Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   ACH6E8_RS06180 Genome accession   NZ_CP172380
Coordinates   1154802..1155761 (+) Length   319 a.a.
NCBI ID   WP_002875425.1    Uniprot ID   -
Organism   Campylobacter jejuni strain YH008     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1135097..1159804 1154802..1155761 within 0


Gene organization within MGE regions


Location: 1135097..1159804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH6E8_RS06085 (ACH6E8_06085) pglI 1135097..1136026 (-) 930 WP_002890470.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  ACH6E8_RS06090 (ACH6E8_06090) pglH 1136019..1137098 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  ACH6E8_RS06095 (ACH6E8_06095) pglK 1137095..1138789 (-) 1695 WP_002890469.1 ABC-type lipopolysaccharide transporter PglK -
  ACH6E8_RS06100 (ACH6E8_06100) galE 1138783..1139769 (-) 987 WP_002853529.1 UDP-glucose 4-epimerase GalE -
  ACH6E8_RS06105 (ACH6E8_06105) - 1139827..1140621 (-) 795 WP_002853812.1 3'-5' exonuclease -
  ACH6E8_RS06110 (ACH6E8_06110) waaC 1140687..1141715 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  ACH6E8_RS06115 (ACH6E8_06115) htrB 1141705..1142592 (+) 888 WP_002890465.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  ACH6E8_RS06120 (ACH6E8_06120) - 1142589..1144136 (+) 1548 WP_002858148.1 glycosyltransferase -
  ACH6E8_RS06125 (ACH6E8_06125) - 1144140..1145312 (+) 1173 WP_002858013.1 glycosyltransferase family 2 protein -
  ACH6E8_RS06130 (ACH6E8_06130) - 1145296..1146291 (-) 996 WP_002858358.1 capsular polysaccharide synthesis protein -
  ACH6E8_RS06135 (ACH6E8_06135) - 1146349..1147518 (+) 1170 WP_002858320.1 glycosyltransferase family 2 protein -
  ACH6E8_RS06140 (ACH6E8_06140) wlaN 1147507..1148420 (-) 914 Protein_1190 beta-1,3 galactosyltransferase -
  ACH6E8_RS06145 (ACH6E8_06145) cstIII 1148475..1149359 (+) 885 WP_002858290.1 alpha-2,3-sialyltransferase -
  ACH6E8_RS06150 (ACH6E8_06150) neuB1 1149340..1150371 (+) 1032 WP_002858213.1 sialic acid synthase -
  ACH6E8_RS06155 (ACH6E8_06155) neuC 1150368..1151482 (+) 1115 Protein_1193 UDP-N-acetylglucosamine 2-epimerase -
  ACH6E8_RS06160 (ACH6E8_06160) - 1151479..1152417 (+) 939 Protein_1194 beta-1,4-N-acetylgalactosaminyltransferase -
  ACH6E8_RS06165 (ACH6E8_06165) - 1152427..1153089 (+) 663 WP_002881429.1 cytidylyltransferase domain-containing protein -
  ACH6E8_RS06170 (ACH6E8_06170) - 1153084..1153935 (-) 852 WP_014516804.1 glycosyltransferase -
  ACH6E8_RS06175 (ACH6E8_06175) waaV 1153919..1154743 (-) 825 WP_002858096.1 glycosyltransferase family 2 protein -
  ACH6E8_RS06180 (ACH6E8_06180) waaF 1154802..1155761 (+) 960 WP_002875425.1 lipopolysaccharide heptosyltransferase II Regulator
  ACH6E8_RS06185 (ACH6E8_06185) gmhA 1155742..1156302 (-) 561 WP_002858021.1 D-sedoheptulose 7-phosphate isomerase -
  ACH6E8_RS06190 (ACH6E8_06190) rfaE1 1156299..1157684 (-) 1386 WP_002890180.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  ACH6E8_RS06195 (ACH6E8_06195) rfaD 1157677..1158630 (-) 954 WP_002852832.1 ADP-glyceromanno-heptose 6-epimerase -
  ACH6E8_RS06200 (ACH6E8_06200) gmhB 1158631..1159191 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  ACH6E8_RS06205 (ACH6E8_06205) - 1159273..1159575 (+) 303 WP_002852762.1 cytochrome c -
  ACH6E8_RS06210 (ACH6E8_06210) ccoS 1159598..1159804 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36734.30 Da        Isoelectric Point: 10.2412

>NTDB_id=1067028 ACH6E8_RS06180 WP_002875425.1 1154802..1155761(+) (waaF) [Campylobacter jejuni strain YH008]
MKIFIHLPTWLGDTVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLILRNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHDILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEKVIEQAKNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=1067028 ACH6E8_RS06180 WP_002875425.1 1154802..1155761(+) (waaF) [Campylobacter jejuni strain YH008]
ATGAAAATTTTTATACATCTTCCCACCTGGTTAGGCGATACGGTAATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCTACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTCTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAATTCACTTAGTATAAAAGCTCATTTTAAGGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAGAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGATATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAAAAGTGATAGAACAGGCTAAAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

100

100

1

Multiple sequence alignment