Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACAM55_RS22205 Genome accession   NZ_AP028664
Coordinates   4695351..4696946 (-) Length   531 a.a.
NCBI ID   WP_369653593.1    Uniprot ID   -
Organism   Variovorax sp. V213     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4690351..4701946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM55_RS22180 (GmRootV213_43880) - 4691668..4692267 (+) 600 WP_369653588.1 cytochrome b -
  ACAM55_RS22185 (GmRootV213_43890) - 4692279..4692857 (+) 579 WP_369653589.1 YceI family protein -
  ACAM55_RS22190 (GmRootV213_43900) - 4692916..4693548 (+) 633 WP_369653590.1 YceI family protein -
  ACAM55_RS22195 (GmRootV213_43910) - 4693609..4693734 (+) 126 WP_369653591.1 hypothetical protein -
  ACAM55_RS22200 (GmRootV213_43920) - 4693736..4695340 (-) 1605 WP_369653592.1 phospholipase D family protein -
  ACAM55_RS22205 (GmRootV213_43930) rpoN/rpoN1 4695351..4696946 (-) 1596 WP_369653593.1 RNA polymerase factor sigma-54 Machinery gene
  ACAM55_RS22210 (GmRootV213_43940) lptB 4696964..4697758 (-) 795 WP_369653594.1 LPS export ABC transporter ATP-binding protein -
  ACAM55_RS22215 (GmRootV213_43950) lptA 4697755..4698483 (-) 729 WP_369653595.1 lipopolysaccharide transport periplasmic protein LptA -
  ACAM55_RS22220 (GmRootV213_43960) - 4698638..4699285 (-) 648 WP_369653596.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACAM55_RS22225 (GmRootV213_43970) - 4699458..4700195 (-) 738 WP_369653597.1 SPOR domain-containing protein -
  ACAM55_RS22230 (GmRootV213_43980) argS 4700203..4701888 (-) 1686 WP_369653598.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 531 a.a.        Molecular weight: 58572.86 Da        Isoelectric Point: 4.7200

>NTDB_id=106778 ACAM55_RS22205 WP_369653593.1 4695351..4696946(-) (rpoN/rpoN1) [Variovorax sp. V213]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAIDTPVPRDESSGEAGES
DFASAPTSSAATTSSETTAAADTDIPAADATEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQES
LQSFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPAGVGARD
LGECLGIQLRALASEDETEEHALVRKTAIAICKQPMELLARRDFKRLATLTRTNEDLVRSALQIIARLEPKPGRRFVDVE
RNIVIPDVIVTKIGRGTNVKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSN
AIVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALIK
QFVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1596 bp        

>NTDB_id=106778 ACAM55_RS22205 WP_369653593.1 4695351..4696946(-) (rpoN/rpoN1) [Variovorax sp. V213]
ATGAAGCAAGGGCTGTCCCTTCGCGTCTCGCAGCATCTGGCGCTCACGCCCCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCCACGCTCGAACTGAGCCAGGAGGTCGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CGGCGCGCGAGGAATTCGGCCTCGATGCCATCGATACCCCGGTGCCGCGCGATGAGTCGTCGGGCGAGGCCGGCGAGAGC
GACTTCGCCTCGGCCCCGACGAGCAGCGCGGCCACCACATCCAGCGAAACCACCGCGGCGGCCGACACCGACATTCCCGC
CGCCGACGCCACCGAGCGCGAGCCCGACTGGGAGGGCGACGGCACCGTCGACATGGCGCCGGACGACAGCGAGTGGGGCA
GCGACGCCCCCGCGCGCCAGAACAATCTTGGCGACGACGAGCGCGCCGACGCCACCGAACTCGCGCGCAGCCAGGAGTCG
CTGCAGTCCTTCCTGCACCGGCAGGCGCTCAGCCTCCGGCTCAACGAGAACGACAGCGCCGCGCTGCGCTTCCTGATCGA
ATCGCTCAACGACGACGGCTACCTCGAAGATTCGCTGCCGGCGCTGGCCTCGGGCCTCGCGGGCGACGACAACGACCAGT
TCGACGAACTGGTGCATCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCGGCCGGTGTCGGCGCGCGCGAC
CTGGGCGAATGCCTCGGCATCCAGCTGCGCGCGCTCGCGAGCGAAGACGAAACCGAAGAGCATGCCCTGGTCCGCAAGAC
CGCCATTGCCATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACCCGCACCA
ACGAAGACCTGGTGCGCTCGGCGCTGCAGATCATCGCGCGCCTGGAGCCCAAGCCGGGCCGCCGTTTTGTCGACGTCGAG
CGCAACATCGTGATCCCCGACGTGATCGTCACCAAGATCGGCCGCGGCACCAACGTCAAGTTCCGCGTCATGCTCAACCC
CGAGGTGATGCCGCGGCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGC
TGTCGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTCAGCAAC
GCCATCGTCGAGCGGCAGAAAAGCTACTTCGTGCACGGCGAGCTCGCGATGCGGCCGCTGGTGCTGCGCGAGATCGCCGA
CGAGCTCGGCCTGCACGAATCGACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACGGTCGAGC
TCAAGTACTTCTTCGGCTCAGCGCTCGGCACCGAGACCGGCGGCAATGCATCGAGCACCGCGGTGCGCGCGCTGATCAAG
CAGTTCGTGAGTTCCGAGAGCATCAAGAAACCGCTGTCCGACAGCCAGATTTCCGAGATGCTCAAGGAGCAGGGCATCGA
ATGCGCGCGCCGCACCGTGGCCAAGTACCGCGAGGCGCTGCGCATCGCGCCCGCCAACCTGCGCAAGGCGTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(152-359)

Sigma-54, DNA binding domain (Sigma54_DBD)

(373-529)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.963

100

0.524

Multiple sequence alignment