Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACH19Q_RS14760 Genome accession   NZ_CP172236
Coordinates   3106934..3108433 (+) Length   499 a.a.
NCBI ID   WP_020831172.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain UMN24-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3101934..3113433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH19Q_RS14735 (ACH19Q_14735) - 3103127..3104110 (+) 984 WP_016724265.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACH19Q_RS14740 (ACH19Q_14740) - 3104149..3104736 (+) 588 WP_011000373.1 KdsC family phosphatase -
  ACH19Q_RS14745 (ACH19Q_14745) lptC 3104738..3105370 (+) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  ACH19Q_RS14750 (ACH19Q_14750) lptA 3105439..3106014 (+) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  ACH19Q_RS14755 (ACH19Q_14755) lptB 3106048..3106836 (+) 789 WP_197368341.1 LPS export ABC transporter ATP-binding protein -
  ACH19Q_RS14760 (ACH19Q_14760) rpoN/rpoN1 3106934..3108433 (+) 1500 WP_020831172.1 RNA polymerase factor sigma-54 Machinery gene
  ACH19Q_RS14765 (ACH19Q_14765) hpf 3108485..3108838 (+) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACH19Q_RS14770 (ACH19Q_14770) ptsN 3109187..3109642 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACH19Q_RS14775 (ACH19Q_14775) hprK 3109713..3110687 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  ACH19Q_RS14780 (ACH19Q_14780) - 3110878..3111213 (+) 336 WP_020425146.1 PsiF family protein -
  ACH19Q_RS14785 (ACH19Q_14785) rapZ 3111307..3112197 (+) 891 WP_011000365.1 RNase adapter RapZ -
  ACH19Q_RS14790 (ACH19Q_14790) - 3112275..3112925 (+) 651 WP_016724261.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55213.53 Da        Isoelectric Point: 4.8037

>NTDB_id=1065831 ACH19Q_RS14760 WP_020831172.1 3106934..3108433(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain UMN24-1]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTADASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1065831 ACH19Q_RS14760 WP_020831172.1 3106934..3108433(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain UMN24-1]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGTGCGGACCATGCCGACACAGCCGACGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

99.8

100

0.998

Multiple sequence alignment