Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACF0HT_RS11665 Genome accession   NZ_CP171612
Coordinates   2421459..2422829 (-) Length   456 a.a.
NCBI ID   WP_017723050.1    Uniprot ID   A0A2T4QHV3
Organism   Staphylococcus xylosus strain 14BME18     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2416459..2427829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0HT_RS11650 (ACF0HT_11650) cysE 2417449..2418099 (-) 651 WP_037557109.1 serine O-acetyltransferase -
  ACF0HT_RS11655 (ACF0HT_11655) gltX 2418443..2419897 (-) 1455 WP_042363308.1 glutamate--tRNA ligase -
  ACF0HT_RS11660 (ACF0HT_11660) - 2420320..2421378 (-) 1059 WP_042363309.1 PIN/TRAM domain-containing protein -
  ACF0HT_RS11665 (ACF0HT_11665) radA 2421459..2422829 (-) 1371 WP_017723050.1 DNA repair protein RadA Machinery gene
  ACF0HT_RS11670 (ACF0HT_11670) - 2423172..2425634 (-) 2463 WP_042363310.1 ATP-dependent Clp protease ATP-binding subunit -
  ACF0HT_RS11675 (ACF0HT_11675) - 2425648..2426658 (-) 1011 WP_029377943.1 protein arginine kinase -
  ACF0HT_RS11680 (ACF0HT_11680) - 2426651..2427202 (-) 552 WP_394523594.1 UvrB/UvrC motif-containing protein -
  ACF0HT_RS11685 (ACF0HT_11685) - 2427219..2427680 (-) 462 WP_029377945.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50406.90 Da        Isoelectric Point: 7.2146

>NTDB_id=1062350 ACF0HT_RS11665 WP_017723050.1 2421459..2422829(-) (radA) [Staphylococcus xylosus strain 14BME18]
MAKKKVIFECMACGYQSPKWMGKCPNCGGWNQMEEIVEQKAASPKHGVRSRDNISKVQKLNDIKHETTPRVKTNSEEFNR
VLGGGIVNGSLVLIGGDPGIGKSTLLLQICAALSQSKNVLYITGEESLNQTKLRAERLDEDSSQLNVFAETDLEVIHETV
KQLKPDLLVVDSIQTIFHPEISSAPGSVSQVRESTQSLMNIAKQLNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMQQTGLKGVKNPSEMFLEERSTNVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLGIVVATASSFKDLTVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAEKLGFKRVIIPESNIGGWQFPNDIKVIGVKNVHEALKYALTKQ

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1062350 ACF0HT_RS11665 WP_017723050.1 2421459..2422829(-) (radA) [Staphylococcus xylosus strain 14BME18]
TTGGCTAAAAAGAAAGTAATTTTTGAATGTATGGCCTGTGGCTACCAGTCTCCAAAGTGGATGGGGAAATGCCCCAATTG
TGGTGGCTGGAATCAAATGGAAGAAATAGTCGAACAAAAGGCCGCGAGTCCAAAACATGGCGTACGCAGTCGAGACAATA
TTTCTAAAGTACAAAAATTAAATGACATAAAACATGAAACGACGCCACGAGTAAAAACAAACTCTGAAGAATTTAATAGA
GTGCTTGGCGGCGGTATAGTAAATGGTTCGCTTGTATTGATCGGTGGCGATCCAGGTATTGGTAAATCTACATTACTCTT
ACAAATTTGTGCAGCATTATCACAATCTAAAAATGTGCTGTATATTACAGGTGAGGAATCGTTAAATCAAACGAAATTAA
GAGCAGAAAGGTTAGACGAGGACTCAAGTCAATTAAATGTGTTTGCTGAAACAGATTTAGAAGTTATACATGAAACAGTT
AAGCAACTCAAACCAGATTTACTTGTAGTAGACTCAATCCAAACTATTTTTCATCCAGAAATTAGTTCGGCACCAGGTTC
TGTGTCGCAAGTTAGAGAAAGTACGCAAAGTTTAATGAATATAGCTAAACAATTGAATATAGCTACATTTATAGTGGGAC
ATGTTACGAAAGAAGGCCAAATAGCTGGCCCGCGTTTACTTGAACACATGGTGGATACAGTTCTATACTTTGAAGGGGAC
GAGCATCATGCGTATCGTATTTTAAGAGCAGTTAAAAACCGTTTTGGCTCTACGAATGAGATGGGTATCTTTGAAATGCA
ACAAACTGGATTAAAAGGAGTAAAAAATCCGTCGGAAATGTTCCTTGAAGAACGTTCGACTAATGTACCTGGGTCAACCA
TTGTTTCTACGATGGAAGGCACGAGGCCATTACTTATAGAAGTACAAGCGTTAGTGACGCCAACTACATTCAATAATCCA
AGACGTATGGCGACAGGTATCGATCATAATCGATTGAGTTTGCTCATGGCAGTGCTAGAAAAGAAAGAGAATTATTTGCT
ACAACAACAGGATGCTTATATTAAAGTAGCAGGTGGTGTACGTTTAACAGAACCTGCAGTAGATTTAGGTATTGTTGTTG
CGACGGCTTCAAGTTTTAAAGATTTAACAGTTGATGGTTTAGACTGCTTTATTGGAGAAGTAGGACTCACAGGGGAAGTT
AGGCGTGTTTCGCGTATAGAGCAACGTGTACAAGAAGCGGAAAAATTAGGGTTTAAGCGCGTAATTATTCCAGAATCAAA
TATTGGCGGATGGCAATTTCCAAATGACATTAAGGTTATTGGTGTTAAGAATGTCCATGAAGCATTAAAATATGCACTAA
CAAAACAGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-219)

Subunit ChlI of Mg-chelatase (ChlI)

(356-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4QHV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.351

100

0.654
  radA Streptococcus pneumoniae Rx1

60

98.684

0.592
  radA Streptococcus pneumoniae D39

60

98.684

0.592
  radA Streptococcus pneumoniae R6

60

98.684

0.592
  radA Streptococcus pneumoniae TIGR4

60

98.684

0.592
  radA Streptococcus mitis NCTC 12261

60

98.684

0.592
  radA Streptococcus mitis SK321

60

98.684

0.592

Multiple sequence alignment