Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACE1MM_RS12455 Genome accession   NZ_CP171355
Coordinates   2623482..2624981 (+) Length   499 a.a.
NCBI ID   WP_062739101.1    Uniprot ID   A0AAJ5D4K4
Organism   Ralstonia mannitolilytica strain SY001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2618482..2629981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1MM_RS12430 (ACE1MM_12430) - 2619711..2620694 (+) 984 WP_045785739.1 SIS domain-containing protein -
  ACE1MM_RS12435 (ACE1MM_12435) - 2620711..2621295 (+) 585 WP_062739099.1 HAD family hydrolase -
  ACE1MM_RS12440 (ACE1MM_12440) lptC 2621300..2621932 (+) 633 WP_045785737.1 LPS export ABC transporter periplasmic protein LptC -
  ACE1MM_RS12445 (ACE1MM_12445) lptA 2621991..2622557 (+) 567 WP_062739100.1 lipopolysaccharide transport periplasmic protein LptA -
  ACE1MM_RS12450 (ACE1MM_12450) lptB 2622586..2623374 (+) 789 WP_045785735.1 LPS export ABC transporter ATP-binding protein -
  ACE1MM_RS12455 (ACE1MM_12455) rpoN/rpoN1 2623482..2624981 (+) 1500 WP_062739101.1 RNA polymerase factor sigma-54 Machinery gene
  ACE1MM_RS12460 (ACE1MM_12460) hpf 2625033..2625386 (+) 354 WP_045785733.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACE1MM_RS12465 (ACE1MM_12465) ptsN 2625729..2626184 (+) 456 WP_004633390.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACE1MM_RS12470 (ACE1MM_12470) hprK 2626255..2627229 (+) 975 WP_045785732.1 HPr(Ser) kinase/phosphatase -
  ACE1MM_RS12475 (ACE1MM_12475) - 2627416..2627739 (+) 324 WP_045785731.1 PsiF family protein -
  ACE1MM_RS12480 (ACE1MM_12480) rapZ 2627834..2628724 (+) 891 WP_062739102.1 RNase adapter RapZ -
  ACE1MM_RS12485 (ACE1MM_12485) - 2628817..2629470 (+) 654 WP_045785729.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55327.78 Da        Isoelectric Point: 4.9066

>NTDB_id=1060846 ACE1MM_RS12455 WP_062739101.1 2623482..2624981(+) (rpoN/rpoN1) [Ralstonia mannitolilytica strain SY001]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAPNGTDSTDAPETSYDSSADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSMR
DKGLVIFLIESLDDEGYLSGTLDEILADLPSELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLESPAKTLAL
TIVNHHLELLAARDYTRLKKALSVDEPALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTSAGWMAQLNPEVMPRL
RINDMYAQILRSSRGEAGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1060846 ACE1MM_RS12455 WP_062739101.1 2623482..2624981(+) (rpoN/rpoN1) [Ralstonia mannitolilytica strain SY001]
ATGAAACAGTCGCTCCAGCTTCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTCCAGCAGGAAGTCGAACAGGCTCTGACCGAAAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACGTCCGCGCGCATCGGCGCAGATGGCTCCGTCAACGCGCTGCAAAGCAGCAGCGCCACACCGCTGCCCACCGAA
TCGCCACCGCCCGCGCCCAACGGCACGGACAGCACCGACGCTCCCGAAACCAGCTACGACAGCAGTGCCGACTTCGACAG
CGATTACGGATCCGACCGCTCCGACTGGAGCCTCGACGACTTCGCCCGCAAGCCGCAATCGGACGAAGACGACCGCGCGC
CCATGCAGCTGCGTGAAGCCGAGCCGACGCTGCGCGAATTCCTCATGGAGCAGCTCGCGCCGCTCAAGCTGTCGATGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTCGATGACGAAGGCTATCTCTCGGGCACACTCGACGAAATCCTCGC
CGATCTGCCGTCGGAACTCGAGGTCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGATCCGCCCG
GTATCGGCGCGCGCTCGGCCGCCGAATGCCTGTCGCTGCAGTTGCACCGGCTCGAATCACCGGCGAAGACGCTCGCGCTC
ACGATCGTCAATCACCATCTCGAACTGCTCGCCGCACGCGATTACACACGGCTGAAGAAGGCACTGTCCGTCGACGAGCC
GGCGCTCAAGGCGGCACACGAGCTCATTCGTTCATTGGCGCCGTTTCCAGGGCATGCGTTCGGCCGCGCAGAGGCCGACT
TTGTCGTGCCCGACGTCGTCGTGCGCAAGACCAGTGCGGGCTGGATGGCGCAGTTGAATCCCGAAGTGATGCCGCGTCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGTGAAGCCGGCGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGTTGGCTGATCAAGAACATCCAGCAGCGTTTCGATACGATCCTGCGTGTCTCGCAAGCCATTGTCGAGCGTC
AAAAGAGCTTCTTCACGCACGGTGAAATCGCCATGCGGCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGCACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCTGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTGAAGATCCCAGCAGTGAATCTGCGCAAGTCCTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

93.988

100

0.94

Multiple sequence alignment