Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFVP7_RS02035 Genome accession   NZ_CP172606
Coordinates   379977..380426 (-) Length   149 a.a.
NCBI ID   WP_070548532.1    Uniprot ID   -
Organism   Bacillus sp. SG20033     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 374977..385426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFVP7_RS01995 (ACFVP7_01995) - 375299..375496 (+) 198 WP_122060560.1 hypothetical protein -
  ACFVP7_RS02000 (ACFVP7_02000) - 375516..375971 (+) 456 WP_396582940.1 RDD family protein -
  ACFVP7_RS02005 (ACFVP7_02005) - 376052..376384 (+) 333 WP_021481829.1 YckD family protein -
  ACFVP7_RS02010 (ACFVP7_02010) - 376511..376933 (+) 423 WP_170939093.1 MarR family winged helix-turn-helix transcriptional regulator -
  ACFVP7_RS02015 (ACFVP7_02015) - 376921..377262 (+) 342 WP_032724911.1 DUF3147 family protein -
  ACFVP7_RS02020 (ACFVP7_02020) - 377281..378009 (+) 729 WP_021481826.1 AAA family ATPase -
  ACFVP7_RS02025 (ACFVP7_02025) bglC 378082..379515 (+) 1434 WP_123373936.1 6-phospho-beta-glucosidase -
  ACFVP7_RS02030 (ACFVP7_02030) nin/comJ 379552..379950 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  ACFVP7_RS02035 (ACFVP7_02035) nucA/comI 379977..380426 (-) 450 WP_070548532.1 DNA-entry nuclease Machinery gene
  ACFVP7_RS02040 (ACFVP7_02040) tlpC 380594..382315 (-) 1722 WP_396582941.1 methyl-accepting chemotaxis protein TlpC -
  ACFVP7_RS02045 (ACFVP7_02045) hxlB 382426..382983 (-) 558 WP_021481820.1 6-phospho-3-hexuloisomerase -
  ACFVP7_RS02050 (ACFVP7_02050) hxlA 382989..383621 (-) 633 WP_021481819.1 3-hexulose-6-phosphate synthase -
  ACFVP7_RS02055 (ACFVP7_02055) hxlR 383853..384215 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.35 Da        Isoelectric Point: 4.4397

>NTDB_id=1068270 ACFVP7_RS02035 WP_070548532.1 379977..380426(-) (nucA/comI) [Bacillus sp. SG20033]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLQDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1068270 ACFVP7_RS02035 WP_070548532.1 379977..380426(-) (nucA/comI) [Bacillus sp. SG20033]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTACAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment