Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACFYH6_RS01325 Genome accession   NZ_CP170704
Coordinates   269751..271118 (+) Length   455 a.a.
NCBI ID   WP_416179377.1    Uniprot ID   -
Organism   Clostridium sp. HCS.1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 264751..276118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFYH6_RS01295 (ACFYH6_01295) - 265580..265804 (+) 225 WP_316343204.1 glutaredoxin family protein -
  ACFYH6_RS01300 (ACFYH6_01300) - 265817..267274 (+) 1458 WP_316343203.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  ACFYH6_RS01305 (ACFYH6_01305) - 267469..267696 (-) 228 WP_316343202.1 glutaredoxin family protein -
  ACFYH6_RS01310 (ACFYH6_01310) - 267837..267998 (-) 162 WP_316343200.1 hypothetical protein -
  ACFYH6_RS01315 (ACFYH6_01315) - 268130..268852 (+) 723 WP_416179378.1 GntR family transcriptional regulator -
  ACFYH6_RS01320 (ACFYH6_01320) nagB 268849..269574 (+) 726 WP_316343198.1 glucosamine-6-phosphate deaminase -
  ACFYH6_RS01325 (ACFYH6_01325) radA 269751..271118 (+) 1368 WP_416179377.1 DNA repair protein RadA Machinery gene
  ACFYH6_RS01330 (ACFYH6_01330) disA 271135..272199 (+) 1065 WP_316343196.1 DNA integrity scanning diadenylate cyclase DisA -
  ACFYH6_RS01335 (ACFYH6_01335) - 272268..272657 (-) 390 WP_416179376.1 DUF1573 domain-containing protein -
  ACFYH6_RS01340 (ACFYH6_01340) - 272868..273962 (+) 1095 WP_316343195.1 PIN/TRAM domain-containing protein -
  ACFYH6_RS01345 (ACFYH6_01345) ispD 273975..274643 (+) 669 WP_316343194.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50439.18 Da        Isoelectric Point: 6.2082

>NTDB_id=1058602 ACFYH6_RS01325 WP_416179377.1 269751..271118(+) (radA) [Clostridium sp. HCS.1]
MAKIKSMFVCQECGYESPKWLGKCPDCNKWNTLVEEIKETHKEAKSKVNNIRVIENMPKSIRDIKSGEKERYDTGLKELN
RVLGGGLVRGSLTLISGDPGIGKSTLLLQTADSISKSYGKVLYVSGEESEEQIKIRGDRLGVNAESLYILSETNLDLIEA
YIDEIKPVFVIIDSIQTVYKESITSAPGSVSQVKECSNAIMRIGKNQGIPLFIVAHVTKQGELAGPRVLEHMVDTVLYFE
GERTEEFRVLRTMKNRFGTTSEIGVFEMAEEGLKEIYDPSRIFLEDTNFNQEGSVVIGIMEGTRPILVEMQALVSESNTH
LASRTAVGIDNQRLRLILAVLEKKLRVPFYKYDVYVNVVGGLTLDGTTGDLGLALALLSSVKNTGLKLKRTVIIGEVGLT
GEIRPISSCERLIKEAEKMGFENAIIPERNRDKVTGSNINIIGVSNLREAINKIF

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1058602 ACFYH6_RS01325 WP_416179377.1 269751..271118(+) (radA) [Clostridium sp. HCS.1]
ATGGCAAAGATTAAATCCATGTTTGTTTGTCAAGAATGTGGATATGAGTCCCCTAAATGGTTAGGGAAATGTCCAGATTG
TAATAAATGGAATACCTTAGTTGAAGAAATTAAGGAAACACATAAAGAAGCAAAATCTAAAGTAAATAATATTAGAGTAA
TAGAAAATATGCCAAAGAGCATAAGAGATATAAAGTCAGGTGAAAAAGAAAGATATGATACTGGACTAAAAGAATTAAAT
AGAGTTTTAGGTGGTGGATTGGTAAGAGGTTCATTAACATTAATATCAGGAGATCCTGGAATAGGGAAATCTACACTGCT
TCTACAAACAGCAGACTCTATTTCAAAAAGTTATGGTAAGGTTTTATATGTTTCTGGAGAAGAATCTGAAGAGCAAATCA
AAATTAGAGGGGATAGGTTAGGAGTTAATGCAGAATCACTTTATATATTATCTGAAACTAATTTAGATTTAATAGAAGCA
TATATTGATGAGATAAAGCCTGTATTCGTAATTATAGACTCAATTCAAACTGTATATAAGGAATCTATTACTTCAGCACC
AGGAAGTGTATCTCAAGTTAAAGAGTGTTCTAATGCTATTATGAGAATAGGAAAAAATCAAGGAATACCATTATTTATAG
TTGCTCATGTAACAAAGCAAGGTGAATTAGCAGGACCAAGAGTATTAGAGCATATGGTAGATACGGTTCTATATTTTGAA
GGGGAACGTACAGAGGAATTTAGAGTTCTTAGAACTATGAAGAATCGTTTTGGTACTACTAGTGAAATTGGTGTCTTTGA
AATGGCTGAAGAAGGATTAAAGGAAATATACGATCCTTCGAGAATATTCCTAGAGGATACTAATTTTAACCAAGAAGGTT
CTGTAGTAATAGGTATTATGGAAGGTACTAGACCTATATTAGTAGAAATGCAGGCTTTGGTTAGTGAAAGTAATACTCAT
TTAGCTAGTAGAACTGCTGTAGGTATAGATAATCAAAGGTTAAGACTTATACTAGCAGTACTAGAAAAGAAATTAAGAGT
TCCTTTTTATAAATACGATGTTTATGTAAATGTAGTTGGAGGTCTTACCTTAGATGGTACTACTGGAGATTTAGGATTAG
CATTAGCACTATTATCTAGTGTTAAAAATACAGGATTAAAACTTAAAAGAACAGTTATAATTGGGGAAGTAGGATTAACG
GGAGAAATTAGACCTATATCTTCTTGTGAAAGATTAATTAAAGAAGCTGAAAAAATGGGTTTTGAAAATGCTATTATTCC
TGAAAGAAATAGGGATAAAGTTACTGGAAGTAATATAAATATAATAGGTGTAAGTAATTTAAGAGAGGCTATAAATAAGA
TATTCTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(332-430)

AAA domain (AAA_25)

(71-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

53.642

99.56

0.534
  radA Streptococcus pneumoniae Rx1

47.702

100

0.479
  radA Streptococcus pneumoniae D39

47.702

100

0.479
  radA Streptococcus pneumoniae R6

47.702

100

0.479
  radA Streptococcus pneumoniae TIGR4

47.702

100

0.479
  radA Streptococcus mitis SK321

47.702

100

0.479
  radA Streptococcus mitis NCTC 12261

46.957

100

0.475

Multiple sequence alignment