Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACHGMI_RS14575 Genome accession   NZ_CP172417
Coordinates   2665146..2665913 (+) Length   255 a.a.
NCBI ID   WP_017696274.1    Uniprot ID   -
Organism   Bacillus subtilis strain AKPS2     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2660146..2670913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHGMI_RS14545 pgpH 2660442..2662577 (+) 2136 WP_080031083.1 cyclic-di-AMP phosphodiesterase PgpH -
  ACHGMI_RS14550 ybeY 2662578..2663051 (+) 474 WP_014480295.1 rRNA maturation RNase YbeY -
  ACHGMI_RS14555 unpK 2663032..2663403 (+) 372 WP_015384119.1 diacylglycerol kinase family protein -
  ACHGMI_RS14560 - 2663522..2663932 (+) 411 WP_014480293.1 cytidine deaminase -
  ACHGMI_RS14565 era 2663913..2664818 (+) 906 WP_003230051.1 GTPase Era -
  ACHGMI_RS14570 yqzL 2664966..2665109 (+) 144 WP_003230053.1 YqzL family protein -
  ACHGMI_RS14575 recO 2665146..2665913 (+) 768 WP_017696274.1 DNA repair protein RecO Machinery gene
  ACHGMI_RS14580 glyQ 2666210..2667097 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ACHGMI_RS14585 glyS 2667090..2669129 (+) 2040 WP_014480291.1 glycine--tRNA ligase subunit beta -
  ACHGMI_RS14590 ccpN 2669262..2669900 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  ACHGMI_RS14595 ppsR 2669926..2670738 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29346.03 Da        Isoelectric Point: 8.3080

>NTDB_id=1067597 ACHGMI_RS14575 WP_017696274.1 2665146..2665913(+) (recO) [Bacillus subtilis strain AKPS2]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKTNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1067597 ACHGMI_RS14575 WP_017696274.1 2665146..2665913(+) (recO) [Bacillus subtilis strain AKPS2]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGAC
AAATCCTTATTTATTTGAATTCATATTAGAGTCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment