Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHOYE_RS20600 Genome accession   NZ_CP174015
Coordinates   4286013..4287446 (+) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter ludwigii strain 2024CK-01663     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4281013..4292446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHOYE_RS20570 (ACHOYE_20565) - 4281580..4282557 (+) 978 WP_020883528.1 calcium/sodium antiporter -
  ACHOYE_RS20575 (ACHOYE_20570) kdsD 4282571..4283557 (+) 987 WP_020883527.1 arabinose-5-phosphate isomerase KdsD -
  ACHOYE_RS20580 (ACHOYE_20575) kdsC 4283572..4284138 (+) 567 WP_063925622.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACHOYE_RS20585 (ACHOYE_20580) lptC 4284135..4284710 (+) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  ACHOYE_RS20590 (ACHOYE_20585) lptA 4284679..4285233 (+) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACHOYE_RS20595 (ACHOYE_20590) lptB 4285240..4285965 (+) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  ACHOYE_RS20600 (ACHOYE_20595) rpoN/rpoN1 4286013..4287446 (+) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  ACHOYE_RS20605 (ACHOYE_20600) hpf 4287469..4287756 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  ACHOYE_RS20610 (ACHOYE_20605) ptsN 4287839..4288330 (+) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACHOYE_RS20615 (ACHOYE_20610) rapZ 4288376..4289230 (+) 855 WP_014171820.1 RNase adapter RapZ -
  ACHOYE_RS20620 (ACHOYE_20615) npr 4289227..4289499 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  ACHOYE_RS20625 (ACHOYE_20620) mtgA 4289538..4290263 (-) 726 WP_063925623.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACHOYE_RS20630 (ACHOYE_20625) elbB 4290260..4290913 (-) 654 WP_032670144.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=1074074 ACHOYE_RS20600 WP_014171817.1 4286013..4287446(+) (rpoN/rpoN1) [Enterobacter ludwigii strain 2024CK-01663]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1074074 ACHOYE_RS20600 WP_014171817.1 4286013..4287446(+) (rpoN/rpoN1) [Enterobacter ludwigii strain 2024CK-01663]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGTTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATCGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATCCAACTTTCTCAGTTCAGCAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BTX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486

Multiple sequence alignment