Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACIHQS_RS02140 Genome accession   NZ_CP173025
Coordinates   435435..436235 (+) Length   266 a.a.
NCBI ID   WP_007482693.1    Uniprot ID   -
Organism   Acinetobacter venetianus strain AVYS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430435..441235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIHQS_RS02115 (ACIHQS_02115) - 431332..432648 (+) 1317 WP_404230128.1 glycosyltransferase family 4 protein -
  ACIHQS_RS02120 (ACIHQS_02120) - 432674..433225 (+) 552 WP_404230131.1 phosphatase PAP2 family protein -
  ACIHQS_RS02125 (ACIHQS_02125) iscX 433322..433522 (+) 201 WP_004877416.1 Fe-S cluster assembly protein IscX -
  ACIHQS_RS02130 (ACIHQS_02130) ndk 433631..434062 (+) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  ACIHQS_RS02135 (ACIHQS_02135) rlmN 434183..435418 (+) 1236 WP_004877414.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACIHQS_RS02140 (ACIHQS_02140) pilF 435435..436235 (+) 801 WP_007482693.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACIHQS_RS02145 (ACIHQS_02145) - 436226..437017 (+) 792 WP_004877405.1 helix-turn-helix domain-containing protein -
  ACIHQS_RS02150 (ACIHQS_02150) ispG 437040..438155 (+) 1116 WP_004877403.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACIHQS_RS02155 (ACIHQS_02155) hisS 438152..439444 (+) 1293 WP_278596506.1 histidine--tRNA ligase -
  ACIHQS_RS02160 (ACIHQS_02160) - 439476..440174 (+) 699 WP_019383701.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30300.28 Da        Isoelectric Point: 9.4398

>NTDB_id=1069991 ACIHQS_RS02140 WP_007482693.1 435435..436235(+) (pilF) [Acinetobacter venetianus strain AVYS1]
MNTTRSKTIIATTILFTVLGLTACQSTKTTKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDSRDANANMMMGIL
LQQEGSKTNMEKAESYFKRAISSEPDNAQARNNYGTYLYQMERYNEAIEQFTIAGATLGYDQRFQSLENLGRIYLKLGDV
ANAEKAFKQALQANRNATISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGLRIARANADQLGMQVLVNQ
LRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1069991 ACIHQS_RS02140 WP_007482693.1 435435..436235(+) (pilF) [Acinetobacter venetianus strain AVYS1]
TTGAATACAACTCGATCAAAAACGATTATAGCAACTACTATTTTGTTCACTGTATTGGGTCTAACCGCCTGCCAATCGAC
AAAGACAACAAAAGATCCTGAAAAAGCTGTGCAAGTTCGAACACAACTTGCAGCAGAATATATTCGAACGCGTGACTTAG
ACTCGGCTAAGCGATCATTAGATCAAGCCCTGAAAATAGATTCGCGAGATGCAAATGCCAATATGATGATGGGAATTTTG
CTACAACAAGAAGGTAGCAAAACCAATATGGAAAAAGCAGAGTCTTATTTTAAGCGTGCAATATCTTCTGAACCTGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGGAGCGTTATAATGAGGCGATTGAGCAGTTTACGATTG
CAGGTGCAACCTTAGGTTATGATCAACGTTTTCAATCGTTGGAAAATCTTGGGCGAATCTATTTAAAACTGGGCGATGTT
GCGAATGCTGAAAAAGCATTCAAACAAGCGCTACAAGCAAATCGAAATGCGACAATATCAATGTTAGAGTTGTCGGAAAT
TTTCTATTTGCAACAAAACAATAGAGATGCATCGCAACTCTACGAACAATATGTTCGTAATGTGGGGCAGAAAAACCAAG
GTGCTCGCGCGCTTTGGATAGGTTTACGCATTGCTCGAGCGAATGCGGATCAGTTAGGAATGCAAGTGTTAGTCAATCAG
TTGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-132)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.441

98.872

0.677

Multiple sequence alignment