Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACI3L7_RS02125 Genome accession   NZ_CP173387
Coordinates   455151..456167 (-) Length   338 a.a.
NCBI ID   WP_005432712.1    Uniprot ID   -
Organism   Vibrio campbellii strain LB135     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450151..461167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L7_RS02100 (ACI3L7_02100) aroK 451017..451535 (-) 519 WP_010443904.1 shikimate kinase AroK -
  ACI3L7_RS02105 (ACI3L7_02105) pilQ 451724..453466 (-) 1743 WP_050906101.1 type IV pilus secretin PilQ Machinery gene
  ACI3L7_RS02110 (ACI3L7_02110) pilP 453501..454016 (-) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  ACI3L7_RS02115 (ACI3L7_02115) pilO 454006..454599 (-) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACI3L7_RS02120 (ACI3L7_02120) pilN 454592..455167 (-) 576 WP_404970175.1 PilN domain-containing protein Machinery gene
  ACI3L7_RS02125 (ACI3L7_02125) pilM 455151..456167 (-) 1017 WP_005432712.1 type IV pilus biogenesis protein PilM Machinery gene
  ACI3L7_RS02130 (ACI3L7_02130) - 456346..458913 (+) 2568 WP_038889585.1 penicillin-binding protein 1A -
  ACI3L7_RS02135 (ACI3L7_02135) oxyR 458992..459900 (-) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  ACI3L7_RS02140 (ACI3L7_02140) - 460075..460803 (+) 729 WP_005432732.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37860.29 Da        Isoelectric Point: 6.3141

>NTDB_id=1071916 ACI3L7_RS02125 WP_005432712.1 455151..456167(-) (pilM) [Vibrio campbellii strain LB135]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1071916 ACI3L7_RS02125 WP_005432712.1 455151..456167(-) (pilM) [Vibrio campbellii strain LB135]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGGGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAGGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAATCTCCATTCCCTGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACCCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACTGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAAGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

98.225

100

0.982
  pilM Vibrio cholerae strain A1552

49.415

100

0.5

Multiple sequence alignment