Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAA995_RS00245 Genome accession   NZ_AP028877
Coordinates   38911..40272 (+) Length   453 a.a.
NCBI ID   WP_078139281.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 21P20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 33911..45272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA995_RS00205 - 33958..34167 (+) 210 Protein_34 transposase -
  AAA995_RS00210 - 34202..35063 (-) 862 Protein_35 transposase -
  AAA995_RS00215 (SP21P20_00400) comW 35329..35565 (+) 237 WP_000939545.1 sigma(X)-activator ComW Regulator
  AAA995_RS00220 (SP21P20_00410) - 35796..37082 (+) 1287 WP_000205044.1 adenylosuccinate synthase -
  AAA995_RS00225 (SP21P20_00420) tadA 37283..37750 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  AAA995_RS00235 (SP21P20_00430) - 37937..38380 (+) 444 WP_000701992.1 dUTP diphosphatase -
  AAA995_RS00240 (SP21P20_00440) - 38382..38897 (+) 516 WP_000691236.1 histidine phosphatase family protein -
  AAA995_RS00245 (SP21P20_00450) radA 38911..40272 (+) 1362 WP_078139281.1 DNA repair protein RadA Machinery gene
  AAA995_RS00250 (SP21P20_00460) - 40345..40842 (+) 498 WP_001809263.1 carbonic anhydrase -
  AAA995_RS00255 (SP21P20_00470) - 40867..41682 (+) 816 WP_000749763.1 PrsW family intramembrane metalloprotease -
  AAA995_RS00260 (SP21P20_00480) - 41827..42795 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  AAA995_RS00265 - 42929..43210 (-) 282 Protein_45 ISL3 family transposase -
  AAA995_RS00270 - 43337..44244 (-) 908 Protein_46 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49420.89 Da        Isoelectric Point: 6.5137

>NTDB_id=107168 AAA995_RS00245 WP_078139281.1 38911..40272(+) (radA) [Streptococcus pneumoniae strain 21P20]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSTNVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=107168 AAA995_RS00245 WP_078139281.1 38911..40272(+) (radA) [Streptococcus pneumoniae strain 21P20]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAAGTTAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCACCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTTATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCGATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(61-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA Bacillus subtilis subsp. subtilis str. 168

62.693

100

0.627

Multiple sequence alignment