Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHFCC_RS00815 Genome accession   NZ_CP171806
Coordinates   164912..166423 (-) Length   503 a.a.
NCBI ID   WP_395140063.1    Uniprot ID   -
Organism   Schlegelella aquatica strain HS-12-14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 159912..171423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFCC_RS00795 (ACHFCC_00795) - 160887..161639 (+) 753 WP_395140055.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  ACHFCC_RS00800 (ACHFCC_00800) - 161837..162037 (-) 201 WP_395140057.1 CopG family ribbon-helix-helix protein -
  ACHFCC_RS00805 (ACHFCC_00805) - 162160..163500 (+) 1341 WP_395140059.1 PilC/PilY family type IV pilus protein -
  ACHFCC_RS00810 (ACHFCC_00810) - 163582..164883 (-) 1302 WP_395140061.1 class I SAM-dependent RNA methyltransferase -
  ACHFCC_RS00815 (ACHFCC_00815) rpoN/rpoN1 164912..166423 (-) 1512 WP_395140063.1 RNA polymerase factor sigma-54 Machinery gene
  ACHFCC_RS00820 (ACHFCC_00820) lptB 166526..167302 (-) 777 WP_395140065.1 LPS export ABC transporter ATP-binding protein -
  ACHFCC_RS00825 (ACHFCC_00825) lptA 167299..167895 (-) 597 WP_395142598.1 lipopolysaccharide transport periplasmic protein LptA -
  ACHFCC_RS00830 (ACHFCC_00830) - 167948..168596 (-) 649 Protein_165 thiol:disulfide interchange protein DsbA/DsbL -
  ACHFCC_RS00835 (ACHFCC_00835) - 168705..168806 (-) 102 WP_395142600.1 SPOR domain-containing protein -
  ACHFCC_RS00840 (ACHFCC_00840) - 168887..169315 (-) 429 Protein_167 hypothetical protein -
  ACHFCC_RS00845 (ACHFCC_00845) - 169373..169750 (-) 378 WP_395140067.1 DALR anticodon-binding domain-containing protein -
  ACHFCC_RS00850 (ACHFCC_00850) argS 169642..171060 (-) 1419 WP_395140069.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55613.07 Da        Isoelectric Point: 4.7002

>NTDB_id=1064281 ACHFCC_RS00815 WP_395140063.1 164912..166423(-) (rpoN/rpoN1) [Schlegelella aquatica strain HS-12-14]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEPEEDPAGEAAPTAAGPERTPASETLAAEAESG
DGFEAPEAPSMDSVEFGATEREDWENGTERDDFDGIRETPAPRNGANDDELDPIDRASPGISLQDHLRQQLMGMRLAPVD
AAAVMVLIESLDEDGYLADSLEEIAERLAGDDAEAREELLERLQCALKWLQSLEPTGVGARNLSECLCLQLQALPRSEAQ
VVATLICKHHLETLARRDIKKLMASTGAGEETIKEAQSLIVSLEPKPGRPFTRAEANVVVPDVIVQKVGRQWKVVLNPDV
MPRLRINDLYAQALKQQRAHEGAQALSARLQEARWFMKNIQQRFETIQRVAQAIVERQKNFFTHGEIAMKPLVLREIADE
LGLHESTISRVTTAKYMATPFGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVASEDPKKPLSDSQISEMLGEQGIQV
ARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=1064281 ACHFCC_RS00815 WP_395140063.1 164912..166423(-) (rpoN/rpoN1) [Schlegelella aquatica strain HS-12-14]
ATGAAGCCTTCCCTGCAGGTCCGGCTGTCGCAGCACCTGGCGCTCACGCCCCAGCTGCAGCAGTCGATCCGCCTGCTGCA
ACTCTCCACGCTCGAGCTGCACCAGGAAGTCGAGCAGATGCTGGAGCAGAACCCCTTCCTCGAGCCGGAGGAAGACCCTG
CTGGCGAAGCCGCGCCGACCGCCGCCGGCCCCGAACGGACCCCCGCCTCCGAAACCCTCGCGGCCGAGGCCGAGAGCGGC
GACGGTTTCGAGGCCCCCGAAGCGCCGAGCATGGACAGCGTCGAGTTCGGCGCCACCGAGCGCGAGGACTGGGAGAACGG
CACTGAGCGCGACGATTTCGACGGCATCCGCGAAACGCCGGCGCCACGCAACGGGGCGAACGACGACGAGCTGGACCCGA
TCGACCGGGCCTCCCCCGGCATCAGCCTGCAAGACCACCTGCGCCAGCAGCTCATGGGGATGCGGCTGGCGCCGGTGGAT
GCGGCCGCGGTGATGGTGCTGATCGAGTCGCTCGACGAAGACGGCTACCTCGCCGACTCGCTGGAGGAGATCGCCGAGCG
TCTGGCGGGCGACGACGCCGAGGCGCGCGAGGAGTTGCTCGAGCGGCTGCAATGCGCGCTCAAGTGGTTGCAGAGCCTGG
AGCCCACCGGGGTGGGCGCGCGCAACCTGTCCGAATGCCTGTGCCTGCAGTTGCAGGCGCTGCCGCGCAGCGAGGCGCAG
GTGGTGGCGACCCTCATCTGCAAGCACCACCTCGAGACGCTCGCGCGGCGCGACATCAAGAAGCTGATGGCATCCACCGG
GGCCGGCGAGGAGACGATCAAGGAAGCCCAGAGCCTCATCGTCTCGCTCGAGCCCAAACCGGGGCGCCCCTTCACGCGTG
CCGAGGCCAACGTCGTCGTGCCCGATGTCATCGTGCAGAAGGTCGGGCGGCAATGGAAGGTGGTGCTCAACCCCGACGTG
ATGCCCCGGCTTCGCATCAACGACCTCTACGCCCAGGCGCTCAAGCAGCAGCGCGCCCACGAGGGCGCGCAGGCGTTGAG
CGCGCGGCTGCAGGAGGCGCGCTGGTTCATGAAGAACATCCAGCAGCGCTTCGAGACCATCCAGCGCGTGGCGCAGGCCA
TCGTCGAGCGGCAGAAGAACTTCTTCACGCACGGCGAGATCGCGATGAAGCCGCTCGTGCTGCGCGAGATCGCCGACGAA
CTCGGTCTGCACGAATCGACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACACCCTTCGGCACGTTCGAGCTGAA
GTACTTCTTTGGCTCGTCACTCAACACCGAGGCGGGAGGCAATGCCTCCAGCACCGCCGTGCGCGCGCTCATCAAGCAGC
TGGTGGCCTCCGAGGACCCGAAGAAGCCGCTGTCGGACAGCCAGATCAGCGAGATGCTCGGCGAGCAGGGCATCCAGGTG
GCGCGCCGCACCGTGGCCAAGTACCGGGAGGCGTTGAAGATCGCACCCGCCAATTTGCGCAAGGCGTTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-330)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

56.117

100

0.575

Multiple sequence alignment