696 (+)	135	WP_003152870.1	YqzM family protein	-
AABA74_RS12570 (RBIBE_24200)	comEC	2570715..2573066 (-)	2352	WP_058906194.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AABA74_RS12575 (RBIBE_24210)	-	2573067..2573636 (-)	570	WP_003152868.1	ComE operon protein 2	-
AABA74_RS12580 (RBIBE_24220)	comEA	2573703..2574317 (-)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
AABA74_RS12585 (RBIBE_24230)	comER	2574376..2575197 (+)	822	WP_003152866.1	late competence protein ComER	-
AABA74_RS12590 (RBIBE_24240)	-	2575266..2576003 (-)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
AABA74_RS12595 (RBIBE_24250)	rsfS	2576000..2576356 (-)	357	WP_003152864.1	ribosome silencing factor	-
AABA74_RS12600 (RBIBE_24260)	yqeK	2576353..2576934 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AABA74_RS12605 (RBIBE_24270)	-	2576924..2577493 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
AABA74_RS12610 (RBIBE_24280)	yhbY	2577504..2577794 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AABA74_RS12615 (RBIBE_24290)	aroE	2577788..2578630 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22255.41 Da
• Isoelectric Point: 5.5211

>NTDB_id=107435 AABA74_RS12580 WP_015387983.1 2573703..2574317(-) (comEA) [Bacillus velezensis strain RB.IBE29]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=107435 AABA74_RS12580 WP_015387983.1 2573703..2574317(-) (comEA) [Bacillus velezensis strain RB.IBE29]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A6A8LHQ3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363