Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACI516_RS02435 Genome accession   NZ_CP173625
Coordinates   526822..528204 (-) Length   460 a.a.
NCBI ID   WP_039470544.1    Uniprot ID   A0A1M5J7R8
Organism   Pectobacterium carotovorum strain M8a     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 521822..533204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI516_RS02405 (ACI516_02400) pepP 522000..523325 (-) 1326 WP_039546943.1 Xaa-Pro aminopeptidase -
  ACI516_RS02410 (ACI516_02405) - 523400..523987 (-) 588 WP_010296270.1 YecA family protein -
  ACI516_RS02415 (ACI516_02410) zapA 524180..524509 (+) 330 WP_010284667.1 cell division protein ZapA -
  ACI516_RS02425 (ACI516_02420) - 524807..525454 (+) 648 WP_180775910.1 5-formyltetrahydrofolate cyclo-ligase -
  ACI516_RS02430 (ACI516_02425) nadR 525451..526704 (-) 1254 WP_119880571.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACI516_RS02435 (ACI516_02430) radA 526822..528204 (-) 1383 WP_039470544.1 DNA repair protein RadA Machinery gene
  ACI516_RS02440 (ACI516_02435) serB 528222..529199 (-) 978 WP_010296284.1 phosphoserine phosphatase -
  ACI516_RS02445 (ACI516_02440) - 529356..530054 (+) 699 WP_010296287.1 YtjB family periplasmic protein -
  ACI516_RS02450 (ACI516_02445) - 530109..531023 (-) 915 WP_231051828.1 hypothetical protein -
  ACI516_RS02455 (ACI516_02450) prfC 531317..532906 (+) 1590 WP_039546948.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49590.16 Da        Isoelectric Point: 7.4218

>NTDB_id=1073297 ACI516_RS02435 WP_039470544.1 526822..528204(-) (radA) [Pectobacterium carotovorum strain M8a]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASVSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKAPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1073297 ACI516_RS02435 WP_039470544.1 526822..528204(-) (radA) [Pectobacterium carotovorum strain M8a]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGACTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAATACCATTACCGAAGTCCGTCTGGCCTCGGCGTCTGTGTCACGTTCTGACCGTCTCACCGGCTATG
CGGGCGAAAGCGCTGGCGTCAGTCGGGTACAAAAGCTCTCAGAAATCAGTCTTGAAGCGCTGCCGCGCTTTTCTACTGGC
TTTCAGGAATTTGACCGCGTTCTGGGCGGCGGCGTCGTTCCGGGCAGCGCCATTCTGATCGGCGGTAACCCCGGCGCAGG
TAAAAGTACCCTGCTGCTGCAAACGCTCTGCAAGCTGTCAGAAAACATGAAAACCCTGTACGTCACCGGTGAAGAATCAC
TACAGCAGGTGGCAATGCGGGCACATCGCCTCAATTTGCCGACCCAAAATCTCAACATGCTGTCGGAAACCAGCATCGAA
CAGATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGCTGATGGTGATCGACTCCATTCAGGTCATGCATCTCGCCGATAT
TCAATCATCTCCCGGCAGCGTGGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTCGCCAAAACGCGCGGCGTTG
CTATCGTCATGGTCGGCCATGTCACCAAAGACGGTTCACTCGCCGGGCCAAAAGTCTTGGAACACTGTATCGACTGCTCC
GTGCTGCTGGATGGCGATGCCGATTCCCGCTTCCGCACGCTGCGCAGCCATAAAAACCGTTTCGGCGCCGTTAATGAGCT
GGGCGTGTTCGCGATGACGGAACAAGGTCTACGCGAGATCAGCAATCCGTCGGCGATTTTCCTCAGCCGCGGGGACGAAA
TCACGTCCGGCAGTTCGGTCATGGTAGTGTGGGAAGGCACACGTCCGCTGCTGGTCGAAATTCAGGCGCTGGTGGATCAG
TCGATGATGGCCAACCCGCGTCGCGTTGCAGTCGGGCTGGAGCAAAACCGATTAGCCATTCTGCTGGCGGTGCTGCATCG
ACACGGCGGCTTGCAGATGTCGGATCAGGATGTATTTGTGAATGTCGTCGGCGGCGTGAAAGTCACCGAAACCAGTGCCG
ACCTGGCGCTGCTGCTATCGCTGGTCTCCAGCTTCCGCGACCGCCCGCTGCCGCAGGATCTGGTCATCTTCGGTGAAGTC
GGTCTGGCGGGCGAAATCCGCCCGGTTCCCAGCGGACAAGAGCGGATTACCGAAGCCGCCAAGCACGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAGCCCCTGCCAGCATGCAGGTGTTCGGCGTGAAAAAGCTAGCCGACGCGC
TGGCAATCCTCGACGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1M5J7R8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.261

100

0.483
  radA Streptococcus mitis NCTC 12261

42.982

99.13

0.426
  radA Streptococcus mitis SK321

42.982

99.13

0.426
  radA Streptococcus pneumoniae Rx1

42.982

99.13

0.426
  radA Streptococcus pneumoniae D39

42.982

99.13

0.426
  radA Streptococcus pneumoniae R6

42.982

99.13

0.426
  radA Streptococcus pneumoniae TIGR4

42.982

99.13

0.426

Multiple sequence alignment