Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   AADG46_RS10905 Genome accession   NZ_AP028804
Coordinates   2392955..2393539 (-) Length   194 a.a.
NCBI ID   WP_000902746.1    Uniprot ID   -
Organism   Vibrio cholerae strain VN152     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2387955..2398539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADG46_RS10880 (VNVC001_20640) aroB 2388293..2389378 (-) 1086 WP_000439803.1 3-dehydroquinate synthase -
  AADG46_RS10885 (VNVC001_20650) aroK 2389406..2389930 (-) 525 WP_000818616.1 shikimate kinase AroK -
  AADG46_RS10890 (VNVC001_20660) pilQ 2390117..2391838 (-) 1722 WP_162797396.1 type IV pilus secretin PilQ family protein Machinery gene
  AADG46_RS10895 (VNVC001_20670) pilP 2391864..2392382 (-) 519 WP_138041023.1 pilus assembly protein PilP Machinery gene
  AADG46_RS10900 (VNVC001_20680) pilO 2392372..2392962 (-) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  AADG46_RS10905 (VNVC001_20690) pilN 2392955..2393539 (-) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  AADG46_RS10910 (VNVC001_20700) pilM 2393529..2394545 (-) 1017 WP_172780299.1 type IV pilus assembly protein PilM Machinery gene
  AADG46_RS10915 (VNVC001_20710) - 2394688..2397165 (+) 2478 WP_002044688.1 penicillin-binding protein 1A -
  AADG46_RS10920 (VNVC001_20720) oxyR 2397240..2398133 (-) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22022.45 Da        Isoelectric Point: 9.7294

>NTDB_id=106924 AADG46_RS10905 WP_000902746.1 2392955..2393539(-) (pilN) [Vibrio cholerae strain VN152]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=106924 AADG46_RS10905 WP_000902746.1 2392955..2393539(-) (pilN) [Vibrio cholerae strain VN152]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCCGGTGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATTTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTTATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACATAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

99.485

100

0.995
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552

Multiple sequence alignment