Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACAM52_RS25815 Genome accession   NZ_AP028663
Coordinates   5562865..5564454 (-) Length   529 a.a.
NCBI ID   WP_062480326.1    Uniprot ID   -
Organism   Variovorax sp. V118     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5557865..5569454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM52_RS25785 (GmRootV118_51170) - 5558017..5558628 (+) 612 WP_369644942.1 cytochrome b -
  ACAM52_RS25790 (GmRootV118_51180) - 5558625..5559209 (+) 585 WP_095743358.1 YceI family protein -
  ACAM52_RS25795 (GmRootV118_51190) - 5559248..5559880 (+) 633 WP_095743357.1 YceI family protein -
  ACAM52_RS25800 (GmRootV118_51200) - 5559906..5560550 (+) 645 WP_062480320.1 LysE family translocator -
  ACAM52_RS25805 (GmRootV118_51210) - 5560724..5561170 (+) 447 WP_369644943.1 hypothetical protein -
  ACAM52_RS25810 (GmRootV118_51220) - 5561238..5562854 (-) 1617 WP_062480324.1 phospholipase D family protein -
  ACAM52_RS25815 (GmRootV118_51230) rpoN/rpoN1 5562865..5564454 (-) 1590 WP_062480326.1 RNA polymerase factor sigma-54 Machinery gene
  ACAM52_RS25820 (GmRootV118_51240) lptB 5564472..5565266 (-) 795 WP_062480328.1 LPS export ABC transporter ATP-binding protein -
  ACAM52_RS25825 (GmRootV118_51250) lptA 5565263..5565982 (-) 720 WP_369644944.1 lipopolysaccharide transport periplasmic protein LptA -
  ACAM52_RS25830 (GmRootV118_51260) - 5566104..5566748 (-) 645 WP_062483056.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACAM52_RS25835 (GmRootV118_51270) - 5566905..5567669 (-) 765 WP_369644945.1 SPOR domain-containing protein -
  ACAM52_RS25840 (GmRootV118_51280) argS 5567684..5569369 (-) 1686 WP_369644946.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58340.59 Da        Isoelectric Point: 4.6825

>NTDB_id=106754 ACAM52_RS25815 WP_062480326.1 5562865..5564454(-) (rpoN/rpoN1) [Variovorax sp. V118]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDTADAPVPRDDAGEGEAEF
TPSAASTSSTSSDTSTSTPDTDGPAAEIAEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESLQ
SFLHRQALSLRLNENDCAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPVGVGARDLG
ECLSIQLKALASEDETEAEALVRKTAIAICKQPMELLARRDFKRLATLTRTNEDLVRSALQVIARLEPKPGRRFVDVERN
IVIPDVIVTKTGRGTNVKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=106754 ACAM52_RS25815 WP_062480326.1 5562865..5564454(-) (rpoN/rpoN1) [Variovorax sp. V118]
ATGAAGCAAGGGCTGTCCCTTCGCGTCTCGCAGCATCTGGCGCTGACGCCACAGCTGCAGCAGTCCATTCGCCTGCTCCA
ACTCTCCACGCTCGAACTGAGCCAGGAAGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CGGCGCGCGAGGAATTCGGGCTGGACACGGCCGATGCGCCGGTGCCGCGCGACGACGCGGGCGAGGGCGAGGCCGAGTTC
ACGCCGTCCGCCGCCAGCACTTCCTCCACGTCCAGCGACACCAGCACCTCGACCCCCGACACCGACGGCCCCGCCGCCGA
GATCGCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAGTGGGGCAGCGATG
CGCCCGCGCGCCAGAACAACCTGGGCGACGACGAGCGTGCCGACGCCACCGAACTCGCGCGCAGCCAGGAGTCGCTGCAG
TCCTTCCTGCACCGCCAGGCGCTGAGCCTGCGGCTCAATGAAAACGACTGCGCCGCGCTGCGCTTCCTGATCGAGTCGCT
CAACGACGACGGCTACCTCGAAGACTCGCTGCCCGCGCTGGCTTCGGGCCTGGCGGGCGACGACAACGACCAGTTCGACG
ACCTCGTGCACCACTTCCAGGTGGCGCTGGGCCTGCTGCAGAGCCTGGAGCCGGTGGGCGTCGGCGCGCGCGACCTCGGC
GAATGCCTGAGCATCCAGCTGAAGGCGCTCGCCAGCGAAGACGAGACCGAGGCCGAAGCGCTCGTGCGCAAGACCGCCAT
CGCGATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGCACCAACGAAG
ACCTGGTGCGCTCGGCGCTGCAGGTCATTGCGCGGCTCGAACCCAAGCCGGGGCGCCGCTTTGTCGACGTCGAGCGCAAC
ATCGTCATCCCCGACGTGATCGTCACCAAGACCGGGCGCGGCACCAACGTGAAGTTCCGCGTCATGCTCAACCCCGAGGT
CATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGTCGC
AGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCCATC
GTCGAGCGCCAGAAGAGCTACTTCGTGCACGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
GGGCCTGCACGAGTCGACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACGGTCGAGCTGAAGT
ACTTCTTCGGTTCGGCGCTGGGCACCGAGACCGGCGGCAACGCGTCGAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTC
GTGAGTTCCGAGAGCATCAAGAAGCCGCTGTCCGACAGCCAGATTTCCGAGATGCTGAAAGAGCAGGGCATCGAGTGCGC
GCGCCGCACCGTCGCCAAGTACCGCGAAGCGCTGCGCATCGCGCCCGCCAATCTGCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.601

100

0.518

Multiple sequence alignment