Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACF3N8_RS01240 Genome accession   NZ_CP171119
Coordinates   265414..266784 (-) Length   456 a.a.
NCBI ID   WP_009383827.1    Uniprot ID   K9AXZ1
Organism   Staphylococcus massiliensis strain WGS1515     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 260414..271784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3N8_RS01220 (ACF3N8_01220) cysE 261782..262411 (-) 630 WP_193765462.1 serine O-acetyltransferase -
  ACF3N8_RS01225 (ACF3N8_01225) gltX 262678..264129 (-) 1452 WP_392450093.1 glutamate--tRNA ligase -
  ACF3N8_RS01230 (ACF3N8_01230) ispF 264164..264649 (-) 486 WP_009383829.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACF3N8_RS01235 (ACF3N8_01235) ispD 264642..265334 (-) 693 WP_392450095.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACF3N8_RS01240 (ACF3N8_01240) radA 265414..266784 (-) 1371 WP_009383827.1 DNA repair protein RadA Machinery gene
  ACF3N8_RS01245 (ACF3N8_01245) - 266914..269361 (-) 2448 WP_009383826.1 ATP-dependent Clp protease ATP-binding subunit -
  ACF3N8_RS01250 (ACF3N8_01250) - 269372..270379 (-) 1008 WP_238093920.1 protein arginine kinase -
  ACF3N8_RS01255 (ACF3N8_01255) - 270369..270902 (-) 534 WP_009383824.1 UvrB/UvrC motif-containing protein -
  ACF3N8_RS01260 (ACF3N8_01260) - 270915..271376 (-) 462 WP_009383823.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50347.98 Da        Isoelectric Point: 7.9980

>NTDB_id=1060214 ACF3N8_RS01240 WP_009383827.1 265414..266784(-) (radA) [Staphylococcus massiliensis strain WGS1515]
MAKKKVIYECMACGAQSPKWNGKCMNCGAWNQMEEVVERKSTPSKHGIKSDKKPAKVQKLSDVKQETTPRIQSGNDEFDR
VLGGGIVSGSLVLIGGDPGIGKSTLLLQMCAKLSKQKKVLYITGEESLNQTKLRAERLSEDSSELNVFSETDLEVIHETV
KHTEPDMIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKHSGLKAVRNPSEMFLEERSKNVPGSTIVATMEGTRPLLIEVQSLVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLAVIMAVASSFKNQAVDGLDCFIGEVGLTGEI
RRVSRIEQRVQEAEKLGFKRVIIPKTNLGGWDYPEGIKVIGVESTREALAIALQKA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1060214 ACF3N8_RS01240 WP_009383827.1 265414..266784(-) (radA) [Staphylococcus massiliensis strain WGS1515]
TTGGCTAAGAAAAAGGTGATTTACGAATGTATGGCGTGTGGAGCTCAATCGCCTAAATGGAACGGTAAATGCATGAACTG
TGGTGCTTGGAATCAAATGGAAGAAGTTGTAGAGCGTAAATCAACTCCAAGTAAGCATGGCATTAAAAGTGATAAGAAAC
CCGCTAAAGTACAAAAATTAAGTGACGTTAAGCAAGAAACAACGCCACGTATTCAATCTGGAAATGATGAATTCGACCGT
GTATTAGGTGGCGGTATTGTTTCAGGTTCGCTCGTGTTAATCGGTGGGGACCCTGGTATTGGTAAGTCGACGTTGCTTTT
ACAAATGTGCGCAAAACTTTCCAAACAAAAGAAAGTCTTATATATCACAGGTGAGGAATCTTTAAATCAAACGAAATTAC
GTGCTGAACGTTTATCTGAAGATTCAAGTGAGCTTAATGTGTTCAGTGAGACGGACCTTGAAGTGATTCATGAGACGGTT
AAACATACGGAACCGGATATGATTGTTGTGGATTCGATTCAAACGATTTATCATCCAGAAATCAGTTCGGCACCTGGTTC
TGTATCACAAGTGCGCGAGAGTACGCAAAGTTTAATGAATATCGCAAAGCAAATGAACATTGCTACATTTATCGTTGGTC
ACGTTACGAAAGAAGGTCAAATCGCTGGTCCAAGACTGCTTGAACATATGGTAGATACCGTGCTTTATTTCGAAGGTGAT
GAACATCACGCTTATCGAATCTTACGCGCTGTTAAAAACCGTTTCGGATCTACGAATGAAATGGGTATCTTCGAGATGAA
GCATAGTGGTCTCAAGGCGGTTCGTAATCCTTCTGAAATGTTTTTAGAAGAGCGTTCTAAAAATGTTCCTGGCTCAACGA
TTGTCGCGACTATGGAAGGTACGAGACCGTTACTGATTGAGGTACAATCACTCGTGACACCAACGACGTTTAATAATCCA
AGACGTATGGCTACGGGCATTGACCATAACCGTTTGAGTCTATTAATGGCCGTCCTTGAAAAGAAAGAAAATTATCTTCT
GCAGCAACAAGACGCTTACATTAAAGTAGCAGGTGGGGTCAGACTTACAGAACCTGCTGTCGATTTAGCGGTCATTATGG
CGGTTGCATCTAGTTTTAAGAACCAAGCAGTTGATGGGTTAGACTGCTTCATAGGTGAAGTCGGTCTAACAGGTGAAATC
AGACGTGTGTCTCGTATCGAGCAACGTGTTCAAGAAGCGGAGAAACTTGGCTTTAAACGTGTGATTATACCTAAGACAAA
CTTAGGTGGTTGGGACTATCCTGAAGGCATTAAAGTTATAGGTGTTGAATCGACAAGAGAAGCGTTAGCGATTGCTTTAC
AAAAAGCCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(356-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9AXZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

62.527

100

0.629
  radA Streptococcus pneumoniae Rx1

58.352

98.465

0.575
  radA Streptococcus pneumoniae D39

58.352

98.465

0.575
  radA Streptococcus pneumoniae R6

58.352

98.465

0.575
  radA Streptococcus pneumoniae TIGR4

58.352

98.465

0.575
  radA Streptococcus mitis NCTC 12261

58.352

98.465

0.575
  radA Streptococcus mitis SK321

58.352

98.465

0.575

Multiple sequence alignment