Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACI5K2_RS11200 Genome accession   NZ_CP174089
Coordinates   2412176..2413540 (-) Length   454 a.a.
NCBI ID   WP_014570045.1    Uniprot ID   A0A7S7FQS2
Organism   Lacticaseibacillus rhamnosus strain 484     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2407176..2418540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI5K2_RS11185 (ACI5K2_11185) - 2408623..2409939 (-) 1317 WP_015765046.1 MucBP domain-containing protein -
  ACI5K2_RS11190 (ACI5K2_11190) - 2410238..2410948 (+) 711 Protein_2175 Rgg/GadR/MutR family transcriptional regulator -
  ACI5K2_RS11195 (ACI5K2_11195) - 2411040..2412155 (-) 1116 WP_005686113.1 PIN/TRAM domain-containing protein -
  ACI5K2_RS11200 (ACI5K2_11200) radA 2412176..2413540 (-) 1365 WP_014570045.1 DNA repair protein RadA Machinery gene
  ACI5K2_RS11205 (ACI5K2_11205) - 2413560..2414099 (-) 540 WP_005686110.1 dUTP diphosphatase -
  ACI5K2_RS11210 (ACI5K2_11210) - 2414740..2415030 (+) 291 WP_014570047.1 GNAT family N-acetyltransferase -
  ACI5K2_RS11215 (ACI5K2_11215) - 2415325..2416644 (+) 1320 WP_014570048.1 aminopeptidase C -
  ACI5K2_RS11220 (ACI5K2_11220) - 2416789..2418135 (+) 1347 WP_005714538.1 C1 family peptidase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49171.60 Da        Isoelectric Point: 8.3128

>NTDB_id=1074168 ACI5K2_RS11200 WP_014570045.1 2412176..2413540(-) (radA) [Lacticaseibacillus rhamnosus strain 484]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETISSTKSAPRQTRTGSKVKPTRMDEVTVTKETRVKTGLAELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLASTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIENVI
DQMQPDYVVIDSVQTMNVPDMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQNGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEIPPTDCFVGEIGLTGEV
RRVNRIEDRVKEAAKLGFKRIFVPRNNLQGWRAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1074168 ACI5K2_RS11200 WP_014570045.1 2412176..2413540(-) (radA) [Lacticaseibacillus rhamnosus strain 484]
ATGGCGAAAGCAAAAACACAATATGTATGTCAAAATTGCGGTTATATTTCGGCGACTTATTTAGGCCGTTGTCCAAACTG
CGGCGGCTGGAATACGCTCGTTGAAGAAACTATCAGTTCAACTAAATCGGCGCCGCGGCAAACGCGCACCGGCAGCAAGG
TTAAGCCCACACGCATGGATGAGGTCACGGTGACCAAAGAAACCCGGGTTAAAACAGGCTTGGCCGAGTTGAATCGCGTG
CTTGGTGGCGGTGTGGTTCCGGGCTCGTTGGTTCTGATTGGCGGCGATCCCGGGATTGGCAAATCAACCTTGCTGCTGCA
GGTGTCTGGCCAGCTAGCTAGCACAGGTGGTACGGTGTTGTACGTTTCAGGTGAAGAAAGCGCCAGCCAGATTAAAATGC
GGGCAGGGCGGCTAGGGGTTGCCAACTCCGGCATGTATTTGTATCCCGAAACCGACATGCCAAGTATCGAAAACGTGATT
GACCAGATGCAACCGGATTATGTGGTGATTGACTCGGTTCAAACCATGAATGTACCGGACATGAATTCGGCAGTCGGCTC
GGTTGCTCAGATTCGGGAAGTCACCGCCGAATTGATGCGAATTGCCAAGTCAAAAGGTGTGACGATTTTTATCGTTGGTC
ACGTGACGAAGGAAGGCGCTATTGCCGGGCCCAAGATTCTTGAGCATATGGTGGACACCGTGCTTTATTTTGAAGGCGAT
ATGCATCATACCTATCGCATCTTAAGGTCGGTCAAGAATCGGTTCGGCTCTACTAACGAAATTGGAATTTTTGAAATGCA
TCAAAATGGTTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCCTGGAAGAACGGCTGGCTGGTGCAACCGGATCGGCGG
TGGTGGTTTCGATGGAAGGCACTCGCCCGATTCTGGTTGAAATTCAGGCACTTATCAGCCCGACCATGTATGGCAATGCC
AAACGTACGAGCAGCGGCTTGGATCACAACCGCGTCAGCCTGATTATGGCCGTGCTGGAAAAACGTGCCAATTTGATGTT
GCAAAATCAGGACGCTTACTTGAAAGCAACAGGGGGCGTCAAGCTGGATGAACCGGCGATTGACTTGGCAATGGCAGTGG
CAATTGCCTCGTCATACCGCGACAAGGAAATCCCGCCGACTGATTGTTTTGTCGGTGAAATTGGCTTAACCGGTGAAGTG
CGGCGGGTGAATCGCATTGAAGACCGGGTGAAAGAGGCGGCTAAGTTAGGGTTTAAACGAATTTTTGTGCCGCGCAATAA
CCTGCAAGGCTGGCGTGCGCCTAAAGATATTCAGGTTATTGGCGTCACAAGCATTGCCGAAGCGCTGCATAAGGTTTTTA
ATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S7FQS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus pneumoniae Rx1

68.433

99.78

0.683
  radA Streptococcus pneumoniae D39

68.433

99.78

0.683
  radA Streptococcus pneumoniae R6

68.433

99.78

0.683
  radA Streptococcus pneumoniae TIGR4

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.212

99.78

0.681
  radA Bacillus subtilis subsp. subtilis str. 168

65.132

100

0.654

Multiple sequence alignment