Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHMXC_RS05900 Genome accession   NZ_CP171997
Coordinates   1271634..1273235 (-) Length   533 a.a.
NCBI ID   WP_108498276.1    Uniprot ID   A0A2R7NZI6
Organism   Acidovorax delafieldii strain UC81_30     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1266634..1278235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXC_RS05870 (ACHMXC_05870) - 1267687..1268505 (+) 819 WP_395456746.1 hypothetical protein -
  ACHMXC_RS05875 (ACHMXC_05875) - 1268520..1268948 (+) 429 WP_395456748.1 GNAT family N-acetyltransferase -
  ACHMXC_RS05880 (ACHMXC_05880) - 1269048..1269269 (-) 222 WP_395456750.1 hypothetical protein -
  ACHMXC_RS05885 (ACHMXC_05885) - 1269306..1269791 (-) 486 WP_395456752.1 putative toxin-antitoxin system toxin component, PIN family -
  ACHMXC_RS05890 (ACHMXC_05890) - 1269781..1271073 (-) 1293 WP_395456754.1 class I SAM-dependent RNA methyltransferase -
  ACHMXC_RS05895 (ACHMXC_05895) - 1271070..1271552 (-) 483 WP_395456756.1 acyl-CoA thioesterase -
  ACHMXC_RS05900 (ACHMXC_05900) rpoN/rpoN1 1271634..1273235 (-) 1602 WP_108498276.1 RNA polymerase factor sigma-54 Machinery gene
  ACHMXC_RS05905 (ACHMXC_05905) lptB 1273235..1274017 (-) 783 WP_056065226.1 LPS export ABC transporter ATP-binding protein -
  ACHMXC_RS05910 (ACHMXC_05910) lptA 1274014..1274673 (-) 660 WP_056065224.1 lipopolysaccharide transport periplasmic protein LptA -
  ACHMXC_RS05915 (ACHMXC_05915) - 1274782..1275438 (-) 657 WP_056747786.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACHMXC_RS05920 (ACHMXC_05920) - 1275551..1276207 (-) 657 WP_056747785.1 SPOR domain-containing protein -
  ACHMXC_RS05925 (ACHMXC_05925) argS 1276223..1277932 (-) 1710 WP_395456760.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 58651.77 Da        Isoelectric Point: 4.6271

>NTDB_id=1064750 ACHMXC_RS05900 WP_108498276.1 1271634..1273235(-) (rpoN/rpoN1) [Acidovorax delafieldii strain UC81_30]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNADEAPREEFGLEAADTPAQADDYSADDAMF
SGAVSASSTSAESQNEAEIPSGGADEPDWSGDGTVEMAPNDAEWGGDAPARTRNDAEGDEADATELARSHESLTAFLHRQ
ALSLRLSEVDTAALRFLIESLNDDGYLEEPLEELATSLAGPDDLEQIEELVHRFTVAQRLLQSLEPVGVGARNLAECLTL
QLTALAADEDSDADPETVQTALRICQQPLEMLARRDIRRLTQLCSEGGVAGEERTRSAMALIARLEPRPGRRFADVERNI
IIPDVIVRKAGRANGRDGQHNFIVQLNPDVMPRLRVHDIYAGALRGHKGGEGHQGMQQRLQEARWFIKNIQQRFDTILRV
SRAIVERQKNFFTHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPIGTYELKYFFGSGLGTETGGNASSTAVRAL
IKQFVSAENPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=1064750 ACHMXC_RS05900 WP_108498276.1 1271634..1273235(-) (rpoN/rpoN1) [Acidovorax delafieldii strain UC81_30]
ATGAAGCCTGGACTGTCGCTGCGCGTCTCGCAGCACCTTGCATTGACCCCCCAGCTCCAGCAGTCCATCCGGCTGCTGCA
GTTGTCCACGCTCGAACTCTCGCAGGAAGTCGAGCAGATGCTCGACGAAAACCCGTTCCTCGAACGCAATGCCGACGAAG
CCCCGCGCGAGGAGTTTGGCCTGGAAGCCGCCGACACGCCCGCGCAGGCCGACGACTACAGCGCCGATGATGCTATGTTT
TCAGGAGCTGTCAGCGCAAGCAGTACTAGCGCTGAGAGCCAAAACGAGGCTGAAATCCCCAGTGGAGGCGCCGACGAGCC
CGACTGGAGCGGTGACGGCACCGTCGAGATGGCGCCCAACGACGCCGAGTGGGGTGGCGACGCCCCGGCGCGCACCCGCA
ACGATGCGGAAGGCGACGAGGCCGACGCCACCGAGCTGGCCCGTTCGCACGAATCGCTCACCGCCTTTTTGCACCGCCAG
GCACTGTCGCTGAGGCTGTCTGAAGTGGACACTGCCGCGCTGCGTTTTCTCATTGAATCGCTGAACGACGACGGCTACCT
CGAAGAGCCTCTGGAAGAACTCGCTACCAGCCTGGCCGGGCCCGACGACCTGGAACAGATCGAAGAACTGGTGCACCGCT
TCACCGTGGCCCAACGTCTGCTGCAGAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCAACCTGGCCGAATGCCTGACGCTG
CAGCTCACCGCGCTGGCGGCCGACGAAGACAGCGACGCCGACCCCGAGACCGTGCAGACCGCGCTGCGCATCTGTCAGCA
GCCGCTGGAGATGCTGGCCCGCCGCGACATCCGCCGCCTGACGCAGCTGTGCAGCGAAGGTGGCGTGGCCGGAGAAGAAC
GCACCCGATCTGCCATGGCACTCATCGCCCGCCTGGAGCCTCGCCCTGGCCGCCGTTTTGCCGACGTAGAGCGCAACATC
ATCATCCCCGACGTCATCGTGCGCAAAGCCGGCCGTGCCAACGGGCGGGACGGCCAGCACAACTTCATCGTGCAGCTCAA
CCCCGATGTGATGCCCCGTCTGCGCGTGCACGACATCTACGCCGGCGCGCTGCGTGGCCATAAGGGCGGCGAAGGCCACC
AGGGCATGCAGCAGCGCCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGTTTTGACACCATCCTGCGCGTC
TCGCGCGCCATCGTCGAGCGGCAGAAGAACTTCTTCACCCACGGCGAACTGGCCATGCGCCCCCTGGTGCTGCGCGACAT
CGCCGACGAACTGGGTCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCGATCGGTACCT
ACGAGCTGAAGTACTTCTTTGGCTCTGGCCTGGGCACCGAGACCGGTGGCAATGCGTCCAGCACGGCCGTGCGCGCACTG
ATCAAGCAGTTCGTGTCCGCCGAAAACCCGGCCAAGCCGCTGTCCGACAGCCAGATCGCCGAGATGCTCAAGGAGCAGGG
CATCGAATGCGCACGCCGCACCGTTGCCAAGTACCGCGAGGCGCTCAAGATCGCGCCGGCGAATCTCCGCAAAGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-360)

Sigma-54, DNA binding domain (Sigma54_DBD)

(376-531)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R7NZI6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.574

100

0.537

Multiple sequence alignment