Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACFXAD_RS08455 Genome accession   NZ_CP170653
Coordinates   1648962..1649729 (+) Length   255 a.a.
NCBI ID   WP_007408287.1    Uniprot ID   A0AAP3YCF1
Organism   Bacillus amyloliquefaciens strain JP5011     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1643962..1654729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAD_RS08425 (ACFXAD_08425) - 1644245..1646377 (+) 2133 WP_053573187.1 HD family phosphohydrolase -
  ACFXAD_RS08430 (ACFXAD_08430) ybeY 1646378..1646851 (+) 474 WP_012118016.1 rRNA maturation RNase YbeY -
  ACFXAD_RS08435 (ACFXAD_08435) - 1646832..1647206 (+) 375 WP_003152974.1 diacylglycerol kinase family protein -
  ACFXAD_RS08440 (ACFXAD_08440) - 1647330..1647740 (+) 411 WP_003152976.1 cytidine deaminase -
  ACFXAD_RS08445 (ACFXAD_08445) era 1647721..1648626 (+) 906 WP_007408285.1 GTPase Era -
  ACFXAD_RS08450 (ACFXAD_08450) - 1648782..1648925 (+) 144 WP_007408286.1 YqzL family protein -
  ACFXAD_RS08455 (ACFXAD_08455) recO 1648962..1649729 (+) 768 WP_007408287.1 DNA repair protein RecO Machinery gene
  ACFXAD_RS08460 (ACFXAD_08460) glyQ 1650031..1650918 (+) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  ACFXAD_RS08465 (ACFXAD_08465) glyS 1650911..1652950 (+) 2040 WP_053573188.1 glycine--tRNA ligase subunit beta -
  ACFXAD_RS08470 (ACFXAD_08470) ccpN 1653051..1653689 (+) 639 WP_003152989.1 transcriptional regulator CcpN -
  ACFXAD_RS08475 (ACFXAD_08475) - 1653715..1654527 (+) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29292.91 Da        Isoelectric Point: 8.5625

>NTDB_id=1058488 ACFXAD_RS08455 WP_007408287.1 1648962..1649729(+) (recO) [Bacillus amyloliquefaciens strain JP5011]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1058488 ACFXAD_RS08455 WP_007408287.1 1648962..1649729(+) (recO) [Bacillus amyloliquefaciens strain JP5011]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAATGAAATTAAACGGGTCATTGATCTGTATTACGAAGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.02

100

0.89

Multiple sequence alignment