Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACFN7Z_RS02595 Genome accession   NZ_CP171157
Coordinates   534524..535324 (+) Length   266 a.a.
NCBI ID   WP_004697674.1    Uniprot ID   -
Organism   Acinetobacter sp. P1332     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 529524..540324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFN7Z_RS02565 (ACFN7Z_02565) - 529740..530210 (+) 471 WP_213075192.1 transposase -
  ACFN7Z_RS02570 (ACFN7Z_02570) - 530292..531572 (+) 1281 WP_130235011.1 glycosyltransferase family 1 protein -
  ACFN7Z_RS02575 (ACFN7Z_02575) - 531765..532316 (+) 552 WP_130235010.1 phosphatase PAP2 family protein -
  ACFN7Z_RS02580 (ACFN7Z_02580) iscX 532407..532607 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  ACFN7Z_RS02585 (ACFN7Z_02585) ndk 532718..533149 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  ACFN7Z_RS02590 (ACFN7Z_02590) rlmN 533272..534507 (+) 1236 WP_130235009.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACFN7Z_RS02595 (ACFN7Z_02595) pilF 534524..535324 (+) 801 WP_004697674.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACFN7Z_RS02600 (ACFN7Z_02600) - 535315..536091 (+) 777 WP_130235008.1 helix-turn-helix domain-containing protein -
  ACFN7Z_RS02605 (ACFN7Z_02605) ispG 536110..537225 (+) 1116 WP_130235007.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACFN7Z_RS02610 (ACFN7Z_02610) hisS 537222..538517 (+) 1296 WP_062031308.1 histidine--tRNA ligase -
  ACFN7Z_RS02615 (ACFN7Z_02615) - 538545..539243 (+) 699 WP_005401704.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=1060365 ACFN7Z_RS02595 WP_004697674.1 534524..535324(+) (pilF) [Acinetobacter sp. P1332]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1060365 ACFN7Z_RS02595 WP_004697674.1 534524..535324(+) (pilF) [Acinetobacter sp. P1332]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGAGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTATTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCAGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCAGTTGGCGAGAAAAACTTAG
ATGCGCGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
TTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647

Multiple sequence alignment