Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACE1MM_RS12570 Genome accession   NZ_CP171355
Coordinates   2645469..2646800 (+) Length   443 a.a.
NCBI ID   WP_082062952.1    Uniprot ID   A0AAJ4ZIU9
Organism   Ralstonia mannitolilytica strain SY001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2640469..2651800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1MM_RS12545 (ACE1MM_12545) - 2640813..2641073 (-) 261 WP_045785720.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ACE1MM_RS12550 (ACE1MM_12550) coaD 2641124..2641627 (-) 504 WP_045785719.1 pantetheine-phosphate adenylyltransferase -
  ACE1MM_RS12555 (ACE1MM_12555) rsmD 2641822..2642478 (-) 657 WP_045785718.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACE1MM_RS12560 (ACE1MM_12560) - 2642560..2643912 (-) 1353 WP_062739106.1 pitrilysin family protein -
  ACE1MM_RS12565 (ACE1MM_12565) - 2643912..2645408 (-) 1497 WP_045785716.1 pitrilysin family protein -
  ACE1MM_RS12570 (ACE1MM_12570) pilA 2645469..2646800 (+) 1332 WP_082062952.1 signal recognition particle-docking protein FtsY Machinery gene
  ACE1MM_RS12575 (ACE1MM_12575) - 2647053..2647415 (+) 363 WP_115043821.1 PAAR domain-containing protein -
  ACE1MM_RS12580 (ACE1MM_12580) - 2647427..2648419 (+) 993 WP_238587591.1 M35 family metallo-endopeptidase -
  ACE1MM_RS12585 (ACE1MM_12585) - 2648400..2648834 (+) 435 WP_147282523.1 hypothetical protein -
  ACE1MM_RS12590 (ACE1MM_12590) - 2648881..2649033 (+) 153 WP_157773986.1 hypothetical protein -
  ACE1MM_RS12595 (ACE1MM_12595) - 2649096..2649794 (+) 699 WP_062739108.1 hypothetical protein -
  ACE1MM_RS12600 (ACE1MM_12600) maiA 2649937..2650581 (-) 645 WP_062739109.1 maleylacetoacetate isomerase -
  ACE1MM_RS12605 (ACE1MM_12605) - 2650608..2651309 (-) 702 WP_062739110.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 46334.23 Da        Isoelectric Point: 5.0723

>NTDB_id=1060847 ACE1MM_RS12570 WP_082062952.1 2645469..2646800(+) (pilA) [Ralstonia mannitolilytica strain SY001]
MFSFWKKRKAEPQPAAESALAAAPEPAQAPTPATAVPPPAADTPAAVVTPDASASTPTPPATEDARPGWLSRLGFGGAPA
PEPAAPQAPASAPVETQVPTPAAAPTPATPVAPVAVPDTPAAPPALDMQAGDIETVPTPAIIEQGRKGWMSRLRSGLSKT
SKNLTTLFVGVKVDEALFEELETALLMADAGVDATEYLLGELRRRIKAERIETAEGVKTALRDLLVELLHPLEKTMVLGR
EQPMVIMIAGVNGAGKTTSIGKLCKHFQTYGQSVLLAAGDTFRAAAREQLVIWGERNNVTVVAQESGDPAAVIYDAVNAA
RARGIDIVMADTAGRLPTQLHLMEELKKVRRVIGKAMATAPHETLLVIDGNTGQNALAQLKAFDDALGLTGLIVTKLDGT
AKGGILAAIARQRPVPVYFIGVGEQVEDLQPFSAREFADALLG

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=1060847 ACE1MM_RS12570 WP_082062952.1 2645469..2646800(+) (pilA) [Ralstonia mannitolilytica strain SY001]
ATGTTTAGTTTCTGGAAAAAGCGCAAGGCGGAGCCCCAACCGGCCGCCGAATCCGCCCTCGCAGCCGCTCCCGAGCCCGC
ACAGGCGCCGACGCCCGCTACGGCCGTACCCCCCCCTGCGGCAGACACACCGGCCGCGGTTGTCACGCCCGACGCCAGCG
CCTCCACGCCAACGCCGCCAGCCACGGAAGACGCCCGCCCGGGCTGGCTCTCGCGCCTGGGCTTTGGCGGCGCTCCGGCA
CCCGAACCGGCCGCCCCGCAAGCGCCGGCATCCGCGCCCGTCGAAACGCAGGTTCCGACACCCGCCGCCGCACCAACGCC
GGCCACGCCAGTCGCACCGGTGGCCGTGCCCGACACGCCAGCCGCGCCGCCCGCGCTCGACATGCAGGCCGGCGATATCG
AAACCGTGCCTACGCCGGCCATCATCGAACAGGGACGCAAGGGCTGGATGTCACGCCTGCGCTCAGGCCTGTCGAAGACG
AGCAAGAACCTCACGACGCTGTTCGTCGGCGTGAAGGTGGACGAAGCGCTGTTCGAAGAACTCGAGACCGCGCTGCTGAT
GGCCGACGCCGGCGTCGATGCCACCGAATACCTGCTCGGCGAACTGCGCCGCCGCATCAAGGCCGAGCGCATCGAAACCG
CCGAGGGCGTCAAGACCGCCCTGCGCGACCTGCTCGTCGAGCTGCTGCACCCGCTGGAAAAGACCATGGTGCTCGGCCGC
GAACAGCCGATGGTCATCATGATTGCGGGCGTCAACGGTGCCGGCAAGACGACCAGCATCGGCAAGCTGTGCAAGCACTT
CCAGACCTATGGCCAGTCGGTGCTGCTGGCAGCAGGCGACACCTTCCGCGCCGCCGCGCGCGAGCAGCTCGTCATCTGGG
GCGAGCGCAACAACGTGACGGTCGTCGCGCAGGAATCCGGCGACCCGGCCGCGGTCATCTACGACGCAGTCAACGCCGCA
CGTGCACGCGGCATCGACATCGTCATGGCCGACACCGCGGGCCGCCTGCCGACGCAGTTGCACCTGATGGAAGAGCTGAA
GAAGGTGCGCCGCGTGATCGGCAAGGCCATGGCGACGGCCCCACACGAAACGCTGCTCGTGATCGACGGCAACACCGGCC
AGAACGCGCTTGCGCAGCTGAAGGCGTTCGACGATGCGCTGGGCCTCACGGGGCTGATCGTCACCAAGCTCGATGGCACC
GCCAAGGGCGGCATCCTCGCTGCGATCGCAAGGCAGCGGCCGGTGCCGGTGTACTTCATCGGCGTTGGCGAGCAGGTGGA
GGATCTGCAGCCGTTTTCTGCGCGCGAATTTGCGGATGCGCTGTTGGGATAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(243-443)

SRP54-type protein, helical bundle domain (SRP54_N)

(162-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.546

100

0.49

Multiple sequence alignment