Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   ACFXAA_RS13170 Genome accession   NZ_CP170717
Coordinates   2725230..2726375 (-) Length   381 a.a.
NCBI ID   WP_015418439.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5014     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 2720230..2731375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAA_RS13125 (ACFXAA_13125) speE 2720528..2721358 (+) 831 WP_003151052.1 spermidine synthase -
  ACFXAA_RS13130 (ACFXAA_13130) speB 2721417..2722289 (+) 873 WP_007407646.1 agmatinase -
  ACFXAA_RS13135 (ACFXAA_13135) - 2722457..2722675 (+) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  ACFXAA_RS13140 (ACFXAA_13140) - 2723266..2723778 (-) 513 WP_031378560.1 stage II sporulation protein M -
  ACFXAA_RS13145 (ACFXAA_13145) - 2723822..2724103 (-) 282 WP_007407643.1 hypothetical protein -
  ACFXAA_RS13150 (ACFXAA_13150) - 2724289..2724474 (-) 186 WP_031378559.1 DUF4177 domain-containing protein -
  ACFXAA_RS13155 (ACFXAA_13155) - 2724509..2724712 (-) 204 WP_007407641.1 hypothetical protein -
  ACFXAA_RS13160 (ACFXAA_13160) - 2724726..2724929 (-) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  ACFXAA_RS13165 (ACFXAA_13165) phrF 2725127..2725246 (-) 120 WP_031378557.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  ACFXAA_RS13170 (ACFXAA_13170) rapF 2725230..2726375 (-) 1146 WP_015418439.1 tetratricopeptide repeat protein Regulator
  ACFXAA_RS13175 (ACFXAA_13175) - 2726606..2727961 (+) 1356 WP_060673883.1 YncE family protein -
  ACFXAA_RS13180 (ACFXAA_13180) - 2728001..2728708 (+) 708 WP_060673885.1 YncE family protein -
  ACFXAA_RS13185 (ACFXAA_13185) - 2728715..2729227 (-) 513 Protein_2546 bacteriocin biosynthesis protein AlbG -
  ACFXAA_RS13190 (ACFXAA_13190) - 2729380..2729808 (+) 429 WP_060673887.1 DUF1934 domain-containing protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45569.65 Da        Isoelectric Point: 4.5769

>NTDB_id=1058667 ACFXAA_RS13170 WP_015418439.1 2725230..2726375(-) (rapF) [Bacillus amyloliquefaciens strain JP5014]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1058667 ACFXAA_RS13170 WP_015418439.1 2725230..2726375(-) (rapF) [Bacillus amyloliquefaciens strain JP5014]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCATAATCTAATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTATTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564

Multiple sequence alignment