Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACI2O8_RS13355 Genome accession   NZ_CP173415
Coordinates   2564552..2564962 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain FJYA24     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2559552..2569962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI2O8_RS13325 (ACI2O8_13325) - 2559663..2559803 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ACI2O8_RS13330 (ACI2O8_13330) yqeF 2560109..2560840 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  ACI2O8_RS13335 (ACI2O8_13335) cwlH 2561092..2561844 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ACI2O8_RS13340 (ACI2O8_13340) yqeD 2562031..2562658 (+) 628 Protein_2579 TVP38/TMEM64 family protein -
  ACI2O8_RS13345 (ACI2O8_13345) gnd 2562677..2563545 (-) 869 Protein_2580 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACI2O8_RS13350 (ACI2O8_13350) yqeB 2563797..2564519 (+) 723 WP_010886572.1 hypothetical protein -
  ACI2O8_RS13355 (ACI2O8_13355) nucA/comI 2564552..2564962 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ACI2O8_RS13360 (ACI2O8_13360) - 2565158..2565508 (+) 351 Protein_2583 sigma-70 family RNA polymerase sigma factor -
  ACI2O8_RS13365 (ACI2O8_13365) spoIVCA 2565536..2566996 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  ACI2O8_RS13370 (ACI2O8_13370) - 2566954..2567132 (-) 179 Protein_2585 hypothetical protein -
  ACI2O8_RS13375 (ACI2O8_13375) arsC 2567487..2567906 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  ACI2O8_RS13380 (ACI2O8_13380) acr3 2567918..2568958 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  ACI2O8_RS13385 (ACI2O8_13385) arsK 2568981..2569421 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  ACI2O8_RS13390 (ACI2O8_13390) arsR 2569482..2569799 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1072115 ACI2O8_RS13355 WP_009967785.1 2564552..2564962(-) (nucA/comI) [Bacillus subtilis strain FJYA24]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1072115 ACI2O8_RS13355 WP_009967785.1 2564552..2564962(-) (nucA/comI) [Bacillus subtilis strain FJYA24]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment