Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHMXA_RS06540 Genome accession   NZ_CP171978
Coordinates   1379282..1380871 (+) Length   529 a.a.
NCBI ID   WP_395319466.1    Uniprot ID   -
Organism   Variovorax sp. UC74_104     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1374282..1385871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXA_RS06515 (ACHMXA_06515) argS 1374313..1375998 (+) 1686 WP_395319457.1 arginine--tRNA ligase -
  ACHMXA_RS06520 (ACHMXA_06520) - 1376010..1376786 (+) 777 WP_395319459.1 SPOR domain-containing protein -
  ACHMXA_RS06525 (ACHMXA_06525) - 1376944..1377591 (+) 648 WP_176665988.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACHMXA_RS06530 (ACHMXA_06530) lptA 1377744..1378469 (+) 726 WP_395319462.1 lipopolysaccharide transport periplasmic protein LptA -
  ACHMXA_RS06535 (ACHMXA_06535) lptB 1378466..1379260 (+) 795 WP_395319464.1 LPS export ABC transporter ATP-binding protein -
  ACHMXA_RS06540 (ACHMXA_06540) rpoN/rpoN1 1379282..1380871 (+) 1590 WP_395319466.1 RNA polymerase factor sigma-54 Machinery gene
  ACHMXA_RS06545 (ACHMXA_06545) - 1380998..1382572 (+) 1575 WP_395319468.1 phospholipase D family protein -
  ACHMXA_RS06550 (ACHMXA_06550) - 1382583..1383227 (-) 645 WP_176665993.1 LysE family translocator -
  ACHMXA_RS06555 (ACHMXA_06555) - 1383268..1383636 (-) 369 WP_395319470.1 hypothetical protein -
  ACHMXA_RS06560 (ACHMXA_06560) - 1383647..1384294 (-) 648 WP_176665994.1 YceI family protein -
  ACHMXA_RS06565 (ACHMXA_06565) - 1384374..1384964 (-) 591 WP_395321240.1 YceI family protein -
  ACHMXA_RS06570 (ACHMXA_06570) - 1384967..1385560 (-) 594 WP_395319473.1 cytochrome b -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58203.24 Da        Isoelectric Point: 4.6284

>NTDB_id=1064713 ACHMXA_RS06540 WP_395319466.1 1379282..1380871(+) (rpoN/rpoN1) [Variovorax sp. UC74_104]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDTADAPVPRDDGGEGEGDF
ASPAASSSTSSSAESASTADSDGPAAEISEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESLQ
SFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPAGVGARDLG
ECLSIQLRAMASDDETEEEELVRKTALAICKQPMELLARRDFKRLATLTRSSEELVRSALQVIARLEPKPGRRFVDVERN
VIIPDVIVTKTGRGTNVKFRVMLNPDVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VGSESVKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1064713 ACHMXA_RS06540 WP_395319466.1 1379282..1380871(+) (rpoN/rpoN1) [Variovorax sp. UC74_104]
ATGAAGCAAGGGCTATCCCTTCGCGTTTCGCAGCATCTGGCGCTCACGCCGCAGCTGCAGCAGTCGATCAGGCTGCTGCA
GCTCTCCACGCTCGAACTGAGCCAGGAGGTGGAGCAGATGCTCGACGAGAACCCGTTCCTCGAGCGCACCGCGGAAGAAG
CCGCGCGCGAGGAATTCGGGCTCGACACGGCCGACGCGCCGGTCCCCCGCGATGACGGCGGCGAAGGCGAGGGCGACTTC
GCCTCCCCGGCCGCCAGCAGCAGCACGAGTTCGTCCGCCGAGAGCGCCTCCACCGCTGACTCCGACGGCCCCGCCGCCGA
GATCTCCGAGCGCGAGCCCGACTGGGAGGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAATGGGGCAGCGACG
CGCCCGCGCGCCAGAACAACCTGGGCGACGACGAGCGCGCCGACGCCACCGAGCTCGCGCGCAGCCAGGAGTCGCTGCAA
TCCTTCCTGCACCGCCAGGCGCTGAGCCTGCGCCTGAACGAGAACGACAGCGCCGCGCTGCGCTTCCTGATCGAATCGCT
CAACGACGACGGCTACCTCGAGGACTCGCTGCCCGCGCTGGCCTCGGGCCTGGCCGGCGACGACAACGACCAGTTCGACG
ACCTGGTGCATCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCCGCTGGCGTGGGTGCGCGCGACCTCGGC
GAGTGCCTGAGCATCCAGCTGCGCGCCATGGCCAGCGACGACGAGACCGAGGAAGAGGAACTGGTCCGCAAGACGGCGCT
GGCCATCTGCAAGCAGCCCATGGAGCTGCTCGCGCGCCGCGACTTCAAGCGCCTGGCCACGCTCACCCGCAGCAGCGAGG
AGCTGGTGCGCTCGGCGCTGCAGGTCATCGCGCGGCTGGAGCCCAAGCCGGGCCGGCGCTTCGTCGACGTCGAGCGCAAC
GTCATCATTCCGGACGTGATCGTCACCAAGACCGGCCGCGGCACCAACGTGAAGTTCCGCGTGATGCTCAACCCCGACGT
GATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGCGCGCTGAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGTCGC
AGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCCATC
GTCGAGCGGCAGAAGAGCTACTTCGTGCACGGCGAGCTCGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
GGGCCTACACGAATCGACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACGGTCGAGCTCAAGT
ACTTCTTCGGCTCGGCGCTGGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCGGTGCGCGCGCTCATCAAGCAGTTC
GTCGGCTCCGAGAGCGTGAAGAAGCCGCTGTCGGACAGCCAGATCTCCGAGATGCTGAAGGAGCAGGGCATCGAGTGCGC
GCGCCGCACCGTCGCCAAGTACCGCGAGGCGCTGCGCATCGCGCCCGCCAACCTGCGCAAAGCCTTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.075

100

0.522

Multiple sequence alignment