Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACAM53_RS02575 Genome accession   NZ_AP028657
Coordinates   574300..575892 (+) Length   530 a.a.
NCBI ID   WP_145742540.1    Uniprot ID   -
Organism   Variovorax sp. V11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 569300..580892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM53_RS02550 (GmRootV11_05090) argS 569330..571015 (+) 1686 WP_192323498.1 arginine--tRNA ligase -
  ACAM53_RS02555 (GmRootV11_05100) - 571024..571764 (+) 741 WP_145742548.1 SPOR domain-containing protein -
  ACAM53_RS02560 (GmRootV11_05110) - 571909..572556 (+) 648 WP_145742546.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACAM53_RS02565 (GmRootV11_05120) lptA 572754..573491 (+) 738 WP_369649737.1 lipopolysaccharide transport periplasmic protein LptA -
  ACAM53_RS02570 (GmRootV11_05130) lptB 573488..574282 (+) 795 WP_145742542.1 LPS export ABC transporter ATP-binding protein -
  ACAM53_RS02575 (GmRootV11_05140) rpoN/rpoN1 574300..575892 (+) 1593 WP_145742540.1 RNA polymerase factor sigma-54 Machinery gene
  ACAM53_RS02580 (GmRootV11_05150) - 575903..577510 (+) 1608 WP_369649738.1 phospholipase D family protein -
  ACAM53_RS02585 (GmRootV11_05160) - 577521..578162 (-) 642 WP_369649739.1 LysE family translocator -
  ACAM53_RS02590 (GmRootV11_05170) - 578169..578804 (-) 636 WP_145742535.1 YceI family protein -
  ACAM53_RS02595 (GmRootV11_05180) - 578846..579424 (-) 579 WP_369649740.1 YceI family protein -
  ACAM53_RS02600 (GmRootV11_05190) - 579421..580047 (-) 627 WP_209503117.1 cytochrome b -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58420.77 Da        Isoelectric Point: 4.7226

>NTDB_id=106696 ACAM53_RS02575 WP_145742540.1 574300..575892(+) (rpoN/rpoN1) [Variovorax sp. V11]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAIDTPVPRDEAGEAAEGE
FASAPASSATTTSSETSSAAEADVPAADATEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESL
QSFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPAGVGARDL
GECLCIQLRALASESETEEQALVRKTAIAICKQPMELLARRDFKRLATLTRTNEEVVRSALQIISRLEPKPGRRFVDVER
NIVIPDVIVTKIGRGTNAKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNA
IVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALIKQ
FVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=106696 ACAM53_RS02575 WP_145742540.1 574300..575892(+) (rpoN/rpoN1) [Variovorax sp. V11]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCACCTGGCGCTCACGCCCCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAACTGAGCCAGGAGGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CGGCGCGCGAGGAGTTCGGGCTCGACGCCATCGACACGCCGGTGCCGCGCGACGAAGCCGGTGAAGCCGCCGAGGGCGAA
TTCGCCTCGGCCCCGGCCAGCAGCGCAACCACCACTTCGAGCGAAACCTCCTCGGCAGCCGAGGCCGACGTACCCGCCGC
CGACGCCACCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAATGGGGCAGCG
ACGCCCCCGCGCGCCAGAACAACCTGGGCGACGACGAACGCGCCGACGCCACCGAGCTCGCGCGCAGCCAGGAGTCGCTG
CAGTCCTTCCTGCACCGGCAGGCGCTGAGCCTGCGGCTCAACGAGAACGACAGCGCCGCGCTGCGCTTCCTGATCGAATC
GCTCAACGACGACGGCTACCTCGAAGACTCCCTGCCGGCGCTGGCCTCGGGCCTCGCGGGCGACGACAACGACCAGTTCG
ACGAACTGGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAAAGCCTGGAGCCGGCCGGCGTCGGCGCGCGCGATCTG
GGCGAATGCCTGTGCATCCAGCTGCGCGCGCTGGCCAGCGAAAGCGAGACCGAAGAGCAGGCCCTGGTGCGCAAGACCGC
CATCGCGATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGATTTCAAGCGCCTGGCCACGCTCACGCGCACCAACG
AAGAGGTGGTGCGCTCGGCGCTGCAGATCATCTCGCGCCTGGAGCCCAAGCCGGGCCGCCGCTTCGTCGACGTCGAGCGC
AACATCGTGATTCCCGACGTGATCGTCACCAAGATCGGCCGCGGCACCAACGCCAAGTTCCGCGTCATGCTCAACCCCGA
GGTGATGCCGCGGCTGCGCGTGCACGACATCTATGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGT
CGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCC
ATCGTCGAGCGGCAGAAGAGCTTCTTCGTGCACGGCGAGCTCGCAATGCGGCCCTTGGTGCTGCGCGAGATCGCCGACGA
ACTCGGCCTGCACGAATCGACCATCTCGCGCGTGACCACGGCCAAGTACATGGCCACGCCTTTCGGCACGGTCGAGCTCA
AGTACTTCTTCGGCTCGGCGCTCGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCCGTGCGCGCGCTGATCAAGCAG
TTCGTGAGCTCCGAGAGCATCAAGAAACCGCTGTCCGACAGCCAGATCTCCGAGATGCTCAAGGAGCAGGGCATCGAGTG
CGCACGCCGCACCGTGGCCAAGTACCGCGAGGCCTTGCGCATCGCGCCCGCCAACCTTCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-358)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(372-528)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.166

100

0.523

Multiple sequence alignment