Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   ACFW98_RS11800 Genome accession   NZ_CP170719
Coordinates   2462858..2464003 (+) Length   381 a.a.
NCBI ID   WP_015418439.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5008     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 2457858..2469003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW98_RS11780 (ACFW98_11780) - 2458756..2459184 (-) 429 WP_007407634.1 DUF1934 domain-containing protein -
  ACFW98_RS11785 (ACFW98_11785) - 2459338..2459850 (+) 513 Protein_2310 bacteriocin biosynthesis protein AlbG -
  ACFW98_RS11790 (ACFW98_11790) - 2459856..2461232 (-) 1377 WP_395096412.1 YncE family protein -
  ACFW98_RS11795 (ACFW98_11795) - 2461272..2462627 (-) 1356 WP_395096413.1 YncE family protein -
  ACFW98_RS11800 (ACFW98_11800) rapF 2462858..2464003 (+) 1146 WP_015418439.1 tetratricopeptide repeat protein Regulator
  ACFW98_RS11805 (ACFW98_11805) phrF 2463987..2464106 (+) 120 WP_015240778.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  ACFW98_RS11810 (ACFW98_11810) - 2464304..2464507 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  ACFW98_RS11815 (ACFW98_11815) - 2464521..2464724 (+) 204 WP_007407641.1 hypothetical protein -
  ACFW98_RS11820 (ACFW98_11820) - 2464759..2464944 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  ACFW98_RS11825 (ACFW98_11825) - 2465130..2465411 (+) 282 WP_045209208.1 hypothetical protein -
  ACFW98_RS11830 (ACFW98_11830) - 2465455..2465967 (+) 513 WP_031378560.1 stage II sporulation protein M -
  ACFW98_RS11835 (ACFW98_11835) - 2466556..2466774 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  ACFW98_RS11840 (ACFW98_11840) speB 2466942..2467814 (-) 873 WP_007407646.1 agmatinase -
  ACFW98_RS11845 (ACFW98_11845) speE 2467873..2468703 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45569.65 Da        Isoelectric Point: 4.5769

>NTDB_id=1058745 ACFW98_RS11800 WP_015418439.1 2462858..2464003(+) (rapF) [Bacillus amyloliquefaciens strain JP5008]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1058745 ACFW98_RS11800 WP_015418439.1 2462858..2464003(+) (rapF) [Bacillus amyloliquefaciens strain JP5008]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCATAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564

Multiple sequence alignment