Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACIQTD_RS11140 Genome accession   NZ_CP173185
Coordinates   2296282..2297643 (-) Length   453 a.a.
NCBI ID   WP_404399175.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain MGEL24009     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2291282..2302643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIQTD_RS11105 - 2291829..2292323 (+) 495 WP_012898594.1 GNAT family N-acetyltransferase -
  ACIQTD_RS11110 - 2292415..2292738 (-) 324 WP_017865144.1 DUF1048 domain-containing protein -
  ACIQTD_RS11115 - 2292725..2293072 (-) 348 WP_058204407.1 DUF1048 domain-containing protein -
  ACIQTD_RS11120 - 2293059..2293421 (-) 363 WP_003129889.1 PadR family transcriptional regulator -
  ACIQTD_RS11125 - 2293542..2294171 (-) 630 WP_017865146.1 hypothetical protein -
  ACIQTD_RS11130 - 2294236..2295321 (-) 1086 WP_012898597.1 PIN/TRAM domain-containing protein -
  ACIQTD_RS11135 - 2295552..2296238 (+) 687 WP_012898598.1 YoaK family protein -
  ACIQTD_RS11140 radA 2296282..2297643 (-) 1362 WP_404399175.1 DNA repair protein RadA Machinery gene
  ACIQTD_RS11145 - 2297805..2298032 (-) 228 WP_058219032.1 heavy-metal-associated domain-containing protein -
  ACIQTD_RS11155 rplQ 2298750..2299130 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  ACIQTD_RS11160 - 2299149..2300087 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  ACIQTD_RS11165 rpsK 2300135..2300518 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  ACIQTD_RS11170 rpsM 2300539..2300904 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  ACIQTD_RS11175 rpmJ 2300922..2301038 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  ACIQTD_RS11180 infA 2301092..2301310 (-) 219 WP_003130554.1 translation initiation factor IF-1 -
  ACIQTD_RS11185 - 2301607..2302254 (-) 648 WP_010906298.1 adenylate kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49907.31 Da        Isoelectric Point: 5.1777

>NTDB_id=1070513 ACIQTD_RS11140 WP_404399175.1 2296282..2297643(-) (radA) [Lactococcus lactis subsp. lactis strain MGEL24009]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDREFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1070513 ACIQTD_RS11140 WP_404399175.1 2296282..2297643(-) (radA) [Lactococcus lactis subsp. lactis strain MGEL24009]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCTTTGGTTCTGATTGGGGGAGATCCTGGGATAGGTAAATCAACTTTACTTTTACA
GGTTTCCACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGAATTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGGCCGAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
ACATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGTTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCAAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTGACAACTTTAACTGAATGTTTAAAACTGGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(70-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.457

100

0.815
  radA Streptococcus pneumoniae D39

81.457

100

0.815
  radA Streptococcus pneumoniae R6

81.457

100

0.815
  radA Streptococcus pneumoniae TIGR4

81.457

100

0.815
  radA Streptococcus mitis SK321

81.457

100

0.815
  radA Streptococcus mitis NCTC 12261

81.236

100

0.812
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62

Multiple sequence alignment