Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACF3ND_RS05490 Genome accession   NZ_CP171109
Coordinates   1183943..1185328 (+) Length   461 a.a.
NCBI ID   WP_394065786.1    Uniprot ID   -
Organism   Alcaligenes sp. WGS1538     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1178943..1190328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3ND_RS05470 (ACF3ND_05470) hpnD 1180214..1181077 (-) 864 WP_394065007.1 presqualene diphosphate synthase HpnD -
  ACF3ND_RS05475 (ACF3ND_05475) hpnC 1181084..1181953 (-) 870 WP_394065009.1 squalene synthase HpnC -
  ACF3ND_RS05480 (ACF3ND_05480) alr 1182075..1183205 (+) 1131 WP_394065011.1 alanine racemase -
  ACF3ND_RS05485 (ACF3ND_05485) - 1183224..1183940 (+) 717 WP_394065013.1 SDR family oxidoreductase -
  ACF3ND_RS05490 (ACF3ND_05490) radA 1183943..1185328 (+) 1386 WP_394065786.1 DNA repair protein RadA Machinery gene
  ACF3ND_RS05495 (ACF3ND_05495) - 1185336..1186103 (+) 768 WP_394065015.1 sulfite exporter TauE/SafE family protein -
  ACF3ND_RS05500 (ACF3ND_05500) - 1186113..1186832 (+) 720 WP_394065017.1 3-deoxy-D-manno-octulosonic acid kinase -
  ACF3ND_RS05505 (ACF3ND_05505) - 1186910..1188016 (+) 1107 WP_394065019.1 polyamine ABC transporter substrate-binding protein -
  ACF3ND_RS05510 (ACF3ND_05510) - 1188022..1189152 (+) 1131 WP_394065021.1 ABC transporter ATP-binding protein -
  ACF3ND_RS05515 (ACF3ND_05515) - 1189149..1190069 (+) 921 WP_394065023.1 ABC transporter permease subunit -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48842.15 Da        Isoelectric Point: 8.0066

>NTDB_id=1060091 ACF3ND_RS05490 WP_394065786.1 1183943..1185328(+) (radA) [Alcaligenes sp. WGS1538]
MATGARAGRKAKIVFVCNECGADTPKWAGKCPACGAWNSLQETVESPAAASSHRFASLAGSSPVQSLSDVQARELPRVPT
GIGEFDRVLGGGLVEGGVILIGGDPGVGKSTLLLQAMAALAQQTKVLYVTGEESAEQVALRARRLGVPTQGVDLLAEIRL
ESISQALQSHQPRIVVIDSIQTLYSDQLSAAPGSVSQVRECAAQLTRLAKQSGTAMIFIGHVTKDGTLAGPRVLEHIVDT
VLYFEGDTHSSFRMVRAFKNRFGAVNELGVFAMTEKGLKGVSNPSALFLSQHSQSVAGSCVMATQEGTRPLLVEIQALVD
SAHVPNPRRLSVGLESSRLAMLLAVMHRHAGVVTYDQDVFVNAVGGVKITEPAADLAVLLAILSSLNNRPLPRGLVVFGE
IGLAGEVRPAPRGQERLREAAKLGFGIALIPKSNAPRQPIEGLEIWAVDRVEDAVERLRNL

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1060091 ACF3ND_RS05490 WP_394065786.1 1183943..1185328(+) (radA) [Alcaligenes sp. WGS1538]
ATGGCCACAGGCGCGCGCGCCGGCCGCAAGGCCAAGATCGTCTTTGTCTGCAACGAATGCGGGGCCGACACGCCCAAGTG
GGCCGGCAAATGTCCGGCCTGCGGGGCCTGGAACAGCCTGCAGGAGACCGTGGAGTCGCCTGCGGCGGCTTCCTCGCACC
GTTTCGCTTCCCTGGCCGGTTCCAGTCCGGTGCAAAGTCTGTCCGACGTCCAGGCGCGCGAACTGCCGCGCGTGCCCACC
GGCATCGGCGAATTCGATCGCGTGCTGGGCGGCGGGCTGGTGGAAGGCGGCGTGATCCTGATCGGCGGCGACCCGGGCGT
GGGCAAGTCCACGCTGCTGCTGCAGGCCATGGCCGCCCTGGCCCAGCAGACCAAGGTCCTGTATGTTACCGGCGAAGAAT
CCGCCGAGCAGGTGGCCCTGCGGGCCCGGCGCCTGGGCGTGCCCACGCAGGGCGTGGACCTGCTGGCCGAGATCCGGCTG
GAGTCCATCAGCCAGGCCCTGCAGTCGCACCAGCCCCGCATCGTGGTCATCGACTCCATCCAGACGCTGTATTCGGACCA
GCTCAGCGCCGCGCCCGGTTCGGTGTCGCAGGTGCGCGAGTGCGCCGCACAGCTGACCCGCCTGGCCAAGCAGAGCGGCA
CCGCCATGATCTTCATCGGCCACGTCACCAAGGACGGCACGCTGGCCGGCCCGCGCGTGCTGGAGCACATCGTGGACACG
GTGCTGTATTTCGAAGGCGACACGCATTCCTCCTTCCGCATGGTGCGCGCCTTCAAGAACCGCTTTGGCGCGGTCAACGA
GCTGGGCGTGTTCGCCATGACGGAGAAAGGCCTCAAGGGCGTGAGCAACCCGTCCGCGCTGTTCTTGTCGCAGCATTCGC
AAAGCGTGGCCGGCTCCTGCGTCATGGCCACGCAGGAAGGCACGCGGCCCCTGCTGGTGGAGATCCAGGCGCTGGTGGAT
TCGGCCCACGTGCCCAATCCGCGCCGCCTGTCCGTGGGCCTGGAAAGCTCGCGGCTGGCCATGCTGCTGGCGGTCATGCA
TCGCCACGCGGGCGTGGTCACTTACGACCAGGACGTGTTCGTCAACGCCGTGGGCGGCGTCAAGATCACCGAGCCGGCCG
CCGACCTGGCCGTGCTGCTGGCCATCTTGTCGTCCCTGAACAACCGCCCCCTGCCGCGCGGGCTGGTGGTGTTCGGCGAG
ATCGGCCTGGCCGGCGAGGTCCGCCCGGCCCCGCGCGGGCAGGAGCGCCTGCGCGAAGCGGCCAAGCTGGGCTTCGGCAT
CGCGCTCATTCCCAAGAGCAATGCGCCGCGCCAGCCCATCGAGGGACTGGAGATCTGGGCCGTGGACCGGGTCGAGGACG
CGGTCGAGCGCCTGCGCAACCTGTAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(365-455)

AAA domain (AAA_25)

(88-226)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(15-42)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.899

98.482

0.482
  radA Streptococcus mitis SK321

45.695

98.265

0.449
  radA Streptococcus mitis NCTC 12261

45.575

98.048

0.447
  radA Streptococcus pneumoniae TIGR4

47.775

92.625

0.443
  radA Streptococcus pneumoniae Rx1

47.775

92.625

0.443
  radA Streptococcus pneumoniae D39

47.775

92.625

0.443
  radA Streptococcus pneumoniae R6

47.775

92.625

0.443

Multiple sequence alignment