Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFXAD_RS18880 Genome accession   NZ_CP170653
Coordinates   3822818..3823255 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5011     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3817818..3828255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAD_RS18865 (ACFXAD_18865) - 3820676..3821038 (-) 363 WP_007409352.1 helix-turn-helix domain-containing protein -
  ACFXAD_RS18870 (ACFXAD_18870) hxlA 3821270..3821905 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  ACFXAD_RS18875 (ACFXAD_18875) hxlB 3821902..3822459 (+) 558 WP_053573744.1 6-phospho-3-hexuloisomerase -
  ACFXAD_RS18880 (ACFXAD_18880) nucA/comI 3822818..3823255 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACFXAD_RS18885 (ACFXAD_18885) nin/comJ 3823276..3823674 (+) 399 WP_020955304.1 competence protein ComJ Regulator
  ACFXAD_RS18890 (ACFXAD_18890) - 3823715..3825151 (-) 1437 WP_053573745.1 glycoside hydrolase family 1 protein -
  ACFXAD_RS18895 (ACFXAD_18895) - 3825255..3825533 (-) 279 WP_263612330.1 YckD family protein -
  ACFXAD_RS18900 (ACFXAD_18900) - 3825651..3826085 (-) 435 WP_012116767.1 RDD family protein -
  ACFXAD_RS18905 (ACFXAD_18905) - 3826100..3827236 (-) 1137 WP_025284207.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1058528 ACFXAD_RS18880 WP_003156588.1 3822818..3823255(+) (nucA/comI) [Bacillus amyloliquefaciens strain JP5011]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1058528 ACFXAD_RS18880 WP_003156588.1 3822818..3823255(+) (nucA/comI) [Bacillus amyloliquefaciens strain JP5011]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment