Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACG1BZ_RS22820 Genome accession   NZ_CP172449
Coordinates   5157638..5158588 (-) Length   316 a.a.
NCBI ID   WP_282934620.1    Uniprot ID   -
Organism   Microbulbifer sp. CNSA002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5152638..5163588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG1BZ_RS22795 (ACG1BZ_22795) trmB 5153485..5154270 (-) 786 WP_413663810.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACG1BZ_RS22800 (ACG1BZ_22800) - 5154355..5154732 (-) 378 WP_413663811.1 DUF423 domain-containing protein -
  ACG1BZ_RS22805 (ACG1BZ_22805) rpoH 5154841..5155695 (-) 855 WP_282930650.1 RNA polymerase sigma factor RpoH -
  ACG1BZ_RS22810 (ACG1BZ_22810) ftsX 5155918..5156934 (-) 1017 WP_413663812.1 permease-like cell division protein FtsX -
  ACG1BZ_RS22815 (ACG1BZ_22815) ftsE 5156915..5157601 (-) 687 WP_282930649.1 cell division ATP-binding protein FtsE -
  ACG1BZ_RS22820 (ACG1BZ_22820) pilA 5157638..5158588 (-) 951 WP_282934620.1 signal recognition particle-docking protein FtsY Machinery gene
  ACG1BZ_RS22825 (ACG1BZ_22825) rsmD 5159097..5159687 (+) 591 WP_413663813.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACG1BZ_RS22830 (ACG1BZ_22830) coaD 5159774..5160250 (+) 477 WP_282930647.1 pantetheine-phosphate adenylyltransferase -
  ACG1BZ_RS22835 (ACG1BZ_22835) ggt 5160313..5162037 (+) 1725 WP_413663814.1 gamma-glutamyltransferase -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 34118.02 Da        Isoelectric Point: 5.7614

>NTDB_id=1067903 ACG1BZ_RS22820 WP_282934620.1 5157638..5158588(-) (pilA) [Microbulbifer sp. CNSA002]
MKPEAKKKEGFFARIRRGLSRTSNQFVEGMGNLFLGAKEIDEDLLEELETQLLMADVGVEATSEIVERLTERVSRRELAD
GDALYKALQQELADLLDSVDAPLEIDISKKPYVILVVGVNGVGKTTTIGKLAHRFLGEGKSVMLAAGDTFRAAAVEQLQV
WGQRHDVPVVAQHTGADSASVIFDAVQSAQARGVDVVIADTAGRLHTKSNLMEELSKVRRVMGKLDPSAPHEVLLVLDAG
TGQNAINQASQFREAAGVTGLVLTKLDGTAKGGVIFALARRFGIPVRFIGVGEQAEDLQPFRAREFVSALFGRDKA

Nucleotide


Download         Length: 951 bp        

>NTDB_id=1067903 ACG1BZ_RS22820 WP_282934620.1 5157638..5158588(-) (pilA) [Microbulbifer sp. CNSA002]
CTGAAACCGGAAGCCAAAAAGAAAGAGGGCTTCTTTGCGCGTATTCGCCGCGGCCTCAGCCGCACCAGCAACCAGTTTGT
CGAGGGGATGGGAAACCTGTTCCTCGGGGCCAAGGAGATCGATGAAGATCTGCTGGAAGAACTTGAAACACAGCTGTTAA
TGGCAGATGTGGGAGTTGAGGCCACCAGCGAGATCGTCGAGCGCCTGACCGAACGGGTGTCCCGTCGGGAACTGGCCGAT
GGCGATGCACTTTACAAGGCATTGCAGCAGGAGCTGGCTGACTTACTCGATAGTGTTGATGCTCCCCTGGAAATCGATAT
CAGCAAAAAGCCTTACGTCATCCTGGTAGTGGGCGTAAACGGTGTGGGTAAAACCACAACAATCGGCAAGCTGGCTCACC
GCTTTCTCGGTGAAGGCAAGTCGGTGATGTTGGCTGCCGGTGATACATTCCGTGCCGCAGCCGTTGAGCAGCTACAGGTT
TGGGGGCAGCGCCACGATGTACCGGTTGTAGCGCAGCATACCGGTGCCGATAGTGCGTCAGTTATTTTCGATGCGGTTCA
GTCTGCCCAGGCGCGCGGCGTCGATGTGGTAATTGCCGATACCGCCGGGCGCCTGCACACCAAATCCAACTTGATGGAGG
AGTTGTCCAAGGTGCGCCGGGTGATGGGCAAACTGGACCCCAGCGCGCCCCACGAGGTGCTATTGGTTCTGGATGCTGGC
ACTGGCCAAAATGCGATTAACCAGGCCTCTCAGTTCCGTGAGGCGGCCGGCGTTACTGGCTTGGTGCTGACCAAATTGGA
TGGTACTGCCAAAGGCGGCGTTATCTTTGCCCTGGCTCGCCGTTTCGGCATCCCGGTGCGTTTTATCGGTGTGGGTGAGC
AGGCGGAAGATCTACAGCCATTCCGCGCTCGTGAGTTCGTATCCGCCCTGTTTGGCCGCGATAAGGCCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(112-311)

SRP54-type protein, helical bundle domain (SRP54_N)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

54.276

96.203

0.522

Multiple sequence alignment