Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFMPA_RS13045 Genome accession   NZ_CP172605
Coordinates   2542714..2543124 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus sp. SG20032     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2537714..2548124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFMPA_RS13015 (ACFMPA_13015) - 2537801..2537941 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ACFMPA_RS13020 (ACFMPA_13020) - 2538247..2538978 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ACFMPA_RS13025 (ACFMPA_13025) cwlH 2539230..2539982 (-) 753 WP_088326549.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ACFMPA_RS13030 (ACFMPA_13030) - 2540169..2540795 (+) 627 WP_116316520.1 TVP38/TMEM64 family protein -
  ACFMPA_RS13035 (ACFMPA_13035) gnd 2540814..2541707 (-) 894 WP_151262339.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACFMPA_RS13040 (ACFMPA_13040) - 2541959..2542681 (+) 723 WP_010886572.1 hypothetical protein -
  ACFMPA_RS13045 (ACFMPA_13045) nucA/comI 2542714..2543124 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ACFMPA_RS13050 (ACFMPA_13050) - 2543321..2543671 (+) 351 Protein_2521 sigma-70 family RNA polymerase sigma factor -
  ACFMPA_RS13055 (ACFMPA_13055) spoIVCA 2543699..2545159 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  ACFMPA_RS13060 (ACFMPA_13060) - 2545117..2545295 (-) 179 Protein_2523 hypothetical protein -
  ACFMPA_RS13065 (ACFMPA_13065) - 2545650..2546069 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  ACFMPA_RS13070 (ACFMPA_13070) acr3 2546081..2547121 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  ACFMPA_RS13075 (ACFMPA_13075) - 2547144..2547584 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  ACFMPA_RS13080 (ACFMPA_13080) arsR 2547645..2547962 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1068237 ACFMPA_RS13045 WP_009967785.1 2542714..2543124(-) (nucA/comI) [Bacillus sp. SG20032]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1068237 ACFMPA_RS13045 WP_009967785.1 2542714..2543124(-) (nucA/comI) [Bacillus sp. SG20032]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCAGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment