Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFXAA_RS09565 Genome accession   NZ_CP170717
Coordinates   2003104..2003541 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5014     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1998104..2008541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAA_RS09550 (ACFXAA_09550) - 2000963..2001325 (-) 363 WP_007409352.1 helix-turn-helix domain-containing protein -
  ACFXAA_RS09555 (ACFXAA_09555) hxlA 2001557..2002192 (+) 636 WP_031378686.1 3-hexulose-6-phosphate synthase -
  ACFXAA_RS09560 (ACFXAA_09560) hxlB 2002189..2002746 (+) 558 WP_039252823.1 6-phospho-3-hexuloisomerase -
  ACFXAA_RS09565 (ACFXAA_09565) nucA/comI 2003104..2003541 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACFXAA_RS09570 (ACFXAA_09570) nin/comJ 2003562..2003960 (+) 399 WP_020955304.1 competence protein ComJ Regulator
  ACFXAA_RS09575 (ACFXAA_09575) - 2004001..2005437 (-) 1437 WP_060675311.1 glycoside hydrolase family 1 protein -
  ACFXAA_RS09580 (ACFXAA_09580) - 2005541..2005819 (-) 279 WP_263612330.1 YckD family protein -
  ACFXAA_RS09585 (ACFXAA_09585) - 2005937..2006371 (-) 435 WP_012116767.1 RDD family protein -
  ACFXAA_RS09590 (ACFXAA_09590) - 2006386..2007522 (-) 1137 WP_007409359.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1058655 ACFXAA_RS09565 WP_003156588.1 2003104..2003541(+) (nucA/comI) [Bacillus amyloliquefaciens strain JP5014]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1058655 ACFXAA_RS09565 WP_003156588.1 2003104..2003541(+) (nucA/comI) [Bacillus amyloliquefaciens strain JP5014]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCCCTTAAACATGTGCCTGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment