Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AABA82_RS20605 Genome accession   NZ_AP028930
Coordinates   4640788..4642278 (-) Length   496 a.a.
NCBI ID   WP_039395518.1    Uniprot ID   A0A239S8W9
Organism   Pandoraea sputorum strain THI4931     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4635788..4647278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABA82_RS20575 (THI4931_40580) mutY 4635790..4636947 (+) 1158 WP_331692392.1 A/G-specific adenine glycosylase -
  AABA82_RS20580 (THI4931_40590) rapZ 4636964..4637836 (-) 873 WP_150661072.1 RNase adapter RapZ -
  AABA82_RS20585 (THI4931_40600) - 4638028..4638360 (-) 333 WP_338299128.1 PsiF family protein -
  AABA82_RS20590 (THI4931_40610) hprK 4638543..4639508 (-) 966 WP_039395509.1 HPr(Ser) kinase/phosphatase -
  AABA82_RS20595 (THI4931_40620) ptsN 4639599..4640054 (-) 456 WP_039395512.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AABA82_RS20600 (THI4931_40630) hpf 4640290..4640646 (-) 357 WP_039395515.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AABA82_RS20605 (THI4931_40640) rpoN/rpoN1 4640788..4642278 (-) 1491 WP_039395518.1 RNA polymerase factor sigma-54 Machinery gene
  AABA82_RS20610 (THI4931_40650) lptB 4642372..4643142 (-) 771 WP_039402130.1 LPS export ABC transporter ATP-binding protein -
  AABA82_RS20615 (THI4931_40660) lptA 4643177..4643869 (-) 693 WP_052252464.1 lipopolysaccharide transport periplasmic protein LptA -
  AABA82_RS20620 (THI4931_40670) lptC 4643866..4644543 (-) 678 WP_150661075.1 LPS export ABC transporter periplasmic protein LptC -
  AABA82_RS20625 (THI4931_40680) - 4644568..4645053 (-) 486 WP_322882185.1 KdsC family phosphatase -
  AABA82_RS20630 (THI4931_40690) - 4645175..4646155 (-) 981 WP_039395525.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55111.96 Da        Isoelectric Point: 5.1828

>NTDB_id=107380 AABA82_RS20605 WP_039395518.1 4640788..4642278(-) (rpoN/rpoN1) [Pandoraea sputorum strain THI4931]
MKPTLQLRTSQHLTLTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEREDDWLAGSVRVGTDGSLRNERSSSSGSSNDN
GPEASNGHDEARELDGEARFDDNASDNGSDWSLNDFARSGSASDDDDNARPQLHVDHPNLREHLLAQLRLTKASPRDRAL
VEFLIESLDEDGYLGSLLDEIQAEMPEELALETEEINAALALLQSFDPAGVGARSPAECLRLQLQRLPASGVRTLSLRIV
TDHLELLAARDYTRLRRLLGVGEDGLRAAHQLIRSLDPFPGAAYGAPQADYVVPDVLVRKQGSGWTAELNPEVMPRLRIN
EMYARILRNNRNDPGTGNLQQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHEST
ISRVTTSKYMLTPFGTFELKYFFGSHVATEAGGAASSTAIRALIKQLIGAEDPQSPLSDSRIAELLAEQGFVVARRTVAK
YREAMRIPAVNLRKSL

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=107380 AABA82_RS20605 WP_039395518.1 4640788..4642278(-) (rpoN/rpoN1) [Pandoraea sputorum strain THI4931]
ATGAAACCGACTCTTCAACTCCGCACCTCTCAGCACCTGACCCTCACGCCGCAGTTGCAGCAATCGATCCGGTTGTTGCA
ACTGTCGACGCTCGAACTTCAACAGGAAGTCGAGCACGCCCTTACCCAGAACCCGATGCTCGAACGCGAGGACGATTGGC
TCGCCGGTTCGGTACGTGTGGGCACAGACGGGTCGCTGCGCAACGAACGGAGTTCGTCGTCGGGCAGTTCGAACGACAAT
GGCCCCGAGGCCTCCAACGGTCACGACGAAGCGCGCGAACTCGATGGTGAGGCCCGCTTCGACGACAACGCGTCGGACAA
CGGCAGCGACTGGAGCCTGAACGACTTCGCCCGCTCCGGCAGCGCATCGGACGACGACGACAATGCGCGTCCGCAACTTC
ACGTCGACCACCCCAATCTCCGCGAACATCTGCTGGCCCAACTGCGCCTGACCAAGGCGAGTCCGCGCGACCGCGCGCTG
GTGGAATTCCTGATCGAATCGCTGGACGAAGACGGCTACCTCGGTAGCCTGCTCGACGAAATTCAGGCCGAAATGCCTGA
AGAACTGGCGCTCGAAACGGAAGAGATCAACGCGGCGCTGGCGCTGCTGCAAAGCTTCGACCCGGCGGGCGTGGGTGCCC
GCAGCCCGGCCGAATGCCTGCGTCTACAGCTCCAGCGCCTGCCCGCTAGCGGTGTGCGCACCCTGTCGCTGCGCATCGTC
ACCGATCACCTGGAACTGCTGGCCGCGCGCGATTACACGCGTCTGCGCCGCCTGCTAGGTGTCGGCGAAGACGGCCTGCG
CGCCGCGCATCAGCTGATTCGCTCGCTGGACCCCTTCCCGGGCGCGGCTTACGGCGCGCCTCAGGCCGACTACGTCGTGC
CGGACGTGCTGGTACGCAAGCAAGGGAGCGGCTGGACGGCGGAGCTGAATCCGGAAGTCATGCCGCGACTGCGTATCAAC
GAGATGTATGCGCGCATTCTCCGAAATAACCGGAACGATCCGGGAACCGGCAACCTCCAACAGCAGTTGCAGGAAGCGCG
CTGGCTGATCAAGAACATCCAGCAACGCTTCGACACGATCCTGCGGGTGGCGCAGGCGATTGTCGAGCGCCAAAAGAACT
TCTTCACGCATGGCGAAATCGGCATGCGGCCCTTGGTTTTACGCGAGATTGCTGATACGCTGGGTTTACATGAATCCACG
ATTTCACGCGTGACAACGAGTAAGTACATGCTCACTCCATTTGGAACCTTCGAGCTCAAGTACTTCTTCGGATCACACGT
GGCAACGGAAGCCGGAGGCGCTGCGTCATCGACCGCCATCCGCGCACTCATCAAGCAACTCATAGGAGCAGAAGACCCGC
AGAGCCCCCTTTCCGATAGCCGTATCGCGGAACTGCTGGCAGAGCAAGGTTTTGTGGTGGCGCGGCGCACTGTCGCGAAG
TACCGCGAGGCCATGCGAATCCCCGCAGTGAACTTGCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(134-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(339-494)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A239S8W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

69.062

100

0.698

Multiple sequence alignment