Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACI514_RS29560 Genome accession   NZ_CP173611
Coordinates   6621422..6622789 (+) Length   455 a.a.
NCBI ID   WP_007909773.1    Uniprot ID   J2YZH4
Organism   Pseudomonas sp. M20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 6616422..6627789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI514_RS29535 (ACI514_29520) - 6616682..6617395 (-) 714 WP_405122840.1 autoinducer binding domain-containing protein -
  ACI514_RS29540 (ACI514_29525) - 6617717..6618493 (+) 777 WP_160059495.1 ferredoxin--NADP reductase -
  ACI514_RS29545 (ACI514_29530) mscL 6618538..6618951 (-) 414 WP_034153825.1 large-conductance mechanosensitive channel protein MscL -
  ACI514_RS29550 (ACI514_29535) katB 6619238..6620779 (+) 1542 WP_160059494.1 catalase KatB -
  ACI514_RS29555 (ACI514_29540) - 6620840..6621376 (+) 537 WP_160059493.1 ankyrin repeat domain-containing protein -
  ACI514_RS29560 (ACI514_29545) radA 6621422..6622789 (+) 1368 WP_007909773.1 DNA repair protein RadA Machinery gene
  ACI514_RS29565 (ACI514_29550) - 6622910..6623278 (-) 369 WP_160059492.1 PilZ domain-containing protein -
  ACI514_RS29570 (ACI514_29555) - 6623438..6625504 (+) 2067 WP_160059491.1 carbon starvation CstA family protein -
  ACI514_RS29575 (ACI514_29560) - 6625520..6625717 (+) 198 WP_003228401.1 YbdD/YjiX family protein -
  ACI514_RS29580 (ACI514_29565) yjiA 6625881..6626852 (+) 972 WP_160059490.1 GTPase -
  ACI514_RS29585 (ACI514_29570) - 6626951..6627661 (-) 711 WP_405122841.1 GntR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48773.25 Da        Isoelectric Point: 6.8987

>NTDB_id=1073169 ACI514_RS29560 WP_007909773.1 6621422..6622789(+) (radA) [Pseudomonas sp. M20]
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLTETMIESGGATAPTGRTGWAGQQAQIKTLAEVSIEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLAKSMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARQEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKESPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1073169 ACI514_RS29560 WP_007909773.1 6621422..6622789(+) (radA) [Pseudomonas sp. M20]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGCGGCGCAACCTTTCCCAAGTGGGCTGGCCAGTGCGGTGAGTG
CGGTGCCTGGAATACCCTGACCGAGACCATGATCGAAAGTGGCGGCGCCACGGCACCGACCGGCCGTACCGGTTGGGCCG
GGCAACAGGCGCAGATCAAAACACTGGCCGAAGTCAGCATCGAAGAGATTCCGCGTTTCTCCACCGCGTCCGGTGAACTG
GACCGGGTCCTCGGTGGCGGTCTGGTCGACGGTTCGGTGGTGTTGATCGGCGGCGATCCAGGCATCGGCAAATCGACGAT
TCTGCTACAGACCCTGTGCAACCTCGCCAAAAGCATGCCGGCGCTCTACGTCACCGGCGAAGAATCCCAGCAACAAGTGG
CCATGCGCGCGCGCCGCCTCGGCTTGCCGCAGGACCAACTGCGGGTGATGACCGAAACCTGCATCGAAACCATCATCGCT
ACCGCCCGTCAGGAAAAGCCCAAGGTGATGGTGATCGACTCGATCCAGACGATCTTCACCGAACAACTGCAATCGGCGCC
CGGCGGCGTCTCCCAGGTGCGTGAGAGTGCAGCGCTGTTGGTGCGTTATGCCAAGCAGAGCGGCACGGCGATTTTCCTCG
TTGGCCACGTGACCAAGGAAGGTGCGCTGGCCGGCCCTCGCGTCTTGGAGCATATGGTCGACACCGTGCTGTATTTCGAA
GGTGAGTCCGATGGGCGTTTGCGTTTGCTGCGCGCGGTGAAAAACCGATTTGGTGCGGTCAATGAATTGGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTCTCCAACCCGTCGGCGATTTTTCTCACTCGTGCGCAGGAAGAAGTCCCGGGCA
GTGTGGTCATGGCGACGTGGGAAGGCACGCGGCCGATGCTGGTGGAAGTGCAGGCCTTGGTCGATGACAGTCATCTGGCC
AACCCGCGTCGGGTGACGCTGGGGCTGGATCAGAACCGTTTGGCGATGTTGCTCGCAGTTTTGCATCGCCATGGCGGCAT
CCCGACTCATGATCAGGATGTGTTCCTCAACGTGGTGGGCGGGGTGAAGGTGCTGGAAACGGCGTCTGACCTGGCGTTGA
TGGCGGCGGTGATGTCGAGTTTGCGCAATCGACCGTTGCCGCATGATCTGTTGGTGTTCGGCGAGGTGGGCTTGTCCGGT
GAAGTGCGCCCGGTGCCGAGCGGGCAGGAGCGATTGAAGGAAGCGGCCAAACATGGCTTCAAGCGAGCTATTGTGCCGAA
GGGCAATGCGCCGAAGGAATCCCCTCCGGGTTTGCAGATTATTGCCGTGACCCGCCTCGAACAGGCCCTCGACGCCCTCT
TCGAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(81-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J2YZH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.453

100

0.497
  radA Streptococcus mitis SK321

46.491

100

0.466
  radA Streptococcus mitis NCTC 12261

46.272

100

0.464
  radA Streptococcus pneumoniae Rx1

46.272

100

0.464
  radA Streptococcus pneumoniae D39

46.272

100

0.464
  radA Streptococcus pneumoniae R6

46.272

100

0.464
  radA Streptococcus pneumoniae TIGR4

46.272

100

0.464

Multiple sequence alignment