Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACF3MZ_RS20390 Genome accession   NZ_CP171108
Coordinates   4500084..4501451 (-) Length   455 a.a.
NCBI ID   WP_394045806.1    Uniprot ID   -
Organism   Cohnella sp. WGS1546     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4495084..4506451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3MZ_RS20365 (ACF3MZ_20365) ispD 4495484..4496194 (-) 711 WP_394045796.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACF3MZ_RS20370 (ACF3MZ_20370) - 4496296..4497387 (-) 1092 WP_394045798.1 PIN/TRAM domain-containing protein -
  ACF3MZ_RS20375 (ACF3MZ_20375) - 4497579..4497974 (+) 396 WP_394045800.1 DUF1573 domain-containing protein -
  ACF3MZ_RS20380 (ACF3MZ_20380) pssA 4498103..4498852 (-) 750 WP_394045802.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  ACF3MZ_RS20385 (ACF3MZ_20385) disA 4498957..4500066 (-) 1110 WP_394045804.1 DNA integrity scanning diadenylate cyclase DisA -
  ACF3MZ_RS20390 (ACF3MZ_20390) radA 4500084..4501451 (-) 1368 WP_394045806.1 DNA repair protein RadA Machinery gene
  ACF3MZ_RS20395 (ACF3MZ_20395) clpC 4502035..4504482 (-) 2448 WP_394045808.1 ATP-dependent protease ATP-binding subunit ClpC -
  ACF3MZ_RS20400 (ACF3MZ_20400) - 4504519..4505583 (-) 1065 WP_394045810.1 protein arginine kinase -
  ACF3MZ_RS20405 (ACF3MZ_20405) - 4505600..4506112 (-) 513 WP_394045811.1 UvrB/UvrC motif-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49310.85 Da        Isoelectric Point: 7.0430

>NTDB_id=1060078 ACF3MZ_RS20390 WP_394045806.1 4500084..4501451(-) (radA) [Cohnella sp. WGS1546]
MAKVKIKFVCTQCGTESPKWMGKCPGCQEWNTMVEETETEVKALVGRSEQTRAKEKPRAIIHIESGQEPRIATGMAELNR
VLGGGLVPGSLLLVGGDPGIGKSTLLLQTSYSLSAQGLKVLYVSGEESVRQTKLRADRLGALNDRLYVLCETNLETIEEA
IGEVRPDFLVIDSIQTVYRPEVASAPGSVAQVRECTAHFMRISKVNGVATVLVGHVTKEGAIAGPRLLEHMVDCVLYFEG
ERHHTYRILRAVKNRFGSTNEIGIFDMKEDGLREVANPSELFLSERPLGVAGSTVVASMEGTRPVLVELQALVAPTHFPS
PRRMASGFDHHRMSLVIAVLEKRMGLFLQNQDAYLNAAGGVKLDEPAADLAAAVSLASSVKDAPTKPYDAIFGEVGLTGE
VRAVSRAETRVREAHKLGFKRVILPEHSMKGWQPPEGIALIGVTTVAEALKAALE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1060078 ACF3MZ_RS20390 WP_394045806.1 4500084..4501451(-) (radA) [Cohnella sp. WGS1546]
ATGGCAAAGGTCAAAATCAAGTTCGTCTGCACGCAGTGCGGGACGGAGTCTCCGAAATGGATGGGCAAATGCCCGGGCTG
CCAGGAATGGAACACGATGGTCGAGGAAACCGAGACCGAAGTGAAGGCGCTCGTCGGACGAAGCGAACAGACTCGCGCGA
AAGAAAAGCCGCGGGCGATCATACATATAGAGAGCGGGCAAGAACCGCGCATCGCGACGGGCATGGCGGAATTGAACCGG
GTGCTCGGCGGGGGGCTTGTGCCCGGCTCGCTGCTGCTCGTCGGCGGCGATCCGGGCATCGGCAAGTCGACGCTGCTGCT
GCAGACGTCCTATTCTCTCTCCGCGCAGGGCTTGAAGGTGCTGTACGTCTCCGGGGAAGAGTCGGTTCGCCAGACGAAGC
TTCGGGCCGATCGCCTCGGCGCGCTTAATGACCGGCTCTACGTGCTCTGCGAGACGAACCTGGAGACGATCGAGGAGGCG
ATCGGCGAAGTCCGGCCCGACTTTCTCGTCATCGATTCCATTCAGACGGTGTACCGGCCCGAGGTCGCTTCCGCGCCGGG
AAGCGTCGCGCAAGTCCGGGAGTGCACCGCTCATTTCATGCGCATCTCGAAGGTGAACGGAGTGGCGACGGTGCTCGTCG
GCCACGTCACGAAGGAAGGGGCGATCGCGGGCCCCCGCCTTCTCGAGCATATGGTCGATTGCGTGCTTTATTTCGAGGGA
GAGAGGCATCATACTTACAGAATCCTGCGCGCGGTGAAAAACCGGTTCGGATCGACGAACGAGATCGGCATTTTCGATAT
GAAGGAGGACGGACTGAGGGAGGTCGCGAATCCGTCCGAGCTGTTCTTGTCGGAGAGGCCGCTGGGCGTCGCGGGATCGA
CGGTCGTGGCCAGCATGGAGGGCACCCGCCCCGTGCTCGTCGAGCTGCAAGCGCTTGTGGCGCCGACGCATTTTCCTTCC
CCGCGAAGAATGGCCTCCGGCTTCGATCATCATCGGATGTCGCTCGTCATCGCCGTTCTCGAGAAGCGGATGGGCCTGTT
CCTGCAAAATCAGGACGCTTACTTGAATGCGGCGGGCGGCGTGAAGCTGGACGAACCCGCCGCGGATCTGGCGGCCGCGG
TCAGCCTCGCTTCCAGCGTCAAGGACGCGCCGACCAAGCCGTACGACGCGATATTCGGCGAAGTCGGGCTGACCGGCGAA
GTCCGTGCGGTGTCAAGAGCCGAGACGAGGGTGAGGGAGGCGCATAAGCTCGGTTTCAAGCGCGTCATATTGCCCGAACA
CAGCATGAAAGGCTGGCAGCCTCCCGAAGGGATCGCGCTTATCGGCGTGACGACCGTCGCGGAAGCGCTGAAGGCCGCAT
TGGAATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-220)

Subunit ChlI of Mg-chelatase (ChlI)

(361-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.351

100

0.655
  radA Streptococcus mitis NCTC 12261

58.15

99.78

0.58
  radA Streptococcus mitis SK321

58.15

99.78

0.58
  radA Streptococcus pneumoniae Rx1

57.93

99.78

0.578
  radA Streptococcus pneumoniae D39

57.93

99.78

0.578
  radA Streptococcus pneumoniae R6

57.93

99.78

0.578
  radA Streptococcus pneumoniae TIGR4

57.93

99.78

0.578

Multiple sequence alignment