Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RSP597_RS08170 Genome accession   NZ_CP172969
Coordinates   1705733..1707232 (+) Length   499 a.a.
NCBI ID   WP_013207040.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain P597     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1700733..1712232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP597_RS08145 (RSP597_008145) - 1701912..1702895 (+) 984 WP_003269858.1 SIS domain-containing protein -
  RSP597_RS08150 (RSP597_008150) - 1702928..1703515 (+) 588 WP_064045771.1 HAD family hydrolase -
  RSP597_RS08155 (RSP597_008155) lptC 1703517..1704149 (+) 633 WP_013207037.1 LPS export ABC transporter periplasmic protein LptC -
  RSP597_RS08160 (RSP597_008160) lptA 1704217..1704813 (+) 597 WP_013207038.1 lipopolysaccharide transport periplasmic protein LptA -
  RSP597_RS08165 (RSP597_008165) lptB 1704847..1705635 (+) 789 WP_003262277.1 LPS export ABC transporter ATP-binding protein -
  RSP597_RS08170 (RSP597_008170) rpoN/rpoN1 1705733..1707232 (+) 1500 WP_013207040.1 RNA polymerase factor sigma-54 Machinery gene
  RSP597_RS08175 (RSP597_008175) hpf 1707284..1707637 (+) 354 WP_003269867.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RSP597_RS08180 (RSP597_008180) ptsN 1707987..1708442 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RSP597_RS08185 (RSP597_008185) hprK 1708513..1709487 (+) 975 WP_003262273.1 HPr(Ser) kinase/phosphatase -
  RSP597_RS08190 (RSP597_008190) - 1709678..1710004 (+) 327 WP_064045772.1 PsiF family protein -
  RSP597_RS08195 (RSP597_008195) rapZ 1710098..1710991 (+) 894 WP_013207042.1 RNase adapter RapZ -
  RSP597_RS08200 (RSP597_008200) - 1711069..1711722 (+) 654 WP_003269875.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55202.61 Da        Isoelectric Point: 4.8496

>NTDB_id=1069768 RSP597_RS08170 WP_013207040.1 1705733..1707232(+) (rpoN/rpoN1) [Ralstonia solanacearum strain P597]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSNSATPLPTE
SPPPAANGSDHADTAEAPYENGSDFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTMREFLMEQLAPLKLSTR
DKGLVIFLIESLDDEGYLTGSLDEILTDLPAELEIELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1069768 RSP597_RS08170 WP_013207040.1 1705733..1707232(+) (rpoN/rpoN1) [Ralstonia solanacearum strain P597]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTGGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGATGGCTCGGTCAGCGCACTGCAGAGCAACAGCGCCACCCCGCTGCCGACCGAG
TCGCCGCCGCCCGCCGCCAACGGCTCGGACCACGCCGACACGGCCGAGGCACCGTACGAAAACGGCAGCGACTTCGACAG
CGACTACGGCTCCGATCGCTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCCCAGTCCGACGAAGACGATCGCGCTC
CGATGCAGTTGCGCGAGGCCGAGCCCACGATGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCACGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAGGGCTACCTGACCGGCTCGCTCGACGAGATCCTCAC
CGACCTGCCGGCCGAACTCGAGATCGAACTCGACGAACTGCAGGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGGCCG
GCATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTCGCGCTG
GCCATCGTCAACCAGCATCTCGAATTGCTCGCCGCGCGCGACTACACGCGCCTGAAGAAAGCGCTGTCCGTCGACGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCACCGTTCCCGGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TCGTCGTGCCCGATGTCGTGGTGCGTAAAACCGCCGCAGGCTGGATGGCGCAACTGAACCCGGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCCCAGATCCTGCGCAGCAGCCGCGGCGAGTCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
AGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCAGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974

Multiple sequence alignment