Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   ACFXAD_RS10035 Genome accession   NZ_CP170653
Coordinates   1981532..1982140 (-) Length   202 a.a.
NCBI ID   WP_012117837.1    Uniprot ID   A0AAP5V0D3
Organism   Bacillus amyloliquefaciens strain JP5011     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1976532..1987140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAD_RS10015 (ACFXAD_10015) dnaD 1976768..1977466 (+) 699 WP_003153516.1 DNA replication protein DnaD -
  ACFXAD_RS10020 (ACFXAD_10020) nth 1977485..1978144 (+) 660 WP_053573256.1 endonuclease III -
  ACFXAD_RS10025 (ACFXAD_10025) - 1978141..1978632 (+) 492 WP_053573255.1 YpoC family protein -
  ACFXAD_RS10030 (ACFXAD_10030) - 1978710..1981493 (-) 2784 WP_015417703.1 PBP1A family penicillin-binding protein -
  ACFXAD_RS10035 (ACFXAD_10035) recU 1981532..1982140 (-) 609 WP_012117837.1 Holliday junction resolvase RecU Machinery gene
  ACFXAD_RS10040 (ACFXAD_10040) - 1982182..1983144 (+) 963 WP_053573254.1 DUF2515 domain-containing protein -
  ACFXAD_RS10045 (ACFXAD_10045) sspM 1983188..1983292 (-) 105 WP_003153525.1 acid-soluble spore protein SspM -
  ACFXAD_RS10050 (ACFXAD_10050) - 1983498..1983743 (+) 246 WP_012117836.1 DUF5446 family protein -
  ACFXAD_RS10055 (ACFXAD_10055) - 1983789..1984157 (+) 369 WP_053573253.1 YppE family protein -
  ACFXAD_RS10060 (ACFXAD_10060) - 1984196..1984378 (-) 183 WP_003153532.1 YppF family protein -
  ACFXAD_RS10065 (ACFXAD_10065) - 1984565..1984999 (+) 435 WP_053573252.1 YppG family protein -
  ACFXAD_RS10070 (ACFXAD_10070) - 1985029..1985442 (-) 414 WP_053573251.1 Hsp20/alpha crystallin family protein -
  ACFXAD_RS10075 (ACFXAD_10075) - 1985581..1986087 (+) 507 WP_012117833.1 PTS glucose transporter subunit IIA -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23666.75 Da        Isoelectric Point: 9.5778

>NTDB_id=1058498 ACFXAD_RS10035 WP_012117837.1 1981532..1982140(-) (recU) [Bacillus amyloliquefaciens strain JP5011]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
NNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=1058498 ACFXAD_RS10035 WP_012117837.1 1981532..1982140(-) (recU) [Bacillus amyloliquefaciens strain JP5011]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCTCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAATATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGTGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCCACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTTGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGACAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AACAATGGCAGAAAATCGATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

90.452

98.515

0.891

Multiple sequence alignment