Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   U3C44_RS19725 Genome accession   NZ_CP174016
Coordinates   4115312..4116745 (+) Length   477 a.a.
NCBI ID   WP_032659700.1    Uniprot ID   -
Organism   Enterobacter asburiae strain 2023CK-01510     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4110312..4121745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3C44_RS19695 (U3C44_19705) - 4110879..4111856 (+) 978 WP_023309354.1 calcium/sodium antiporter -
  U3C44_RS19700 (U3C44_19710) kdsD 4111870..4112856 (+) 987 WP_032659705.1 arabinose-5-phosphate isomerase KdsD -
  U3C44_RS19705 (U3C44_19715) kdsC 4112871..4113437 (+) 567 WP_032659704.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  U3C44_RS19710 (U3C44_19720) lptC 4113434..4114009 (+) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  U3C44_RS19715 (U3C44_19725) lptA 4113978..4114532 (+) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  U3C44_RS19720 (U3C44_19730) lptB 4114539..4115264 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  U3C44_RS19725 (U3C44_19735) rpoN/rpoN1 4115312..4116745 (+) 1434 WP_032659700.1 RNA polymerase factor sigma-54 Machinery gene
  U3C44_RS19730 (U3C44_19740) hpf 4116768..4117055 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  U3C44_RS19735 (U3C44_19745) ptsN 4117138..4117629 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  U3C44_RS19740 (U3C44_19750) rapZ 4117675..4118529 (+) 855 WP_014885397.1 RNase adapter RapZ -
  U3C44_RS19745 (U3C44_19755) npr 4118526..4118798 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  U3C44_RS19750 (U3C44_19760) mtgA 4118838..4119563 (-) 726 WP_029739613.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  U3C44_RS19755 (U3C44_19765) elbB 4119560..4120213 (-) 654 WP_032660483.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=1074096 U3C44_RS19725 WP_032659700.1 4115312..4116745(+) (rpoN/rpoN1) [Enterobacter asburiae strain 2023CK-01510]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1074096 U3C44_RS19725 WP_032659700.1 4115312..4116745(+) (rpoN/rpoN1) [Enterobacter asburiae strain 2023CK-01510]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGACGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACGCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCTAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTATGTGATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAACAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
AATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment