Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   ACH6FD_RS06000 Genome accession   NZ_CP172362
Coordinates   1154225..1155184 (+) Length   319 a.a.
NCBI ID   WP_002876195.1    Uniprot ID   -
Organism   Campylobacter jejuni strain YH022     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1134525..1159227 1154225..1155184 within 0


Gene organization within MGE regions


Location: 1134525..1159227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH6FD_RS05905 (ACH6FD_05905) pglI 1134525..1135454 (-) 930 WP_002867212.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  ACH6FD_RS05910 (ACH6FD_05910) pglH 1135447..1136526 (-) 1080 WP_079264556.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  ACH6FD_RS05915 (ACH6FD_05915) pglK 1136523..1138217 (-) 1695 WP_002869587.1 ABC-type lipopolysaccharide transporter PglK -
  ACH6FD_RS05920 (ACH6FD_05920) galE 1138211..1139197 (-) 987 WP_002869588.1 UDP-glucose 4-epimerase GalE -
  ACH6FD_RS05925 (ACH6FD_05925) - 1139256..1140050 (-) 795 WP_002860221.1 3'-5' exonuclease -
  ACH6FD_RS05930 (ACH6FD_05930) waaC 1140116..1141144 (+) 1029 WP_002858077.1 lipopolysaccharide heptosyltransferase I -
  ACH6FD_RS05935 (ACH6FD_05935) htrB 1141134..1142021 (+) 888 WP_002858051.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  ACH6FD_RS05940 (ACH6FD_05940) - 1142018..1143565 (+) 1548 WP_002858148.1 glycosyltransferase -
  ACH6FD_RS05945 (ACH6FD_05945) - 1143569..1144741 (+) 1173 WP_002858013.1 glycosyltransferase family 2 protein -
  ACH6FD_RS05950 (ACH6FD_05950) - 1144725..1145720 (-) 996 WP_002858358.1 capsular polysaccharide synthesis protein -
  ACH6FD_RS05955 (ACH6FD_05955) - 1145778..1146947 (+) 1170 WP_002858320.1 glycosyltransferase family 2 protein -
  ACH6FD_RS05960 (ACH6FD_05960) wlaN 1146936..1147846 (-) 911 Protein_1154 beta-1,3 galactosyltransferase -
  ACH6FD_RS05965 (ACH6FD_05965) cstIII 1147901..1148785 (+) 885 WP_002858290.1 alpha-2,3-sialyltransferase -
  ACH6FD_RS05970 (ACH6FD_05970) neuB1 1148766..1149797 (+) 1032 WP_002858213.1 sialic acid synthase -
  ACH6FD_RS05975 (ACH6FD_05975) neuC 1149794..1150909 (+) 1116 WP_002858400.1 UDP-N-acetylglucosamine 2-epimerase -
  ACH6FD_RS05980 (ACH6FD_05980) - 1150906..1151844 (+) 939 Protein_1158 beta-1,4-N-acetylgalactosaminyltransferase -
  ACH6FD_RS05985 (ACH6FD_05985) - 1151854..1152516 (+) 663 WP_002881429.1 cytidylyltransferase domain-containing protein -
  ACH6FD_RS05990 (ACH6FD_05990) - 1152511..1153358 (-) 848 Protein_1160 glycosyltransferase -
  ACH6FD_RS05995 (ACH6FD_05995) waaV 1153342..1154166 (-) 825 WP_052796721.1 glycosyltransferase family 2 protein -
  ACH6FD_RS06000 (ACH6FD_06000) waaF 1154225..1155184 (+) 960 WP_002876195.1 lipopolysaccharide heptosyltransferase II Regulator
  ACH6FD_RS06005 (ACH6FD_06005) gmhA 1155165..1155725 (-) 561 WP_002876196.1 D-sedoheptulose 7-phosphate isomerase -
  ACH6FD_RS06010 (ACH6FD_06010) rfaE1 1155722..1157107 (-) 1386 WP_002910336.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  ACH6FD_RS06015 (ACH6FD_06015) rfaD 1157100..1158053 (-) 954 WP_002886910.1 ADP-glyceromanno-heptose 6-epimerase -
  ACH6FD_RS06020 (ACH6FD_06020) gmhB 1158054..1158615 (-) 562 Protein_1166 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  ACH6FD_RS06025 (ACH6FD_06025) - 1158696..1158998 (+) 303 WP_002852762.1 cytochrome c -
  ACH6FD_RS06030 (ACH6FD_06030) ccoS 1159021..1159227 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36607.08 Da        Isoelectric Point: 10.2870

>NTDB_id=1066810 ACH6FD_RS06000 WP_002876195.1 1154225..1155184(+) (waaF) [Campylobacter jejuni strain YH022]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHSKDLKLPFKLKLQDPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKSEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENAILVHLDLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNSHR

Nucleotide


Download         Length: 960 bp        

>NTDB_id=1066810 ACH6FD_RS06000 WP_002876195.1 1154225..1155184(+) (waaF) [Campylobacter jejuni strain YH022]
ATGAAAATTTTTATACATCTTCCCACTTGGCTGGGCGATGCAGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGCTCTTTAGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTCTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAACTTCAAGACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAATCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAATCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAACAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAACGCAATATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAAACTCTCATCGATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

94.025

99.687

0.937

Multiple sequence alignment