Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACGLYC_RS08995 Genome accession   NZ_CP171691
Coordinates   1823336..1823770 (+) Length   144 a.a.
NCBI ID   WP_032866024.1    Uniprot ID   -
Organism   Bacillus velezensis strain K12     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1818336..1828770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGLYC_RS08990 (ACGLYC_08990) - 1821836..1823047 (-) 1212 WP_007409852.1 cytochrome P450 -
  ACGLYC_RS08995 (ACGLYC_08995) nucA/comI 1823336..1823770 (+) 435 WP_032866024.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACGLYC_RS09000 (ACGLYC_09000) - 1823830..1824585 (+) 756 WP_003154084.1 YoaK family protein -
  ACGLYC_RS09005 (ACGLYC_09005) - 1824619..1824981 (-) 363 WP_007410383.1 hypothetical protein -
  ACGLYC_RS09010 (ACGLYC_09010) - 1825174..1826502 (-) 1329 WP_032866023.1 S8 family peptidase -
  ACGLYC_RS09015 (ACGLYC_09015) - 1826681..1826914 (+) 234 WP_015239897.1 hypothetical protein -
  ACGLYC_RS09020 (ACGLYC_09020) - 1827170..1827877 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  ACGLYC_RS09025 (ACGLYC_09025) - 1827937..1828389 (+) 453 WP_032866022.1 OsmC family protein -
  ACGLYC_RS09030 (ACGLYC_09030) - 1828403..1828756 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=1063372 ACGLYC_RS08995 WP_032866024.1 1823336..1823770(+) (nucA/comI) [Bacillus velezensis strain K12]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHTEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1063372 ACGLYC_RS08995 WP_032866024.1 1823336..1823770(+) (nucA/comI) [Bacillus velezensis strain K12]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCTATTTCGGCAGGTCATACTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCATCAGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment