Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACI3EM_RS12225 Genome accession   NZ_CP174094
Coordinates   2492417..2493781 (-) Length   454 a.a.
NCBI ID   WP_020751747.1    Uniprot ID   -
Organism   Lacticaseibacillus paracasei strain Egylham_14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2487417..2498781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3EM_RS12205 - 2488406..2489182 (-) 777 WP_003591638.1 ABC transporter permease -
  ACI3EM_RS12210 - 2489200..2490087 (-) 888 Protein_2378 ABC transporter ATP-binding protein -
  ACI3EM_RS12215 - 2490271..2491167 (+) 897 WP_071252432.1 helix-turn-helix domain-containing protein -
  ACI3EM_RS12220 - 2491280..2492395 (-) 1116 WP_013246013.1 PIN/TRAM domain-containing protein -
  ACI3EM_RS12225 radA 2492417..2493781 (-) 1365 WP_020751747.1 DNA repair protein RadA Machinery gene
  ACI3EM_RS12230 - 2493798..2494340 (-) 543 WP_003567256.1 dUTP diphosphatase -
  ACI3EM_RS12235 - 2494561..2494851 (+) 291 WP_003595964.1 GNAT family N-acetyltransferase -
  ACI3EM_RS12240 - 2494968..2495345 (-) 378 WP_003567260.1 DUF805 domain-containing protein -
  ACI3EM_RS12245 - 2495590..2496909 (+) 1320 WP_003571381.1 C1 family peptidase -
  ACI3EM_RS12250 - 2497268..2498614 (+) 1347 WP_003576225.1 aminopeptidase C -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49142.47 Da        Isoelectric Point: 7.4216

>NTDB_id=1074246 ACI3EM_RS12225 WP_020751747.1 2492417..2493781(-) (radA) [Lacticaseibacillus paracasei strain Egylham_14]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSSKSVPRQTAAGSKVKPTRMNDVTITKETRVKTGLDELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLANTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEDVI
NQMQPDYVVIDSVQTMNVPEMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQDGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKSTGGVKLDEPAIDLAMAVAIASSYRDKEISPTDCFVGEIGLTGEV
RRVNRIEERVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1074246 ACI3EM_RS12225 WP_020751747.1 2492417..2493781(-) (radA) [Lacticaseibacillus paracasei strain Egylham_14]
ATGGCGAAAGCAAAAACACAATATGTTTGCCAAAACTGTGGCTATATTTCAGCCACTTATCTAGGCCGGTGTCCAAATTG
CGGCGGCTGGAACACCTTAGTGGAAGAAACCGTCAGTTCAAGCAAGTCTGTGCCGCGACAAACAGCAGCCGGCAGTAAGG
TAAAGCCGACGCGGATGAACGATGTGACGATCACCAAAGAAACGCGCGTCAAGACTGGCTTAGATGAACTGAACCGGGTG
CTTGGCGGCGGAGTGGTGCCAGGGTCACTGGTGCTGATCGGCGGGGATCCGGGGATTGGTAAATCAACCTTGCTATTACA
AGTATCAGGGCAGTTGGCTAATACCGGCGGAACCGTTTTGTATGTCTCTGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGCCGGCTTGGCGTGGCAAATTCGGGGATGTACTTGTATCCCGAAACCGACATGCCGAGTATTGAAGACGTCATC
AACCAAATGCAGCCGGATTACGTTGTGATCGACTCCGTTCAAACTATGAATGTGCCAGAAATGAATTCAGCGGTCGGTTC
TGTGGCACAGATTCGCGAAGTGACGGCAGAACTGATGCGCATTGCCAAGTCCAAAGGCGTGACCATTTTCATCGTTGGCC
ATGTCACTAAGGAAGGGGCGATTGCCGGGCCGAAGATTCTTGAGCATATGGTCGACACGGTGCTCTATTTTGAAGGCGAC
ATGCACCACACGTATCGAATCTTAAGATCCGTCAAAAATCGATTTGGATCGACCAATGAAATCGGCATTTTTGAAATGCA
TCAAGACGGGTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCTTGGAAGAACGCTTGGCGGGGGCAACCGGTTCTGCGG
TGGTTGTTTCGATGGAAGGCACCCGACCAATCTTAGTCGAGATTCAAGCCTTAATCAGTCCAACGATGTATGGCAATGCT
AAACGGACGAGCAGTGGGTTGGATCACAATCGCGTGAGCTTGATCATGGCCGTTCTTGAGAAGCGGGCTAACTTGATGTT
GCAAAATCAGGACGCCTATCTCAAGTCAACAGGCGGTGTGAAACTCGATGAGCCGGCGATTGACCTCGCCATGGCTGTGG
CAATTGCGTCATCTTATCGAGATAAGGAGATTTCACCGACAGATTGTTTTGTCGGTGAAATCGGATTGACGGGGGAAGTG
CGCCGCGTCAATCGGATCGAAGAACGTGTCAAAGAGGCGGCCAAACTGGGTTTCAAACGGATTTTTGTACCACGTAACAA
CTTGCAAGGCTGGCATGCGCCAAAAGATATTCAAGTCATTGGTGTTACAAGCATTGCCGAAGCGCTGCATAAGGTCTTTA
ATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-427)

AAA domain (AAA_25)

(69-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus pneumoniae Rx1

68.433

99.78

0.683
  radA Streptococcus pneumoniae D39

68.433

99.78

0.683
  radA Streptococcus pneumoniae R6

68.433

99.78

0.683
  radA Streptococcus pneumoniae TIGR4

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.212

99.78

0.681
  radA Bacillus subtilis subsp. subtilis str. 168

64.758

100

0.648

Multiple sequence alignment