Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACAM43_RS30505 Genome accession   NZ_AP028671
Coordinates   6569753..6571342 (+) Length   529 a.a.
NCBI ID   WP_354347981.1    Uniprot ID   -
Organism   Variovorax sp. V77     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6564753..6576342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM43_RS30480 (GmRootV77_60390) argS 6564832..6566517 (+) 1686 WP_369631028.1 arginine--tRNA ligase -
  ACAM43_RS30485 (GmRootV77_60400) - 6566532..6567302 (+) 771 WP_070063732.1 SPOR domain-containing protein -
  ACAM43_RS30490 (GmRootV77_60410) - 6567459..6568103 (+) 645 WP_062483056.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACAM43_RS30495 (GmRootV77_60420) lptA 6568225..6568944 (+) 720 WP_369631029.1 lipopolysaccharide transport periplasmic protein LptA -
  ACAM43_RS30500 (GmRootV77_60430) lptB 6568941..6569735 (+) 795 WP_369631030.1 LPS export ABC transporter ATP-binding protein -
  ACAM43_RS30505 (GmRootV77_60440) rpoN/rpoN1 6569753..6571342 (+) 1590 WP_354347981.1 RNA polymerase factor sigma-54 Machinery gene
  ACAM43_RS30510 (GmRootV77_60450) - 6571353..6572969 (+) 1617 WP_369631031.1 phospholipase D family protein -
  ACAM43_RS30515 (GmRootV77_60460) - 6572974..6573621 (-) 648 WP_307587626.1 LysE family translocator -
  ACAM43_RS30520 (GmRootV77_60470) - 6573647..6574279 (-) 633 WP_307587789.1 YceI family protein -
  ACAM43_RS30525 (GmRootV77_60480) - 6574318..6574902 (-) 585 WP_095949693.1 YceI family protein -
  ACAM43_RS30530 (GmRootV77_60490) - 6574899..6575510 (-) 612 WP_369631032.1 cytochrome b -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58368.65 Da        Isoelectric Point: 4.6825

>NTDB_id=106853 ACAM43_RS30505 WP_354347981.1 6569753..6571342(+) (rpoN/rpoN1) [Variovorax sp. V77]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDTADAPVPRDDAGEGEAEF
TPSAASTSSTSSDTTTSTPDTDGPAAEIAEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESLQ
SFLHRQALSLRLNENDCAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPVGVGARDLG
ECLSIQLKALASEDETEAEALIRKTAIAICKQPMELLARRDFKRLATLTRTNEDLVRSALQVIARLEPKPGRRFVDVERN
IVIPDVIVTKTGRGTNVKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=106853 ACAM43_RS30505 WP_354347981.1 6569753..6571342(+) (rpoN/rpoN1) [Variovorax sp. V77]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCATCTGGCCTTGACGCCTCAGCTGCAGCAGTCGATCCGGCTTCTGCA
GCTCTCCACGCTCGAACTGAGCCAGGAGGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAGCGCACCGCGGAAGAAG
CGGCGCGCGAGGAATTCGGGCTGGACACGGCCGACGCGCCGGTGCCGCGCGACGACGCGGGCGAGGGCGAGGCCGAGTTC
ACGCCGTCCGCCGCCAGCACGTCCTCCACCTCCAGCGACACCACCACCTCGACCCCCGACACCGACGGCCCCGCCGCCGA
GATCGCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAGTGGGGCAGCGACG
CGCCCGCGCGCCAGAACAACCTGGGCGACGACGAGCGTGCCGACGCCACCGAACTCGCGCGCAGCCAGGAGTCGCTGCAG
TCGTTCCTGCACCGCCAGGCGCTGAGCCTGCGGCTCAATGAAAACGACTGCGCCGCGCTGCGCTTCCTGATCGAGTCGCT
CAACGACGACGGCTACCTCGAAGACTCGCTGCCCGCATTGGCCTCGGGCCTGGCGGGCGACGACAACGACCAGTTCGACG
ACCTCGTGCACCACTTCCAGGTGGCGCTGGGCCTGCTGCAGAGCCTGGAGCCGGTGGGCGTCGGCGCGCGCGACCTCGGC
GAATGCCTGAGCATCCAGCTGAAGGCGCTTGCCAGCGAAGACGAGACCGAGGCCGAAGCGCTCATCCGCAAGACCGCCAT
CGCGATCTGCAAGCAGCCCATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGCACCAACGAAG
ACCTGGTGCGCTCGGCGCTGCAGGTCATTGCGCGGCTCGAGCCCAAGCCGGGGCGCCGCTTTGTCGACGTCGAACGCAAC
ATCGTCATTCCCGACGTGATCGTCACCAAGACCGGGCGCGGCACCAACGTGAAGTTCCGCGTCATGCTCAACCCCGAGGT
CATGCCGCGCCTGCGCGTGCACGACATTTACGCCGGTGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGTCGC
AGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCCATC
GTCGAGCGCCAGAAGAGCTACTTCGTGCACGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
GGGCCTGCACGAGTCGACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACGGTCGAGCTGAAGT
ACTTCTTCGGTTCGGCGCTGGGCACCGAGACCGGTGGCAATGCGTCGAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTC
GTGAGTTCCGAGAGCATCAAGAAGCCGCTGTCCGACAGCCAGATCTCCGAGATGCTGAAAGAGCAGGGCATCGAGTGCGC
GCGCCGCACCGTCGCCAAGTACCGCGAAGCGCTGCGCATCGCACCGGCCAACCTGCGCAAGGCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.601

100

0.518

Multiple sequence alignment