Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACHMXE_RS16750 Genome accession   NZ_CP172004
Coordinates   948595..949539 (-) Length   314 a.a.
NCBI ID   WP_395350272.1    Uniprot ID   -
Organism   Variovorax sp. UC122_21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 943595..954539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXE_RS16730 (ACHMXE_16745) rpoH 944292..945234 (-) 943 Protein_900 RNA polymerase sigma factor RpoH -
  ACHMXE_RS16735 (ACHMXE_16750) - 945406..945817 (-) 412 Protein_901 DUF962 domain-containing protein -
  ACHMXE_RS16740 (ACHMXE_16755) - 946077..946781 (+) 705 WP_395352701.1 response regulator -
  ACHMXE_RS16745 (ACHMXE_16760) - 946792..948582 (+) 1791 WP_395350271.1 ATP-binding protein -
  ACHMXE_RS16750 (ACHMXE_16765) pilA 948595..949539 (-) 945 WP_395350272.1 signal recognition particle-docking protein FtsY Machinery gene
  ACHMXE_RS16755 (ACHMXE_16770) - 949939..951390 (+) 1452 WP_395350273.1 M16 family metallopeptidase -
  ACHMXE_RS16760 (ACHMXE_16775) - 951423..952811 (+) 1389 WP_395350274.1 M16 family metallopeptidase -
  ACHMXE_RS16765 (ACHMXE_16780) - 952818..953720 (-) 903 WP_395350276.1 LysR family transcriptional regulator ArgP -
  ACHMXE_RS16770 (ACHMXE_16785) - 953796..954431 (+) 636 WP_395350278.1 LysE/ArgO family amino acid transporter -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 33510.60 Da        Isoelectric Point: 5.7967

>NTDB_id=1064771 ACHMXE_RS16750 WP_395350272.1 948595..949539(-) (pilA) [Variovorax sp. UC122_21]
MPAPVLEIAPPPAAERRGWLDKLKTGLRKTGTGIQAVFVNAQIDDALYEELESALLMADAGVKATEYLLDDLRRRVKSQM
ATDASQVKRLLAEAIADLLRPLEKPLEIGRHTPTVIMVAGVNGAGKTTSIGKLTKHLANEGASVLLAAADTFRAAAREQL
LVWADRNTVEIVSQEGGDPSAVSFDAVNAGKARGKDVVLVDTAGRLPTQLHLMDELRKIKRVVTKADATAPHEVLLVIDG
NTGQNALAQVKAFDETLGLTGLVVTKLDGTAKGGVLCAIARERPIPVYFIGVGEKLEDLETFDAREFAQALLGN

Nucleotide


Download         Length: 945 bp        

>NTDB_id=1064771 ACHMXE_RS16750 WP_395350272.1 948595..949539(-) (pilA) [Variovorax sp. UC122_21]
GTGCCGGCACCGGTGCTCGAGATCGCCCCCCCGCCCGCAGCCGAGCGCCGGGGCTGGCTCGACAAGCTCAAGACCGGCCT
GCGCAAGACCGGCACCGGCATCCAGGCGGTGTTCGTCAATGCGCAGATCGACGACGCGCTCTACGAAGAGCTCGAATCCG
CGCTGCTGATGGCCGATGCCGGCGTGAAGGCGACCGAATACCTGCTCGACGACCTGCGCCGCCGCGTCAAGAGCCAGATG
GCCACCGACGCGTCGCAGGTCAAGCGCCTGCTGGCCGAGGCCATCGCCGACCTGCTGCGCCCACTCGAGAAGCCGCTGGA
GATCGGCCGCCACACGCCCACCGTGATCATGGTCGCGGGCGTGAACGGCGCGGGCAAGACCACCTCCATCGGCAAGCTCA
CCAAGCACCTGGCCAACGAGGGCGCCTCGGTGCTGCTGGCCGCGGCCGACACCTTCCGTGCCGCGGCGCGCGAGCAGCTG
CTGGTCTGGGCCGACCGCAACACGGTGGAGATCGTGAGCCAGGAAGGCGGCGACCCTTCCGCCGTGAGCTTCGACGCGGT
CAACGCCGGCAAGGCGCGCGGCAAGGACGTGGTGCTGGTCGACACCGCGGGCCGGCTGCCGACGCAGCTGCACCTGATGG
ACGAGCTCAGGAAGATCAAGCGCGTGGTCACCAAGGCCGACGCCACCGCGCCGCACGAGGTGCTGCTGGTGATCGACGGC
AACACCGGCCAGAACGCGCTGGCGCAGGTCAAGGCCTTCGACGAGACGCTGGGCCTGACCGGGCTGGTCGTGACCAAGCT
CGACGGCACCGCCAAGGGCGGCGTGCTGTGCGCGATCGCGCGCGAGCGGCCGATCCCGGTCTACTTCATCGGCGTGGGCG
AGAAGCTCGAGGACCTCGAGACCTTCGACGCGCGCGAGTTCGCGCAGGCCTTGCTGGGCAACTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(113-313)

SRP54-type protein, helical bundle domain (SRP54_N)

(31-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

58.667

95.541

0.561

Multiple sequence alignment