Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACF0HW_RS16640 Genome accession   NZ_CP171208
Coordinates   3374112..3374549 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain HN11     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3369112..3379549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0HW_RS16625 - 3371970..3372332 (-) 363 WP_012116770.1 helix-turn-helix domain-containing protein -
  ACF0HW_RS16630 hxlA 3372564..3373199 (+) 636 WP_012116769.1 3-hexulose-6-phosphate synthase -
  ACF0HW_RS16635 hxlB 3373196..3373753 (+) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  ACF0HW_RS16640 nucA/comI 3374112..3374549 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACF0HW_RS16645 nin/comJ 3374570..3374968 (+) 399 WP_007609299.1 competence protein ComJ Regulator
  ACF0HW_RS16650 - 3375009..3376445 (-) 1437 WP_012116768.1 glycoside hydrolase family 1 protein -
  ACF0HW_RS16655 - 3376549..3376827 (-) 279 WP_263612330.1 YckD family protein -
  ACF0HW_RS16660 - 3376945..3377379 (-) 435 WP_012116767.1 RDD family protein -
  ACF0HW_RS16665 - 3377394..3378530 (-) 1137 WP_012116766.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1060511 ACF0HW_RS16640 WP_003156588.1 3374112..3374549(+) (nucA/comI) [Bacillus amyloliquefaciens strain HN11]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1060511 ACF0HW_RS16640 WP_003156588.1 3374112..3374549(+) (nucA/comI) [Bacillus amyloliquefaciens strain HN11]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment