Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACFW99_RS17315 Genome accession   NZ_CP170651
Coordinates   3528653..3530038 (-) Length   461 a.a.
NCBI ID   WP_040238815.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3523653..3535038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS17280 (ACFW99_17280) flgL 3523828..3524745 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  ACFW99_RS17285 (ACFW99_17285) flgK 3524757..3526274 (-) 1518 WP_033575223.1 flagellar hook-associated protein FlgK -
  ACFW99_RS17290 (ACFW99_17290) - 3526290..3526772 (-) 483 WP_003151394.1 flagellar protein FlgN -
  ACFW99_RS17295 (ACFW99_17295) flgM 3526787..3527053 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACFW99_RS17300 (ACFW99_17300) - 3527123..3527542 (-) 420 WP_025285352.1 TIGR03826 family flagellar region protein -
  ACFW99_RS17305 (ACFW99_17305) comFC 3527616..3528305 (-) 690 WP_197093073.1 phosphoribosyltransferase family protein Machinery gene
  ACFW99_RS17310 (ACFW99_17310) - 3528311..3528595 (-) 285 WP_003151389.1 late competence development ComFB family protein -
  ACFW99_RS17315 (ACFW99_17315) comFA 3528653..3530038 (-) 1386 WP_040238815.1 DEAD/DEAH box helicase Machinery gene
  ACFW99_RS17320 (ACFW99_17320) - 3530146..3530994 (-) 849 WP_015418336.1 DegV family protein -
  ACFW99_RS17325 (ACFW99_17325) degU 3531092..3531781 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  ACFW99_RS17330 (ACFW99_17330) degS 3531858..3533021 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  ACFW99_RS17335 (ACFW99_17335) - 3533244..3533891 (+) 648 WP_007407485.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52515.94 Da        Isoelectric Point: 10.0818

>NTDB_id=1058441 ACFW99_RS17315 WP_040238815.1 3528653..3530038(-) (comFA) [Bacillus amyloliquefaciens strain JP5025]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWASSAKRQIT
YCRACVMMGRADELTSLYSWNQASENSWEPVKLSWEGTLTDGQKKAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
CQKLQSQLPVFLFVPSVDVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGMTNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1058441 ACFW99_RS17315 WP_040238815.1 3528653..3530038(-) (comFA) [Bacillus amyloliquefaciens strain JP5025]
ATGTCCGAGGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAAGCGTTTTATAGAAAAAGAGTATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGTCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCTCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGAAGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTATGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTT
TGCCAAAAACTTCAATCACAGCTGCCTGTTTTCTTATTCGTTCCGTCTGTAGACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACCGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATGACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(325-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

63.261

99.783

0.631

Multiple sequence alignment