Detailed information NTG1067628

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: ACHGMI_RS22735
• Genome accession: NZ_CP172417
• Coordinates: 4161713..4162858 (-)
• Length: 381 a.a.
• NCBI ID: WP_017696253.1
• Uniprot ID: A0A0C3JF17
• Organism: Bacillus subtilis strain AKPS2
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 4156713..4167858

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACHGMI_RS22710	pbpG	4156973..4159048 (-)	2076	WP_017696252.1	transglycosylase domain-containing protein	-
ACHGMI_RS22715	speE	4159251..4160081 (+)	831	WP_003227543.1	spermidine synthase	-
ACHGMI_RS22720	speB	4160142..4161014 (+)	873	WP_003227545.1	agmatinase	-
ACHGMI_RS22725	-	4161046..4161511 (-)	466	Protein_4424	YbaK/EbsC family protein	-
ACHGMI_RS22730	-	4161610..4161729 (-)	120	WP_014665774.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
ACHGMI_RS22735	rapF	4161713..4162858 (-)	1146	WP_017696253.1	tetratricopeptide repeat protein	Regulator
ACHGMI_RS22740	-	4162861..4163091 (+)	231	WP_017696254.1	hypothetical protein	-
ACHGMI_RS22745	ywhK	4163088..4164443 (+)	1356	WP_017696255.1	YncE family protein	-
ACHGMI_RS22750	ywhL	4164492..4165859 (+)	1368	WP_017696256.1	YncE family protein	-
ACHGMI_RS22755	albG	4165865..4166566 (-)	702	WP_017696257.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
ACHGMI_RS22760	albF	4166563..4167843 (-)	1281	WP_017696258.1	M16 family metallopeptidase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45593.24 Da
• Isoelectric Point: 5.0684

>NTDB_id=1067628 ACHGMI_RS22735 WP_017696253.1 4161713..4162858(-) (rapF) [Bacillus subtilis strain AKPS2]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDRREYVLAIQFFKKAESKLVFVKDKIDKAEFFFKMSEVYYYMKQTYVS
MDYARQAFEIYSEQTTYDIRMIQCQSLFGANFLDLKQYEKAIAHFQKAFTLAKTEQQPQLMGRTLYNIALCKQNQNSFES
AIEYVKKSIQVFEGSKMLPSLPQAFFLITQIYFKLGKIDNACEYHKKGKEYSAAAGDTVYLTEFEFLESLYLSGPDEERI
EKCFVFLESKMMYADIEDFALDVAKYYHQQENYKKASHYFLKVEEARQHIQGGVNLYEMEV

Nucleotide

• Length: 1146 bp

>NTDB_id=1067628 ACHGMI_RS22735 WP_017696253.1 4161713..4162858(-) (rapF) [Bacillus subtilis strain AKPS2]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTTAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCGCCGTGAATATGTATTAGCCATTCAATTCTTTAAAAAAGCTGAGAGCAAGCTGGTTTTTG
TCAAAGATAAAATCGATAAAGCTGAATTCTTTTTCAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTCTCC
ATGGACTATGCACGTCAAGCATTCGAAATATACAGTGAACAGACTACATATGATATCAGAATGATTCAATGCCAGTCCTT
ATTCGGAGCCAACTTTTTGGATTTAAAACAATACGAAAAAGCAATTGCCCATTTTCAAAAAGCCTTTACACTTGCAAAAA
CAGAGCAGCAGCCCCAATTAATGGGAAGAACACTTTATAACATAGCACTCTGCAAGCAAAACCAAAACAGTTTTGAATCA
GCGATAGAGTATGTAAAAAAATCTATTCAAGTATTTGAAGGATCTAAAATGCTTCCTTCATTGCCACAAGCTTTTTTCCT
AATTACACAAATTTATTTTAAATTAGGTAAGATTGATAACGCCTGTGAATATCATAAAAAAGGAAAAGAGTATTCTGCAG
CTGCGGGAGATACCGTGTATCTAACGGAGTTTGAATTTTTGGAATCTTTATACTTGTCTGGACCGGATGAGGAACGGATA
GAAAAATGTTTTGTTTTTCTTGAAAGTAAAATGATGTATGCAGATATTGAGGACTTCGCACTTGATGTTGCTAAATATTA
TCATCAACAGGAAAATTACAAAAAAGCATCTCATTACTTTTTAAAGGTTGAAGAAGCAAGACAGCACATTCAAGGGGGGG
TGAATTTATATGAAATGGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_8) (263-290)

• Tetratricopeptide repeat (TPR_12) (189-253)

3D structure

• AlphaFold DB: A0A0C3JF17

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	80.052	100	0.801
rapC	Bacillus subtilis subsp. subtilis str. 168	57.105	99.738	0.57